Bile bacterial diversity in patients with cholelithiasis and the sphincter of Oddi laxity based on high-throughput sequencing

Abstract:

To study the composition and diversity of bile bacteria in patients with the sphincter of Oddi laxity (SOL), to provide basis for clinical treatment of cholelithiasis recurrence in patients with SOL, this study analyzed the differences of clinical indexes before operation, including liver and kidney function and blood routine index between control (non-SOL patients with cholelithiasis) and SOL group. The high-throughput 16S rRNA gene sequencing of biliary bacteria was performed based on the Illumina MiSeq platform. The results showed that the level of total bilirubin, direct bilirubin and indirect bilirubin in cholelithiasis patients with the SOL were significantly higher than those in the nonSOL group, while adenosine deaminase (ADA) was lower than that in control (P< 0.05). There was no significant difference in bacterial alpha diversity between SOL and non-SOL patients except for evenness (P> 0.05), and Wilcoxon rank-sum test showed that the top four abundant bacterial phyla in SOL patients bile were Proteobacteria, Firmicutes, Actinobacteria and Bacteroidete in turn, among which the abundance of Proteobacteria, Enterococcus and Lachnoclostridium was significantly higher in SOL patients than in control (P< 0.05), so did Klebsiella, Massilia and Pseudomonas (P>0.05). Therefore, there are significant differences in some clinical indexes such as bilirubin, ADA level and bile bacterial composition between patients with SOL and those without SOL, which provide relevant basis for the clinical treatment of cholelithiasis recurrence in patients with SOL.