Screening of active components of Rhododendron dauricum L. against Trichophyton rubrum

Abstract:

To explore the main active components that inhibit T.rubrum in the leaves of Rhododendron dauricum L., 16 compounds were isolated and identified from the ethyl acetate phase of Rhododendron dauricum L.’s leaves by a variety of isolation methods based on the bioactivity tracking method.They are identified as Daurichromenic acid(1), farrerol(2), vanillic acid(3), taxifolin(4), 4-Hydroxybenzoic acid(5), umbelliferone(6), 2-Methyl-5,7-dihydroxychromone(7), Maslinic acid(8), corosolic acid(9), avicularin(10), syringic acid(11), kaempferol(12), quercetin(13), ursolic acid(14), oleanolic acid(15),cucurbitacin D(16).Among them,compounds 7~9 were isolated from this plant for the first time.The results of bacteriostatic activity determination showed that compounds 1~3, 5~7 and 11 had certain inhibitory activities against T.rubrum.Among them, compounds 2, 6 and 7 had the highest inhibitory activity and were the main active substances, with MIC of 0.15625~0.3125mg·mL-1 and MFC of 1.25~5 mg·mL-1.In this study, it was first found that leaves of R.dauricum L.had better inhibitory activity against T.rubrum and the active monomer was screened out.