Transcriptome analysis of downstream gene expression regulated by deacetylase in Arabidopsis thaliana under salt stress

Abstract:

Stress refers to the ability of plants to establish protective and adaptive mechanisms under continuous exposure to environmental stimuli. As an epigenetic phenomenon, histone deacetylation plays an important role in plant adaptation. In order to explore the role of histone deacetylase in plant resistance and adaptation to high salt stress, wild-type and h2tq (HD2 four mutants) of Arabidopsis thaliana were used in this study. RNA-seq and bioinformatics methods were used to analyze the transcriptome of 10 day old seedlings before and after high salt (150 mmol/L NaCl) treatment, and the reliability of transcriptome data was verified by real-time quantitative PCR. The results showed that there were 25 differentially expressed genes in WT and hd2q, including 2 up-regulated genes and 23 down-regulated genes. After high salt treatment, there were 1407 differentially expressed genes in WT and hd2q, including 772 up-regulated genes and 635 down- regulated genes. Go and KEGG analysis showed that the control genes were mainly enriched in the extracellular region and in response to abscisic acid, while the salt treated genes were mainly enriched in the extracellular region and cell wall. Response to salt stress is a complex process involving different cross pathways. These results can provide some reference for the study of epigenetic response to salt stress.