Effects of Napabucasin on the proliferation and migration of colorectal cancer cells

Abstract:

This study aims to explore the effect of the novel small molecule inhibitor Napabucasin on the proliferation and migration of colorectal cancer cells. Firstly, the interaction mechanism between Napabucasin and STAT3 was analyzed by molecular docking. Then, it was proved that Napabucasin can significantly inhibit the colony formation and migration ability of colorectal cancer cells using clone formation experiments and wound healing assay. Napabucasin and Wnt agonist 1 were then used to cotreat the HCT116 cells, combined with Western blot experiments, it was found that the Wnt signaling pathway mediates the inhibition of the migration and proliferation of colorectal cancer cells by Napabucasin. The research results show that Napabucasin can inhibit the proliferation and migration ability of colorectal cancer cells in vitro, and the Wnt signaling pathway is involved in mediating this inhibition process.