Detection and diversity analysis of viruses infecting Panax notoginseng in Yunnan province, Sichuan province and Chongqing municipality

Abstract:

To investigate the occurrence of viral diseases, the outbreak regularity and virus diversity in Panax notoginseng planting areas in Yunnan province, Sichuan province, and Chongqing municipality, the suspected disease samples collected in the fields were detected by reverse transcription polymerase chain reaction (RT-PCR) / PCR. The results showed that viral diseases occurred in all Panax notoginseng planting areas, and the detection rate of the virus showed an upward trend year by year. Among them, the positive detection rate of CMV and PnVA was 51.04% and 63.54%, respectively, and the combined infection rate of the two viruses was 32.29%. Then, the sequence alignment and phylogenetic analysis of coat protein gene (CP) of cucumber mosaic virus (CMV) and panax notoginseng virus A (PnVA) were carried out. The results showed that there were differences in the CP gene of virus isolates from different Panax notoginseng planting areas.