Abstract:In order to explore the molecular mechanism of Agaricus bisporus cooling and fruiting, this experiment took Agaricus bisporus W192 as the object. High-throughput RNA sequencing technology was used to analyze the gene expression of mycelium treated with the ambient temperature lowered from 21.5 ℃ to 17.5 ℃ at a constant rate for 4 d, 6 d and 8 d. There were 1481 differentially expressed genes in 6 d, which were 6.9% and 34% higher than 8 d and 4 d, respectively. Functional clustering analysis of GO Ontology (GO Ontology) showed that differential genes were more distributed in cellular components and biological processes, of which 6 d was dominant in metabolic and cellular processes. In addition, the differential genes under stress response and fruit body differentiation items were obviously and basically up-regulated. However, no such differentially expressed genes appeared on 4 d and 8 d, and the differentially expressed genes on 6 d were more obviously involved in the metabolism of various amino acids and sugars in cells. Functional enrichment analysis of KEGG pathway showed that the differentially expressed genes were mainly enriched in amino acid and antibiotic biosynthesis pathways, of which 6 d was enriched in glycolysis and ribosomal biosynthesis pathways, while 4 d and 8 d did not. On 4 d and 6 d, the cooling stimulation was easy to produce high yield, but on 4 d, the fruiting was early, the density was high, and the stratification was not obvious which would affect the mushroom type. On 6 d, the yield and quality were more stable. This study revealed the expression patterns of mycelium under different cooling stimuli, which provided a scientific theory for environmental regulation of industrial Agaricus bisporus during bud stimulating stage.
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