Study on YAP gene silencing activity of phosphorothioate siRNAs

Abstract:

In order to explore the gene silencing activity of diastereomeric pure PS-siRNA (pPS-siRNA) synthesized enzymatically by RNA polymerase, its silencing activity on Yes-associated protein (YAP), a potential cancer treatment target, and cytotoxicity to HeLa cells were studied by qPCR and Western blot in this study. The data showed that compared with unmodified siRNAs (PO-siRNAs), pPS-siRNAs can better reduce YAP gene expression without significant cytotoxicity (approximately 30% more efficient). In addition, MTT cell proliferation assay and flow cytometry showed that the proliferation of HeLa cells was inhibited after YAP down-regulated by the pPS-siRNAs. This research proved the superiority of pPS-siRNAs in gene therapy and the potential of YAP as a tumor therapeutic target.