Genome-wide identification and expression analysis of protein phosphatase 2C family in Tartary buckwheat

Abstract:

In order to study the members and classification of protein phosphatase 2C (PP2C) family in Tartary buckwheat and to explore its function in the growth and development of Tartary buckwheat, this study used bioinformatics methods to identify and classify the PP2C genes of Tartary buckwheat (FtPP2C), and its genes structure, conservative motifs, and molecular evolution were analyzed. The results revealed that 81 FtPP2C genes divided into 11 subgroups (A-K), and the sequence characteristics in the same subgroups were similar, but there were certain differences in sequence characteristics among different subgroups. The results also revealed that FtPP2C genes had 14 gene duplication events. In addition, the expression patterns of FtPP2C clade A genes were analyzed by qRT-PCR, and the results revealed that all FtPP2C clade A genes were expressed in five organs (roots, stems, leaves, flowers, and fruits), among them, 8 genes except FtPP2C44 were highly expressed in flowers and fruits; in seedlings, all 8 genes except FtPP2C08 were up-regulated after ABA treatment. The above results revealed that the composition, sequence characteristics, and amplification of the FtPP2C genes. These results also revealed the tissue expression pattern and the expression induced by ABA of FtPP2C clade A genes.