Study on the mechanism of Cordyceps cicadae extracts promote longevity in Saccharomyces cerevisiae

Abstract:

This study aims to explore the role of Cordyceps cicadae extracts (CCE) in resistance against H2O2 -induced oxidative stress and chronological lifespan extension in Saccharomyces cerevisiae. The experiments are performed with various concentrations of CCE to test the chronological lifespan extension. Then, the resistance against H2O2 induced oxidative stress was measured in terms of intracellular ROS accumulation. The mRNA levels of SOD2, GPX2 and CTT1 were measured by real -time PCR. The results indicate that CCE can prolong yeast chronological lifespan in a dosedependent manner. Furthermore, the CCE pre-treated cells accumulate significantly lower ROS in the case of H2O2-induced oxidative stress. Taken together these results suggest that CCE extends chronological lifespan and develops resistance against H2O2 induced oxidative stress through upregulation of CTT1 and SOD2.