Assembly and characteristic analysis of Chara globularis chloroplast whole genome

Abstract:

In order to reveal the characteristics of the entire chloroplast genome of Chara globularis and explore its phylogenetic relationship in Characeae, highthroughput sequencing technology was used to assemble and sequence the entire chloroplast genome. The study showed that C. gloublaris is a circular molecule of 180 652 bp in length with a typical quadripartite structure and a 26.6% GC-content. 137 chloroplast genes were successfully annotated, including 94 protein-coding genes, 37 tRNA genes and 6 rRNA genes. There are 2 more protein-coding genes and 3 more tRNA genes than Nitella hyalina. Compared to land plants, C. globularis has four special genes: rpl12, trnL(gag), rpl19, and ycf20. A total of 87 SSR loci were detected in the genome, and most of them were composed of A and T. In addition, C. gloublaris contains a total of 24 989 codons and the codon usage prefers A and T. Leucine (Leu) is the codon that encodes the most amino acids. The phylogenetic tree constructed by the Neighbor Joining(NJ) on the entire chloroplast genomes of 5 species including C. gloublaris. showed that the phylogenetic relationship of C.gloublaris was closer to that of Chara vulgairs. This study analyzed the entire chloroplast genome of Chara globularis, and established its phylogenetic status by using the existing data.