The study of plant regeneration from root explants of Jatropha curcas and origin of adventitious shoots

Abstract:

In order to overcome the shortcomings of the existing propagation technology of Jatropha curcas, adventitious buds were induced from the adventitious root explants of cotyledon and radicle explants respectively. The optimal way to get adventitious root from cotyledons of Jatropha curcas L. was that it would be precultured on the MS medium containing 5 mg/L IBA for 4 days, then transferred to MS0 medium for rooting. The rooting inducing rate and adventitious root number reached 93.33% and 6.54/explant, respectively. The optimal medium for adventitious buds inducing was MS+3.0 mg/L 6-BA+3 mg/L IAA. The induction rate could reach 83.33%. The optimal adventitious bud induction medium from the radicle explants of Jatropha curcas L. was MS+0.2 mg/L 6-BA+1.0 mg/L IBA. The differentiation rate was 67.80%, and the average number of sprouts was 3.25 per explant. Paraffin sections were observed at each stage of bud regeneration and it seems that adventitious buds originate from parenchyma of roots. Compared with the prior methodology, the method presented here has the characteristics of a shorter cycle and higher regeneration efficiency.