Genome-wide analysis of TPS gene family of Zanthoxylum armatum

Abstract:

In order to explore the potential function of the TPS gene family in perfume plant, a comprehensive investigation of the TPS gene family was conducted in the well-known perfume plant Zanthoxylum armatum, and the basic physical and chemical properties, gene duplication type, subcellular localization, evolutionary relationship, chromosome localization and collinear analysis, gene structure and gene expression pattern were analyzed. A total of 53 ZaTPS genes were identified in the whole genome of Z. armatum. The amino acid length of their proteins ranges from 173 to 859 aa, and the molecular weight ranges from 20.21 to 98.44 kDa. The isoelectric point ranges from 4.87 to 9.10, and the subcellular localizations are mainly in the cytoplasm and chloroplast. Phylogenetic analysis showed that ZaTPSs could be divided into six subfamilies, and the members of TPS-a and TPS-b subfamilies were the majority ones. There are 48 ZaTPSs containing motif1 that are conserved in the TPS proteins. Chromosomal localization and collinear analysis showed that 53 ZaTPSs were unevenly distributed on 11 chromosomes, and there were four pairs of tandem repeats, and the collinear analysis revealed that TPS genes in Z. armatum and Citrus sinensis from the same family have closer evolutionary relationships. The gene differential expression analysis based on transcriptome datasets showed that ZaTPSs had tissuespecific expression patterns, with the highest expression level was found in young flowers, followed by husks. These results indicate that ZaTPSs played an important role in the synthesis of husks volatile oil and defense.