Plant lipid synthesis-related genes function verification by TRV overexpression and silencing systems

Abstract:

In order to explore the possibility of tobacco rattle virus overexpression and silencing system for oil-related gene research, this study cloned Arabidopsis thaliana WRI1 and FAD2 genes, and constructed TRV overexpression and silencing vectors, respectively. The transient infection technique was used to infect Nicotiana benthamiana, and the material was collected for RT-PCR and fatty acid content detection. The results showed that the WRI1 gene and related fatty acid synthesis genes ACP1, KAS1, and BCCP2 in both infected and non-infected leaves were significantly up-regulated in Nicotiana benthamiana overexpressing the WRI1 gene, and the fatty acid content test results showed that the levels of fatty acids increased 16% and 28%, respectively. In Nicotiana benthamiana plants that silenced the FDA2 gene, the content of polyunsaturated fatty acids was found to be reduced by approximately 25% and 24%, respectively. Therefore, it is possible to use TRV system to study lipid related genes.