• Volume 53,Issue 1,2016 Table of Contents
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    • >Mathematics
    • Finite element method for the generalized Rosenau equatoin

      2016, 53(1):1-6.

      Abstract (2180) HTML (0) PDF 492.03 K (1254) Comment (0) Favorites

      Abstract:In this paper,we present finite element method for the generalized Rosenau equation.We prove the uniqueness and existence solutions of the fully discretization and derive related optimal error estimates.Finally,we give example to show our method is stable and efficient.

    • The dual and trace bases of the type (4,7) Gauss normal basis over finite fields

      2016, 53(1):7-12.

      Abstract (2320) HTML (0) PDF 387.58 K (1403) Comment (0) Favorites

      Abstract:Let q be a power of the prime p and let Fqbe the finite field with q elements.Recently, by characterizing some properties of cyclotomic numbers, they obtain the explicit formula for the complexity of a class of Gauss period normal bases over finite fields.Furthermore, they show that the type 7 Gauss period normal basis of Fqn over Fq is just the desired basis except for n = 4. In this paper, we compete this result and obtain the explicit formula for the complexity of 7 type Gauss period normal bases of Fq4 over Fq.

    • Periodic solutions for a kind of prescribed mean curvature Rayleigh equation with a deviating argument

      2016, 53(1):19-24.

      Abstract (2068) HTML (0) PDF 211.11 K (1154) Comment (0) Favorites

      Abstract:In this paper, the prescribed mean curvature Rayleigh equation with a deviating argument $$(\frac{u'(t)}{\sqrt{1+(u'(t))^2}})'+f(t,u'(t))+g(u(t-\tau(t)))=p(t)$$ is studied,and we prove that the given equation has at least one $T-$periodic solutions by using Mawhin's continuation theorem.

    • On Rational Distance Point Problem for Quadrilaterals

      2016, 53(1):31-34.

      Abstract (2172) HTML (0) PDF 381.71 K (1309) Comment (0) Favorites

      Abstract:In the present paper, by pointing out some errors in reference [17], we know that the well-known integer distance point problem of Steinhaus is still open. Using the result of Galois extension, we discuss the Steinhaus problem for some quadrilaterals. In fact, we show that for the case of quadrilaterals which the sum of a pair of corner is \frac{2k\pi}{n}, where n\geq 7, k

    • Positive solutions of third-order ∞-point boundary value problems

      2016, 53(1):35-41.

      Abstract (2131) HTML (0) PDF 211.67 K (1588) Comment (0) Favorites

      Abstract:In this paper, we study the existence of positive solutions to the third-order ∞-point boundary value problem u'''+ λa(t)f(u) = 0, t ∈ (0,1),u(0) = βu'(0), u(1) =∑αiu(ξi), u'(1) = 0,where λ > 0 is a parameter, ξi∈ (0,1), αi∈ [0,+∞], and satisfy ∑αi>1,0<∑αiξi(2−ξi) < 1. a(t) ∈ C([0,1],[0,∞)), f ∈ C([0,∞),[0,∞)).By using Krasnoselskii’s fixed point theorem in cones, we can obtain the existence of the positive solution and the eigenvalue intervals on which there exists a positive solution if f is either superlinear or sublinear.

    • ON SOME BASIC ARITHMETIC PROPERTIES OF A CLASS OF Q-CURVES

      2016, 53(1):42-46.

      Abstract (1942) HTML (0) PDF 1.05 M (902) Comment (0) Favorites

      Abstract:In this paper, we construct elliptic curves with complex multiplications by the integer ring of the quadratic imaginary field of discriminant -D for any positive integer D congruent to 3 modulo 4 and establish their basic properties. Our results generalize those of Gross.

    • >Computer Science
    • Image enhancement new model based on rational-order Partial Differential

      2016, 53(1):47-53.

      Abstract (2235) HTML (0) PDF 1.35 M (1277) Comment (0) Favorites

      Abstract:In order to sharpen the image edge features while enhancing texture detail, the image enhancement model based on space rational-order derivative was constructed by the effective combination of integer-order theory and fractional-order calculus theory to derive the differential expression of rational-order partial differential, and the numerical of enhanced model was achieved using rational-order partial differential mask operator. The experimental results showed that compared with the fractional-order differential method , the image enhancement model can be obtained the effect of continuous variation, that not only enhanced image texture well, but also improved the edge enhancement effect. Objectively, using image edge evaluation parameters of texture features such as information entropy and average gradient for quantitative analysis and experimental verification shown that the new model combined the advantages of integer and fractional order differential to make up for their lack of own and achieved a good image enhancement results.

    • Robust consensus for fractional-order uncertain multi-agent systems with nonlinear dynamics

      2016, 53(1):54-60.

      Abstract (199) HTML (0) PDF 1.59 M (214) Comment (0) Favorites

      Abstract:This paper investigates the robust consensus problem for uncertain fractional-order multi-agent systems with nonlinear dynamics. According to the frequency distributed equivalent model of fractional-order systems and the Lyapunov stability theory, it is proved that under the consensus protocol, the multiple agents in the network can eventually converge to the given objective state when the feedback gain matrix satisfies a certain LMI condition. The simulation results demonstrate the effectiveness and validity of the proposed method by using the fractional calculus predictor-corrector algorithm.

    • Automatically generating visually pleasing Information Walls

      2016, 53(1):61-66.

      Abstract (2130) HTML (0) PDF 1.35 M (1354) Comment (0) Favorites

      Abstract:Information Wall (IW) is a GUI composed of rectangular cells each contains various kinds of information. The design and implementation of IW are mainly in a manual way nowadays, which typically leads to less variability in appearance style and low efficiency. This paper develops a tool to automate the generation of text-IWs based on a genetic programming algorithm. A model, which evaluates the visual quality of text-IWs, is also proposed and incorporated in the algorithm to make generated IWs more visually pleasing. Test results show that the tool is able to efficiently produce good looking IWs with variable appearance styles. With the help of the tool, designer productivity could be improved by utilizing machine-generated prototypes.

    • Research of Internal Image Move-Copy Tampering Forensics Algorithm

      2016, 53(1):67-72.

      Abstract (2164) HTML (0) PDF 1.59 M (1484) Comment (0) Favorites

      Abstract:According to the low robustness characteristics of image tampering with evidence of general move-copy algorithm, this paper proposes a new algorithm. Firstly, it uses the color invariant feature to detect feature points, which improves recall forensic results. Secondly, it uses the RGB color channel to expansion Surf descriptor, which increases the robustness of feature vectors. Then, it defines noise sensitivity coefficients to identify and deal with the image noise points and use sequential similarity detection algorithm to improve feature points registration. Finally, it uses Kendall coefficient and the four hypothesis to constrain the forensic results and get the final result. Experimental results show that the new algorithm reflects higher accuracy and strong robustness and it has strong ability of forensics.

    • Topology analysis of Chinese airspace domestic flights

      2016, 53(1):73-78.

      Abstract (2096) HTML (0) PDF 1.29 M (1449) Comment (0) Favorites

      Abstract:The research of ad-hoc network based on Chinese airspace flight topology will help to meet the needs of future flights on aspects of air traffic control and information sharing,but the existing studies has not analysis the flights topology according to the specific condition of Chinese airspace. This paper collects real Chinese airspace domestic flights data and divides the Chinese airspace into east part,west part, and flight information region with NS2 simulation,and compares Chinese airspace flight topology within one day and 24 hours. The results showed that the distributions of Chinese domestic flights have big differences with each other in the Chinese airspace,however,the number of the flights shows a relative high linear correlation with the population and the third output.

    • Research on Encryption of Digital Image Based on Improper Fractional-order Laser Chaos

      2016, 53(1):79-86.

      Abstract (2075) HTML (0) PDF 3.08 M (906) Comment (0) Favorites

      Abstract:To improve the safety of information encryption, based on an improper fractional-order laser chaos, a digital image encryption algorithm is proposed. The key space is formed by initial state variables, parameters and orders of the system. Every pixel value is included in secret key, so as to improve anti-attack capability of the algorithm. Lena gray image and Couple color image are encrypted in numerical simulation. Theoretical analysis and simulation results show that this algorithm with a sufficiently large key space not only can perfectly resist the ciphertext attack and differential attack, but also is excellent against chosen plaintext attack and statistical attack, which can be used in secure communication of multimedia data.

    • >Electronics and Information Science
    • Quantum authentication and key agreement scheme for SIP protocol

      2016, 53(1):87-92.

      Abstract (2126) HTML (0) PDF 1.15 M (1586) Comment (0) Favorites

      Abstract:To overcome the shortcomings of current SIP safety scheme, such as the incapability to detect the wiretap and the vulnerability of the classical key system in case of quantum computation attack, this paper proposed a novel user authentication and key agreement scheme that combined quantum safe communication methods and SIP protocol. The SIP server prepared the three particle W states and distributed two of them to the SIP users. The random inserted particles were measured first to detect the wiretap; then the rest of the W states were operated and measured to authenticate the user. After that, every particle of the W states was measured randomly and the two SIP users got the initial conversation key according to the results of the effective measurements. The performance analysis shows our scheme is capable of resisting the impersonation attack and wiretap attack, thus can increase the safety of SIP protocol effectually.

    • Time Variation Characteristics and Assessment of Electromagnetic Environment in a Campus

      2016, 53(1):93-97.

      Abstract (2269) HTML (0) PDF 2.02 M (1866) Comment (0) Favorites

      Abstract:With the increasing complexity of the electromagnetic environment (EME), it is necessary to perform actual measurement and evaluation of the EME. Because of the temporal dynamic and random variation of the EME, time variation characteristics of the EME in a campus are considered in this paper. By choosing sampling points in different regions of the campus, respectively measuring electric field value of the sampling points during different time periods, and using statistical analysis of the measurement data, we get the figures of probability density distribution and time variation curve of the electric field. And then, the time variation characteristics of the actual EME are analyzed and discussed, the distributions of the EME in different regions are compared, as well as a brief evaluation of the electromagnetic radiation level in the campus is given.

    • A novel error resilience scheme for key frames in Wyner-Ziv video coding

      2016, 53(1):98-104.

      Abstract (2181) HTML (0) PDF 1.50 M (1251) Comment (0) Favorites

      Abstract:In Wyner-Ziv video coding system, correct transimission and accurate decoding of key frames would affect the whole system performance directly. However, in wireless communication system, data loss of key frames often happened due to network failure, network congestion or other reasons. Data lossy seriously affect the decoding quality. This paper presents a novel solution for the data loss of key frames under the conditions of feedback channel. In the solution, by sending some extra Wyner-Ziv code streams along with the origin key frames, errors of the damaged image at the decoder can be high-efficiently corrected and restored. Simulation results show that the proposed solution could reconstruct the damaged image effectively, and the quality could be improved up to 20dB compared with typical error concealment method when the data loss ratio is 1%~20%. Furthermore, R-D performance of key frames could be improved even if the data loss ratio is very high.

    • Stochastic resonance phenomenon in an active low-pass filter circuit with signal-modulated trichotomous noise

      2016, 53(1):105-109.

      Abstract (2151) HTML (0) PDF 2.66 M (1662) Comment (0) Favorites

      Abstract:The stochastic resonance phenomenon in a low-pass filter circuit driven by a multiplicative and signal-modulated trichotomous noise is investigated. Under linear system theory, applying the statistical properties of the trichotomous noise, the analytical expression of the output amplitude gain (AG) is derived. It is shown that the AG is a non-monotonic function (NF) of the frequency of the input signal, an NF of the values of the capacitance and resistance, as well as an NF of the amplitude and flatness of the trichotomous noise. The cut-off frequency and band-width of the system becomes larger than those of the noise-free system.

    • >Physics
    • The comparison of several time phase unwrapping methods

      2016, 53(1):110-117.

      Abstract (2468) HTML (0) PDF 650.83 K (2287) Comment (0) Favorites

      Abstract:Abstract: One of the key steps in 3D optical shape measurement based on the structured light projection is the phase unwrapping. The unwrapping issue about the complicated phase map is also the difficult step of the methods. The temporal phase unwrapping provide a useful tool to obtain the continuous phase map from the complicated wrapped phase map. Along the time direction, the temporal phase unwrapping is independently carried out from a 3D distribution demodulated from grating fringes with different pitches projected on the tested object. Therefore the problem of the error propagation in 2D phase unwrapping algorithm can be avoided. Followed by the comparison among the typical temporal phase unwrapping methods, including the negative exponential method, three pitches unwrapping method, three pitches heterodyne unwrapping method and its improved technique, a segmentation curve fitting method is used to improves the phase calculation accuracy. The computer simulations and experiments are carried out to verify that the phase error is deduced greatly through the segmentation curve fitting.

    • Nuclear coupling parameters affect hyperon phase in the neutron star

      2016, 53(1):118-123.

      Abstract (2065) HTML (0) PDF 650.83 K (985) Comment (0) Favorites

      Abstract:Hyperon emergence and the proportion of hyperon have significant influence on the properties of the neutron star, How the different nuclear interaction models affect the neutron star is an important physical problems. In terms of the relativistic mean field theory, selecting different nucleon coupling parameters of neutron stars to calculate the density of hyperons. And we will find that the different nucleon coupling parameters have different extent influence in hyperon emergence and the transition of neutron star to hyperon star. The interaction potential of sigma meson and omega meson potential have a significant influence, but the influence of rho meson potential is small. These results have important reference value in researching the massive neutron star.

    • Pathology of the Fermionic Representation of Transverse Ising Model

      2016, 53(1):124-130.

      Abstract (2131) HTML (0) PDF 760.65 K (1097) Comment (0) Favorites

      Abstract:When studying the quantum Ising chain in a transverse field, we usually transform the spin operators to fermion operators by applying the Jordan-Wigner transformation,i.e.we take it for granted that the spin model and corresponding fermionic model are equivalent naively. But deliberate treatment could reveal that the equivalence accompanies some delicate conditions.Redundant degrees of freedom should be projected out carefully, especially for finite length of chain. In this paper, we compare the two models in detail so as to find the conditions of equivalence of them,including the interaction, the boundary conditions, and odevity of the number of lattice sites.We also exemplify in detail how to project out the redundant degrees of freedom for the fermionic representation.

    • Effect of many-body interactions on the equation of state for Solid Argon

      2016, 53(1):131-137.

      Abstract (2139) HTML (0) PDF 1.54 M (1435) Comment (0) Favorites

      Abstract:By using ab initio self-consistent field Hartree-Fork methods and atomic clusters expanding theory and employing Gamess program, two- and three-body interaction energies and the equation of state for solid argon in fcc crystal have been quantificationally calculated at the neighboring atomic distance R=2.40 -4.00 Å under high pressure. In addition, the convergence and truncation of atomic energy multi-body expansion are analyzed. It is found that two-body contribution to the cohesive energy is positive, while the three-body contribution is negative in solid argon under high pressure. At high pressure, only the consideration of the two-body contribution will overestimate the cohesive energy, hence it is necessary to introduce the three-body negative effects. The phenomenon of the experiment under 80GPa can be explained by considering the three-body potential.

    • >Chemistry
    • Structure and Spectral Properties of HgmSen(m=1,2,3,n=1,2) Molecular and Molecular Ion lusters

      2016, 53(1):138-144.

      Abstract (2051) HTML (0) PDF 1.41 M (1281) Comment (0) Favorites

      Abstract:This paper adopted the LANL2DZ pseudopotential basis set and B3LYP method to calculate the microstructure and electronic properties of HgmSen(m=1,2,3,n=1,2) molecules and molecular ion clusters. Meanwhile, it utilized the time-dependent density functional theory to calculate the absorption spectra, thus obtaining the ground state structure, electronic properties, binding energy, energy gap, dipole moments and other microscopic properties. Then it analyzed the frontier molecular orbital characteristics, identified all the harmonic vibration mode and absorption spectroscopic properties. The results show that: when m+n=1~3, the linear and angular types are the stable geometry with ground state; when m + n = 4 ~ 5, the ring type is the stable geometry with ground state, besides, compared with other molecular clusters, HgSe2 molecular cluster have the greatest stability, binding energy and the minimal energy gap. HgmSen(m=1,2,3,n=1,2) molecules and molecular ion clusters have better electron donors, receptors and other active sites, that is, great electrical conductivity. The maximum absorption wavelength λmax of each cluster with different composition lacks of regularity, but for the molecular clusters with the same composition and its corresponding positive and negative ions, there appears significant red shift or blue shift. The results could provide theoretical reference for the further experimental and applied research, and it is conducive to studying the nature of experiments as well as certain laws of massive condensed matter.

    • Synthesis and Characterization of Micro- and Nano- Metal-porphyrin Coordination Polymer

      2016, 53(1):145-151.

      Abstract (2056) HTML (0) PDF 1.32 M (1548) Comment (0) Favorites

      Abstract:Micro- and nano-scale metal-organic complexs not only have size effect, but have an adjustable assembly unit, which shows a lot of good performance. in this paper, we synthesized nanoscale mertal-porphyrin complexs by a simple and green method. by choosing the different metal ions, the different nanostructures (0D, 1D, 2D) of metal-porphyrin polymer was abtained by controlled assemble unit. its structures were characterizatized by scanning electron microscopy (SEM), infrared spectroscopy (IR), x-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), and were detailed examinated of the impact and dynamica growth of the metal-porphyrin polymer.

    • >Physics
    • Preparation and luminescent of Zn1-xMnxS nanoparticles

      2016, 53(1):152-156.

      Abstract (2110) HTML (0) PDF 1.08 M (1598) Comment (0) Favorites

      Abstract:Mn-doped ZnS (ZnS:Mn) nanoparticles were prepared via hydrothermal method, studied effects on photoluminescence that different proportions Mn ions on Zn1-xMnxS. The composition, morphology, microstructure and optical property of the sample were characterized by X-ray diffraction (XRD).The results showed that the Zn1-xMnxS (x = 0,0.01,0.03,0.05,0.07,0.10) nanoparticles were spherical hexagonal structure of nanocrystalline, uniform particle size between 15 ~ 20nm. At room temperature, using the UV excitation wavelength is 350nm stimulate Zn1-xMnxS nanoparticles. Blue light emission which is produced by transition between shallow donor level and manganese t2 level can be obtained. Luminous intensity with the increasing amount of manganese-doped increased decreased after the first.And emission peaks have a mobile with the change of manganese-doped content. The reason may be related to sulfur vacancies which influence shallow donor level of ZnS. The proportion of zinc-manganese has an impact on the amount of sulfur vacancies and level position.

    • >Material Science
    • Preparation of the high-quality and stable

      2016, 53(1):157-162.

      Abstract (1925) HTML (0) PDF 1.94 M (945) Comment (0) Favorites

      Abstract:High-quality microcrystalline silicon films with improved stability were prepared by plasma enhanced chemical vapour deposition technique from SiCl4/H2 under 250℃, at a higher rate over 0.28nm/s, with a crystalline fraction of 80%. The photoconductivity of the microcrystalline silicon films keeps a constance after 540min long light soaking. The thickness uniformity of films was markedly improved (more than 95%) by altering the distribution of pore.

    • >Biology
    • Expression and Purification of Recombinant Chicken TSHβ, FSHβ, and LHβ Proteins

      2016, 53(1):163-168.

      Abstract (2251) HTML (0) PDF 1021.18 K (1905) Comment (0) Favorites

      Abstract:Gonadotropin(Follicle-stimulating hormone (FSH), Luteinizing hormone (LH))and Thyroid-stimulating hormone (TSH) are glycoprotein secreted by pituitary. Gonadotropin plays a key role in the regulation of the development and function of vertebrate gonads. TSH participates in development and metabolism by stimulate the thyroid to secrete T4 and T3. In the study, we prepared the prokaryotic expression plasmids encoding chicken TSHβ subunit, FSHβ subunit and LHβ subunit and produced the three recombinant proteins using RT-PCR and molecular approaches. We also purified the recombinant proteins by Metal Ion Affinity Chromatography. This work paves the way for future preparation of polyclonal antibodies against chicken TSHβ, FSHβ and LHβ in rabbits.

    • HPLC determination of activity of UGT75B1、UGT71B6、UGT71C5 and the kinetic analysis

      2016, 53(1):169-174.

      Abstract (2063) HTML (0) PDF 1.62 M (1535) Comment (0) Favorites

      Abstract:The cDNA sequences of UGT75B1, UGT71B6, UGT71C5 were cloned by PCR method and then were constructed into glutathione S-transferase gene fusion vector PGEX-6P-1. All of the plasmids were transformed into E. coli strain BL21 individually for recombinant protein expression. Recombinant UGTs were purified from supernatant of cell lysates. To investigate the activity of recombinant UGTs, HPLC was employed to determine their activity to catalyze ABA and p-aminobenzoic acid. Analysis by reverse-phase HPLC was carried out using a Kromasil C18 column (250mm×4.6mm, 5μm) and glucose ester was separated by a linear gradient of 10%-100% methanol in H2O (all solutions contained 2.5ml/L of acetic acid and 0.4ml/L of triethylamine) or 10%-20% acetonitrile in H2O (all solutions contained 1ml/L of trifluoroacetic acid). The results showed that only UGT75B1 presented strong p-aminobenzoic acid acylglucosyltransferase activity and the others had lower activity. All of the recombinant UGTs had ABA-GE activity. However, UGT71B6 performed highest activity. Analysis of Michaelis-Menten kinetics of UGT75B1, UGT71B6 and UGT71C5 indicated that their Km was 0.73, 0.43 and 0.45 mM, respectively. In conclusion, our investigations demonstrate that UGT75B1 has higher p-aminobenzoic acid acylglucosyltransferase activity than ABA-GE activity. It seems that p-aminobenzoic acid is the specific substrate of UGT75B1. In comparison, UGT71B6 and UGT71C5 with similar Km perform higher ABA-GE activity.

    • Extraction and physicochemical properties of Idesia polycarpa Maxim oil

      2016, 53(1):181-186.

      Abstract (1991) HTML (0) PDF 1.15 M (918) Comment (0) Favorites

      Abstract:In this study, extraction method and decolorization as well as grease physical of the oil from the fruit of Idesia palycarpa were analyzed. The chemical compounds of the oil were separated and indentified by capillary GC-MS method. Meanwhile, the content of polyphenols from Idesia palycarpa oil were determined to provide the basis for the future development of the Idesia polycarpa Maxim. The results showed that the extraction rate by Soxhlet extraction was higher than the traditional press law, extraction rate reached 35%, while the traditional press law was 21%. Among diethyl ether, petroleum ether and n-hexane, the extraction rate by using petroleum ether with Soxhlet extraction was the highest. Through the extraction of oil, oil content of Idesia polycarpa was about 35%. Results also indicated that decolorization rate increased with the rise of the temperature, it has reached 45% while bleaching temperature up to 80℃. The main components of the oil were palmitoleic acid, palmitic acid, linoleic acid, oleic acid and stearic acid, the content of linoleic acid was the highest, up to 60%. In general, Idesia palycarpa has high oil content, and rich in linoleic acid in the oil, the results showed that Idesia palycarpa oil has considerable prospects for the development.

    • Cloning and expression regulation analysis of a JcKNOX1 gene, from Jatropha curcas L.

      2016, 53(1):187-192.

      Abstract (2051) HTML (0) PDF 2.71 M (880) Comment (0) Favorites

      Abstract:KNOX family genes,coding homeobox proteins,play an inmportant role in plant development.A novel homeobox gene,JcKNOX1,was cloned from the cDNA of Jatropha curcas L..The full-length ORF of JcKNOX1 was 693bp,encoding a protein of 230 amino acids residures.The predicted structure of JcKNOX1 showed that its theoretical isoelectric point and molecular weight were 6.13 and 25.92 kDa respectively.Phylogenic analysis indicated that JcKNOX1 was relation to Populus tomentosa and Citrus sinensis closely.By fluorescence quantitative PCR the expression levels were different as follows:stem apex>tender leaf>stem>leaf>flower>petiole>root,which showed that the JcKNOX1 expressed with high levels in developing organs.Meanwhile,JcKNOX was strongly induced by ABA and low temperature in Jatropha curcas L. seedlings.Through biology software prediction analysis indicated that JcKNOX1 might play an important role in light regulation,hormone regulation,expression of pollen-specific gene and abiotic stress of Jatropha curcas L..

    • Synthesis and evaluation of near infrared fluorescent dye BPAD-2 for β-amyloid plaques

      2016, 53(1):193-197.

      Abstract (2000) HTML (0) PDF 1.21 M (1082) Comment (0) Favorites

      Abstract:OBJECTIVE We synthesized a benzopyrane derivative BPAD-2 with push-pull electronic structure, and assessed its fluorescence properties, labelling ability for beta-amyloid plaques, and blood-brain barrier penetrating ability. METHODS BPAD-2 was synthesized by condensation reaction with 1-(2-hydroxy-phenyl)-ethanone as the primary reagent, and confirmed by 1H-NMR and MS. Fluorescence spectra were observed; fluorescence staining was performed to evaluate labelling ability for beta-amyloid plaques; fluorescent imaging was performed to evaluate blood-brain barrier penetrating ability. RESULTS The emission maximum of BPAD-2 in PBS was at 658 nm; BPAD-2 showed bright red labelling of beta-amyloid plaques; BPAD-2 penetrated the blood-brain barrier and peaked at 10 min postinjection and showed reasonable washout from the brain. CONCLSION BPAD-2 showed specific labelling of beta-amyloid plaques and had good blood-brain barrier penetrating ability.

    • Human elongation factor 1A1(eEF1A1) promotes drug tolerance in lung cancer cell line

      2016, 53(1):198-202.

      Abstract (2162) HTML (0) PDF 910.17 K (1388) Comment (0) Favorites

      Abstract:Human translational elongation factor 1A (eEF1A)is a multi-functional protein besides its roles in protein translation system. The protein expressional level of eEF1A is related to cell viability in oxidative stress and apoptotic process. Based on the knowledge of multifunctional roles of eEF1A and its relation to cell viability, we studied the possible effect of the isoform eEF1A1 on the drug resistance in cancer cell lines. The eukaryotic expression vector containing eEF1A1 sequence was constructed and was transiently transfected into human lung cancer cell line H1299. After verifying the expression of FLAG -eEF1A1, MTT assay was utilized to measure the cell viability of transfected H1299 after treatment by two common anti-cancer drugs. H1299 transfected with eEF1A1 showed enhanced drug tolerance to both taxol and adriamycin. Lung cancer cell highly expressing EEF1A1 behave more tolerance to antineoplastic drugs.

    • Molecular Cloning and Expression Patterns of LcAO from Lycium Chinens

      2016, 53(1):203-208.

      Abstract (1923) HTML (0) PDF 1.02 M (1222) Comment (0) Favorites

      Abstract:A ascorbate oxidase (AO) gene from Lycium Chinense was cloned with homologous method. The full-length cDNA of Lycium Chinense AO which was named LcAO (Genbank: KP712033) was 1737 bp,and contained a complete open reading frame (ORF) of 578 amino acid residues. Homology analysis indicated that the deduced LcAO protein was highly 90% to AO proteins from Solanum lycopersicum. The results from Real-Time PCR indicated that the expression of LcAO gene was the strongest in the flower and friut, and the least in mature-leaf of Lycium Chinense.

    • Construction and Application of RecET Recombination System in Zymomonas mobilis

      2016, 53(1):209-214.

      Abstract (2260) HTML (0) PDF 1.00 M (1807) Comment (0) Favorites

      Abstract:Zymomonas mobilis is a Gram-negative bacterium with excellent ethanol-producing capabilities. The ability of ethanol yield and substrate utilization can be further improved through genetic manipulations. In this study, in order to increase the recombination efficiency of foreign genes into chromosome of Z. mobilis, the RecET genes were cloned into the E. coli-Z. mobilis shuttle expression vector pSUZM3a, resulted in pSUZM3a-RecET. The adhA gene encoding the alcohol dehydrogenase and tetracycline resistant gene were used as the target and the selection marker genes, respectively. The PCR fragments of tetracycline resistant marker with 60bp flanking sequences homologous to adhA were electroporated directly into Z. mobilis ZM4 cells which harbored pSUZM3a-RecET. After the PCR analysis and DNA sequencing, it was found that the RecET-mediated recombination reaction resulted in adhA gene replaced by tetracycline resistant gene. The result showed that RecET system could make efficient, rapid targeted gene knock-out with only 60bp homologous arm in Z. mobilis.

    • Screening of Phospholipase D producing Strains and its transphosphatidylation activity

      2016, 53(1):215-220.

      Abstract (2137) HTML (0) PDF 1.16 M (1826) Comment (0) Favorites

      Abstract:Using soybean Phospholipids as substrates,one strain which expressed Phospholipase was isolated from soil near oil mill ,the Phospholipase produce by SNUPLD-6 was identified which contain Phospholipase D . According to its mor -phological characteristics and ITS sequence , The strain SNUPLD-6 was identified as Geotrichum candidum. study on the Conditions for the growth of the bacteria and the PLD reaction conditions which expressed by SNUPLD-6. The results showed that its optimum growthtemperature is 30℃ .its optimum growth pH value is 6.0,its optimum carbon source is glucose,its optimum nitrogen source for the beef extract and peptone 50% each. the optimal fermentation time of phospholipase D producing is 3d, its optimum transphosphatidylation-temperature is 32℃. its optimum transphosphatidylation pH value is 6.0,its optimum reaction buffer for pH=5.5 0.02M HAc -NaAC buffer, its Optimal metalion activator as Ca2 +, Zn2 +.

    • Study on the application of aquatic plants in Chengdu Egret Wetland Park

      2016, 53(1):221-227.

      Abstract (2000) HTML (0) PDF 1.25 M (1328) Comment (0) Favorites

      Abstract:Abstract: Based on field survey and analysis of data, the application status of aquatic plants in Chengdu Egret Wetland Park was studied, including the contents of species selection, planting modes, landscape effects and ecological functions, the monitoring and analysis of ambient air quality and surface water quality were also conducted. And then the advices were put forward for the further strengthening of application and growing management about aquatic plants in Chengdu Egret Wetland Park. The study provided a reference for the construction of ecological zones around the city of Chengdu in the future about the rational use of aquatic plants.

    • TAp73α-mediated cisplatin sensitivity was suppressed by miR-330 in colorectal cancer cell HCT116

      2016, 53(1):228-234.

      Abstract (2175) HTML (0) PDF 2.36 M (1126) Comment (0) Favorites

      Abstract:The p53 family member p73 is a transcription factor involved in the regulation of cell cycle, apoptosis and cancer cell drug-resistant. Here we reported that knockdown of p73 sensitized HCT116 colorectal cancer cells to cisplatin treatment independent of p53. Hsa-miR-330 (miR-330) was identified to be able to directly repress the expression of TAp73α. Overexpression of miR-330 decreased the protein levels of endogenous TAp73α and phenocopied the effect of p73 knockdown. Restoration of TAp73α eliminated miR-330-induced chemosensitivity toward cisplatin. Our results demonstrated a novel function of TAp73α to impair cisplatin sensitivity in colorectal cancer cells which can be repressed by miR-330, thus provided an effective strategy for therapeutic treatment of cisplatin-resistant cancer cells.