Determination of Six Flavonoids in Agrimonia Pilosa Ledeb by HPLC

Abstract:

Abstract： Objective: The HPLC method is established for separation and determination of epicatechin, gallic acid, catechin, rutin, myricetin, quercetin and kaempferol in Toona sinensis by ultrasonic extraction. Methods: The separation of seven active ingredients is performed on Phenomenex C18 column (150 mm×4.6 mm, 5 μm) with step gradient. Mobile phase is methanol -0.1% acetic acid at the flow rate of 0.8 mL﹒min-1 ; the UV detection wavelength is 290 nm and the coclumn temperature is set at 35℃. Results: The good separation of epicatechin, gallic acid, catechin, rutin, myricetin, quercetin and kaempferol is achieved within 15 min. Calibration curves of the seven active ingrediens show linear relationship (r>0.9991, n=7). The average recoveries are within 99.23%～104.5% (RSD%<1.26%, n=3). Conclusion: This ultrasonic extraction method is simple, fast, economical and reliable.