Tissue culture and alkaloids determination of Fritillaria unibracteata Hsiao et K.C.Hsia var. wabuensis

Abstract:

In order to alleviate the contradiction between market supply and demand of Fritillaria cirrhosa, the regenerated bulbs of Fritillaria unibracteata Hsiao et K. C. Hsia var. wabuensis were produced by tissue culture technology in this study, the suitable tissue culture conditions were also explored, and the total alkaloids were determined by acid dye colorimetry. The results showed that the bulbs were sterilized with 75 % ethanol for about 10 s and 0.1 % HgCl2 for 15 min with the lowest explants contamination rate. Different types and concentrations hormones can affect the initiation and growth of explants, the concenteation range of NAA and 6-BA were controlled at 0.5~2.0 mol/L, respectively, and both can promote the growth of regenerated bulbs. The contents of total alkaloids in regenerated bulbs of Fritillariaunibracteata Hsiao et K. C. Hsia var. wabuensis were determined. Under different hormone culture conditions, there were differences in the total alkaloids of regenerated bulbs of Fritillaria unibracteata Hsiao et K. C. Hsia var. wabuensis, and the total alkaloids of more than half of the regenerated bulbs in tissue culture met the requirements of Chinese Pharmacopoeia. Therefore, tissue culture is an effective way to obtain the active ingredients of Fritillaria unibracteata Hsiao et K. C. Hsia var. wabuensis.Fritillaria unibracteata Hsiao et K. C.

Key words:Hsia var. wabuensis; Tissue culture; Plant hormones; Alkaloids