Research on microbial diversity in the Deep Strata of JinPing based on high-throughput sequencing

Abstract:

This study aims to explore the composition and function of the microbial community of the deep strata in Jinping. The high-throughput sequencing was used to investigate the microbial diversity and the microbial populations in the deep strata of Jinping. A total of 732,477 clean tags were obtained and clustered to 1,318 effective OTUs based on 97% sequence similarity.Though there were certain differences among the microbiota of each samples. The dominant bacteria in phylum remained the same, which are Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. PICRUSt analysis was performed to show that abundance of the functional genes higly involved in Carbohydrate metabolism, Amino acid metabolism, Energy metabolism and Metabolism of cofactors and vitamins. The research reveals that the phylogenetic diversity of the microbial communities in the deep strata of Jinping is complex, but the main microbial communities are relatively stable.