Study on chemical constituents of Chlorophyll nonsaponifiable matter in Faeces Bombycis

Abstract:

Chlorophyll non-saponifiable matter is a by-product with a large yield in the process of producing chlorophyll from Faeces Bombycis. To further enhance the comprehensive utilization value of Faeces Bombycis, this research focuses on the chemical constituents in chlorophyll non-saponifiable matter of Faeces Bombycis. Silica gelcolumn chromatography, C18 medium pressure column chromatography, high pressure preparative column chromatography was used for separation and purification. The structures of the compounds were identified by spectroscopic data and physicochemical properties. A total of 11 compounds were isolated and identified, which were phytol(1),5-glutinen-3-ol(2), lupeol(3), stigmasterol(4), campesterol(5), β-sitosterol(6), α-tocopherol(7), γ-tocoferol(8), (2E,6E,10E,14E,18E,22E)-3, 7, 11, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-tetracosahexaen-1-ol(9), 13, 14, 17, 20R)-lanosta-7, 24-diene-3-ol(10), palmitic acid(11). According to the results, the main components of the non-saponifiable matter are rich in sterols, and compounds 2, 9 and 10 were isolated from Faeces Bombycis for the first time.