Genome-wide analysis of the R2R3-MYB transcription factor family in Salix suchowensis

Abstract:

In order to study the taxonomy and evolution of the R2R3-MYB transcription factor family members and their possible functions under gravistimulation, this study used genome-wide identification strategy and other bioinformatics methods to analyze the related family members of Salix suchowensis in detail. The results showed that a total of 158 R2R3-MYB proteins were identified in Salix suchowensis. These members were further divided into 23 subgroups. The analysis of gene structure and motif composition showed that genes in the same subgroup usually had similar exon/intron structure and similar motif composition, which further supported the results of phylogenetic analysis. Syntenic analysis was performed to explore the evolution of the SsMYB family. 85 pairs of SsMYB genes were predicted to arise from tandem or segment duplication events, which played an important role in the expansion of the SsMYB family. In addition, RNA-seq data was used to analyze the expression pattern of SsMYB genes under gravistimulation, and 20 potential SsMYB genes were screened out which involved in negative gravitropism of the stem.