Metabolome studies of root exudates from different clubroot resistance Brassica napus

Abstract:

To investigate the metabolites in host root exudates that are important for the germination of Plasmodiophora brassicae resting spores, in this study, we first tested the induction ability of resting spores’ germination by root exudates from different clubroot resistant levels materials of Brassica napus, it was found that the germination rate induced by the root exudates of the susceptible materials was significantly higher than that of the resistant materials. Then, we analyzed the root exudates of two resistant and one susceptible material by non-targeted liquid chromatography-mass spectrometry metabolite profiling, non-redundant mass ions (1079 in ESI- mode and 1257 in ESI+ mode) were identified, of which 662 and 722 differential metabolites were screened in ESI- and ESI+ respectively. Some of the compounds with large differences were selected to be further verified. The results showed that Thymine, 2′-Deoxyuridine, L-isoleucine, Trigonelline, Pyridoxal 5′-phosphate, Coumarin, D-lyxose, and Amentoflavone had a significant promoting effect on the germination of resting spores and subsequently caused the development of clubroot.

Key words:Brassica napus; Clubroot resistance; Root exudates; Metabolome