Study on characteristics of matter transformation of Tremella fuciformis in factory cultivation with fresh Juncao

Abstract:

In order to explore the mechanism of factory cultivation of Tremella fuciformis with fresh juncao as the main substrate, GC-MS was used to analyze the substance transformation characteristics during 3 different stages，which were G1 (hypha growth phase, 12 d after inoculation), G2 (primordium differentiation stage, 24 d after inoculation) and G3 (fruit body growth phase, 36 d after inoculated). After main component analysis, partial least squares discriminant analysis, and orthogonal partial least squares discriminant analysis, the results showed that there were 426, 423, and 466 different metabolites in G2 than G1, G3 than G2, and G3 compared to G1, respectively. The carbohydrate content in G2 and G1 was higher than that in G3. The content of fat and terpenoids increased in G3 than that in G1. Differential metabolites were screened and metabolic pathways were matched. The differential pathways were fatty acid synthesis, glyoxylic acid and dicarboxylic acid metabolism, and the citric acid cycle. The synthesis pathways of unsaturated fatty acids were significantly different between G2 and G1. The metabolic pathways of alanine, aspartic acid and glutamate were significantly different between G3 and G2.The metabolic pathways of glycerol metabolism were significantly different between G3 and G1. All the results indicated that there were significant differences in substance transformation and metabolites in different growth stages of Tremella fuciformis.