Research on the interaction between CPK11 and ABF4

Abstract:

To investigate the way that the calcium-dependent protein kinase CPK11 involved in regulating ABA signal transduction, Yeast two hybrid (Y2H) assay and Bimolecular fluorescence complementation (BiFC) assay were used to analyze the relationship between CPK11 and ABA-responsisve element binding factors ABF4. Y2H assay showed that CPK11 and ABF4 had an interaction in vitro, and BIFC assay showed that CPK11 and ABF4 had an interaction in vivo. The above experiments together proved that there was a direct interaction between CPK11 and ABF4. As a homologous protein of CPK11, CPK4 also interacted with transcription factor ABF4 in plants. In summary, these results indicated that CPK11 and its homologous protein CPK4 may participate in calcium-mediated ABA signaling pathway by interacting with transcription factor ABF4.