Abstract:In this paper, we consider the relationship between the Deligne-Simpson problem and the Hurwitz enumeration problem. First, we observe that they are the solutions of the same algebraic equation on different groups, which the algebraic equation is (A_1,B_1)…(A_g,B_g)X_1…X_k=I. When G is the general linear group over the complex field, this equation is equivalent to Deligne-Simpson problem; When G is the general linear group over the finite field, this equation is equivalent to the Euler characteristic of solution space for Deligne-Simpson problem; When G is the permutation group, the equation is equivalent to the Hurwitz enumeration problem. Then we calculate the Euler characteristic of the 3th order Deligne-Simpson problem with any partition, and we express the generating function of some Euler characteristic as the rational functions.

Abstract:It is well known that normal bases over finite fields have been implemented efficiently in software. The hardware and time complexity of multiplication using normal bases depends on the structure of the normal basis used, particularly on the complexity of the normal basis. Therefore to determine the complexity for normal bases, especially Gauss normal bases over finite fields, is interesting. By properties for finite fields and elementary techniques, we obtain the upper and lower bounds of the complexity for the dual basis of a class of the type (n,k)(k\geq 3) Gauss normal bases, and determine the explicit complexity of the dual basis for the type (n,k)(k=1,2) Gauss normal bases over finite fields, which is an elementary proof for the main results given by Wan and Zhou in 2007.

Abstract:The numerical solution of coupled Darcy-Stokes flow problem is studied in this paper. The coupled Darcy-Stokes flow models are composed by Stokes equation in the flow domain and by Darcy's law in porous medium domain, and by proper interface conditions between the two domains. By introduced Lagrange multipliers to process interface, New variational form for coupled Darcy-Stokes model is proposed. The problem is discreted by the low order conforming H(div) R-T elements . Lastly, we prove the existence and uniqueness of the discrete problem and get error estimates.

Abstract:This paper focuses on multistable analysis of one dimensional Hopfield neural networks, whose sigmoid activation function may not be bounded. Firstly, the condition for the existence of equilibria is established. Moreover, the conditions for exactly 1, 2, or 3 equilibria and their stability respectively are proposed with some constraints for network parameters. Then, the corresponding results about the equilibria features are supplemented in the remaining cases for the parameters. Thus, we obtain the relationships between all the different values of the parameters and the number of equilibria as well as their stability. Finally, by employing bounded and unbounded activation functions, two examples and numerical simulations are used to illustrate the theory developed in this paper.

Abstract:The numerical solution for an homogeneous boundary conditions of generalized Rosenau - Kawahara equation is considered.A nonlinear two-level Crank-Nicolson difference scheme is designed.The difference scheme simulates the conservation properties of the problem well.The prior estimate, existence and uniqueness of the finite difference solution are also obtained. It is proved that the finite difference scheme is convergent with second-order and unconditionally stable by discrete functional analysis method.The numerical example show this scheme is feasible.

Abstract:Wheat stripe rust prediction has very vital significance for the prevention and control of wheat disaster.This paper forecast the wheat stripe rust incidence in Chengdu using PSR and LSSVM.The Meteorological data was as input parameters, and the incidence rate was as the output parameter in the test. To predict the 20 cases samples ,this paper got better predictive effect.The method in this paper is much better than SVM, through the comparative analysis.So,the model Is very effective to For wheat stripe rust prediction.

Abstract:Sophie Frisch characterized the structure of the group of polynomial permutations over $\mathbb{Z}/p^2\mathbb{Z}$. Qifan Zhang found a correspondence between polynomial functions over $\mathbb{Z}/p^2\mathbb{Z}$ and 3-tuples of polynomial functions over $\mathbb{Z}/p\mathbb{Z}$, this paper is giving another proof of [1]. In this paper, we first prove that over any finite commutative ring $R$, the group of polynomial permutations is isomorphic to the automorphism group of the $R$-algebra of the polynomial functions. Then we give an easy proof to the characterization of Sophie Frisch using the correspondence set found by Zhang.

Abstract:In this paper, we discuss a retarded nonlinear integral inequality with two variables. By using the techniques of monotonization and reducing the function outside the integral to constant, we give an estimation of the unknown function in the inequality. Finally, by applying our result, we prove that the solutions of two types of equations, one are integral equations and the other differential equations, are bounded.

Abstract:Efficiently and realistic rendering of large-scale has always been a thorny issue in graphics rendering field, especially when kernel memory can’t hold the entire scene at once. Bounding volume hierarchies is a kind of object-based scene management techniques which is widely used in collision detection, ray tracing system, etc. This paper focuses on large-scale scene and proposes a novel algorithm which named Multi-Level Bounding volume hierarchies, at last implementing it using GPU parallel computing power. Our algorithm has some contributions for large-scale scene. The first uses a linear ordering derived from spatial Morton codes to chip scene data into block extremely quickly and with high parallel scalability; the second is that the traverse efficient have significantly improved by using a two phase traverse tactics, which using the first stage result to control the second stage traverse. Finally, we have done some experiments to proof the algorithm correctness and reliability, also have analyzed the construction and traverse efficiency.

Abstract:In Mobile ad-hoc network (MANETs) field, with the moving characteristic of nodes leading to the dynamic change in network topology, security patches can't rapidly spread in the network, thus not to study the virus propagation immuning to the Mobile ad-hoc network pulse. Considering the realistic feasibility of MANETs pulse immunization, MANETs virus transmition pulse immunization model was established. Based on the Impulsive Differential equation (Impulsive Differential Equations, IDE) stability theory, the MANETs virus propagation behavior under pulsed immune and the critical characteristics of infection denoting whether viruses die out were analyzed, as well as the existence, stablity of the system periodic solution of disease-free (diseases - free, DF), and the persistent existence of the Endemic diseases(Endemic, ED). With corresponding parameters selected, the numerical simulation was carried out upon the system, the experimental results of which validate the correctness and consistency of the conclusion .

Abstract:In order to effectively improve the identification ability for fault data of wireless sensor network, a novel mining algorithm FDMBFO (Fault Data Mining algorithm based on Bacteria Foraging Optimization) is proposed by bacteria foraging optimization. In this algorithm, the division method of distribution range is given with wavelet transform and correlation coefficient, and the objective mining function is built. Then, the solving of function is presented by bacteria foraging optimization. Finally, a simulation with actual sample data was conducted to study the key factors of FDMBFO. Compared to performance of other algorithm, the results show that, FDMBFO has better adaptability.

Abstract:Flight scheduling has been a complex and key task for the air traffic control (ATC), and aircraft landing scheduling (ALS) problem is one of the core issues. ALS is a NP-hard problem with a large scale and multi-constraints characteristics. Thus, in order to solve the flight landing problem effectively and rationally, a flight landing scheduling algorithm based on receding horizon and genetic-immune algorithm (RHC_HGIA) is proposed. RHC_HGIA solves the problem of flight landing by two aspects mainly, one is that selecting the flights that are waiting to land and need to be optimized based on the receding horizon length and size which have been set; on the other hand, optimizing The selected flights which are waiting to land by using genetic-immune algorithm and determining actual landing time of them. the flights that have been optimized form a new flight landing sequence, selecting the flights from the sequence that the actual landing time of them in the field within a given time range to land. Then resetting receding horizon length and re-selecting the flights to be optimized until all pending landings have landed so far. In this paper, simulation is conducted in the airport control simulation system on the base of an airport of 20 flights to be landing of one day. Simulation results show that, RHC_HGIA algorithm can solve ALS problem preferably, and comparing with traditional flights landing scheduling algorithm(FCFS), the extra costs of flight is reduced much more.

Abstract:Runway capacity is an important indicator of the level of airport services. Because of simple runway taxiway structure in small and medium-sized aviation airport, runway occupancy time is longer while aircraft taking off and landing is the main factor affecting airport capacity. Detailed aircraft landing, taxiing time and introduced of regulatory rules, the capacity evaluation model considering runway and taxiway structure was proposed. Programming with Visual C++ for numerical simulation, the results show that the model quantified the key factors acting the airport runway capacity and runway occupancy time, such as real rolling distance, taxiway location, taking off and landing proportion In Ganzhou airport, for example, the model of capacity evaluation results accord with actual flight principles and control rules, to improve the airport throughput to provide technical support, has certain reference value and practical.

Abstract:A superstrate is able to significantly increase the gain of an antenna, and this superstrate based technology has been widely used. However, the studies about the effects of superstrates on the return loss of the antenna are limited. In this work, taking homogenous dielectric superstrates and a probe-fed microstrip antenna as examples, using numerical simulations, the effect of superstrates on the return loss of an antenna is investigated. The simulation results show, by proper design of some structural parameters, such as the distance between the superstrate and the antenna, the feeding location of the antenna and so on, the dielectric superstrate can improve both the gain and the impedance bandwidth. A prototype is fabricated and measured, and the measured results shows that it has a gain of 14.5 dBi and a |S11|<-10dB impedance bandwidth of 11.19% , which verifies the simulations.

Abstract:The stochastic resonance (SR) in a semiconductor layer driven by a dichotomous noise and square-wave signal is investigated. On the assumption that the system satisfies the adiabatic approximation condition, applying the two-state theory, the expression for the output signal-to -noise ratio (SNR) of the system is obtained. The non-monotonic influence of the surrounding temperature on the SNR is found. It is shown that the SNR is a non-monotonic function of the standard deviation of the impurities’ position and the system bias: By choosing the deviation and bias of the impurity, the system SNR can be tuned. For low temperature, large bias can improve the system SNR. Moreover, the SNR increases with the amplitude of the square-wave signal and the ratio between temperature of the hot and cold reservoirs for relatively high temperature, while the SNR decrease with the increase of potential energy and the strength of the dichotomous noise. The results obtained in this paper have certain theoretical significance for the semiconductor design and the investigation of the semiconductor layer process.

Abstract:Based on the SNESIM algorithm, a fast three-dimensional(3D) reconstruction algorithm of multiple-point statistics simulation was proposed. First, pattern sets were built by the red-black tree. The height of tree structures was rapidly reduced. CPU time for retrieving matching data events was saved. Then, the algorithm combined the advantages of probability aggregation approach and the sequential two-dimensional(2D) simulations with sample data approach. Half-template and all-template were utilized in nodal simulation. The proportion of informed conditioning nodes in template was increased so that the amount of possible data event was declined. The algorithm was tested on 2D channelized reservoir section, 3D porous medium images and 3D reservoir rock images. The results showed that this algorithm achieved the efficiency without degradation of accuracy. No matter isotropic or anisotropic rocks, the visual characteristics, the statistics characteristics and the pores-throats structures of the reconstructions were similar to the corresponding real 3D digital rock images, which proved that this algorithm was reliability.

Abstract:In this paper, the electromagnetic environment (EME) in a campus is measured and then analyzed adopting statistical method. The results establish the spatial distribution of the EME in this campus, and the characteristics of EME in different areas are compared. The probability density functions (PDF) of the measured data are calculated and depicted, and some other valuable results are also obtained. These measured data and statistical results provide necessary support for evaluating and analyzing the EME. And these data could be utilized to affirm theoretical analysis method of EME. Finally, this paper presents a method that using Correlation to represent the complexity of EME. Two electromagnetic field intensity probes are used to sample the electric field strength of different positions simultaneously, the Correlation Coefficients between two electric field strength versus two different time series are calculated to measure the complexity of EME, and the figures of correlation coefficient corresponding to each component and total field versus distances are presented.

Abstract:In this paper, we investigated operational characteristics and approximation performance of Oustaloup fractance rational approximation from a new perspective of fractional calculus operation. Based on order-frequency characteristic and phase-frequency characteristic theoretical analysis, we start from the operational characteristics of pole-zero sub-systems, by the pole-zero recursive distribution of which, we study operational characteristics of Oustaloup fractance, and in order to analyze its operational characteristics and approximation results, relative error function，approximation bandwidth, K-index, complexity and approximation effect were used. Theoretical results showed that fractional order-frequency characteristics can analyse Oustaloup fractance rational approximation simply and exactly, the fractance rational approximation has faster approximation speed and lower complexity. Providing a solid foundation for the application of Oustaloup fractance circuit, to provide a theoretical basis for the design of fractional controller.

Abstract:Carrier-type conversion between p- and n-Ba8Ga16Ge30 (BGG) single crystals was realized. The temperature dependence of resistivity has been measured to judge the carrier types of the new batch samples synthesized using physical property measurement system (PPMS). The resistivity data show that p- and n-type BGG could be converted to each other under the addition of excessive components. This approach is instructive for tuning the charge carrier type or concentration to effectively investigate the vibration behavior of guest atoms and host cages. In addition, it can also provide some important information to the recycle of the thermoelectric materials.

Abstract:The geometry of TPPFe(II) and L-Thr-TPPFe(II) were optimized using density functional theory (DFT) at the B3LYP level with the mixed basis sets: Lan2dz for Fe(II), 6-31G(d) for C and H atoms, and 6-311++G(2d, p) for N and O atoms. To simulate real conditions, the solvent effects of CHCl3 and H2O were studied using the polarized continuum model (PCM). The electron density at the bond critical point was calculated using the topology analysis of the Multiwfn software. Based on the calculated data of electrostatic potential obtained by quantitative analysis of molecular surface using the Multiwfn software, the molecular electrostatic potential maps were illustrated using VMD software. The bond length and electron density at the bond critical point indicate the coordination bond between the Fe(II) atom and the O atom of carbonyl group in L-threonine and the intramolecular hydrogen bond between H(7) atom and N(1) atom. The porphin ring is warped by the effect of the coordination bond and the intramolecular hydrogen bond. The coordination effect between the Fe(II) atom and carbonyl group may weaken the bond strength between Fe(II) atom and O2, but it is beneficial for the reversible oxygen carrying functions. The solvent effects of CHCl3 and H2O may attenuate the intramolecular hydrogen bond and the coordination effect between the Fe(II) atom and carbonyl group, and enhance the warping of porphin ring and the coordination effect between Fe(II) atom and O2. The computation of molecular electrostatic potential show that the more negative value outside the O atom, N atom and benzene ring are contributed by lone-pair electrons and π-electrons, respectively. The electrostatic potential outside the H atom of the benzene ring and the Fe(II) atom is positive. The most positive value is outside the Fe(II) atom which is the most probable active sites in coordination reactions between the Fe(II) atom and O2.

Abstract:We investigate the stability of the localization of a Bose-Einstein condensate in one-dimensional, two-dimensional and three-dimensional bichromatic optical lattices using a time-dependent variational approach. We derive the effective potential for the stability analysis of the system using the Gauss type trial wave function and give the stable criteria through this effective potential. It is demonstrated that the bichromatic optical lattice's intensities, two-body, three-body and high-order interactions play different roles in the stability of the system. The two-body and high-order have an important influence to the stability but the others play a regulatory role on the stability.

Abstract:It’s important to understand the deformation behavior of metals in the dynamic loading process. In this study, the dynamic mechanical responses of high strength steel are investigated by uniaxial compressive experiments on the Split Hopkinson Pressure Bar (SHPB) with strain rate range from 3000 to 12000s-1 and temperature range from 20℃to 800℃. The experimental results show that the mechanical responses of steel investigated are strongly affected by the strain, strain rate and deformation temperature. The flow stress increases with the increasing of strain rate while decreases with the increasing of deformation temperature. A phenomenological constitutive model is established to describe the strain rate harden and temperature soften behavior. The stress-strain relationships predicted by the phenomenological constitutive model agree well with the experimental results, which shows that the proposed constitutive model can be used to study the dynamic deformation process furthermore.

Abstract:The molecular structures, spectral and thermodynamic properties of Dual Prenylated Flavonoids were calculated using the Density Functional Theory B3LYP method. Then, the polarized continuum models(PCM) of Tomasi were used to discuss solvent effects. The computational results showed that the lowest energy absorption wave length were 325.6 and 361.9 nm for 1R and 2 molecules in gas, and the solvents and polarity have few influence on the lowest energy absorption wave length. At 298 K and standard pressure, the thermodynamic properties ΔfHm of 1R and 2 molecules were 3455.51 and 3691.26 kJ/mol, respectively, and thermodynamic properties ΔfGm of 1R and 2 molecules were 4028.23 and 4282.98 kJ/mol, respectively, and Sm of 1R and 2 molecules were 834.90 and 834.97 J.mol-1.K-1, respectively.

Abstract:The interaction of 1,4,7-Triazacyclononan -Co(Ⅱ) (Cyclen-Co(Ⅱ))with bovine serum albumin (BSA) has been investigated by fluorescence method. The results show that the Cyclen-Co(Ⅱ) strongly quenched fluorescence of BSA. It was proved that the fluorescence quenching mechanism belonged to the static quenching by Stern-Volmer equation. According to Lineweaver-Burk equation and thermodynamics equation, the binding constants,binding sates and thermodynamics parameters were obtained at different temperatures in the aqueous solution and in the solutions of Brij35、CTAB、SDS. The hydrophobic interaction forces played a main role in the binding of Cyclen-Co(Ⅱ) with BSA.

Abstract:Based on aqueous two-phase system of polyethylene glycol (PEG)/salt, a simple pretreatment approach was developed for the extraction and determination of salidroside in Rhodiola. The influence factors on phase behavior of forming biphasic and partition behavior of the ATPS was investigated. Studied the factors such as polyethylene glycol (PEG) molecular weight and the mass fraction, the kinds of salt, the salt mass fraction, ionic strength, salidroside initial concentration influence on salidroside extraction rate. Determine the optimum extraction conditions with orthogonal experiment, when the aqueous two-phase system is 20%PEG1000, 20% (NH4)2SO4 and 1% KCl, the extracting effect is best; the extracting rate is 95.32%.Compared with the conventional liquid–liquid extraction or solid-phase extraction, this method was more environmentally, more cost effective.

Abstract:Tantalum electrolytic capacitor anode was prepared by electrochemical method. The surface structure characteristics and formation mechanism Tantalum electrolytic capacitor anode was prepared by electrochemical method. The surface structure characteristics and formation mechanism of tantalum anode section were studied by field emission scanning electron microscopy (SEM) and theoretical analysis. The results show that a gap layer (<1nm), caused by oxygen vacancy and its defect ion migration, is embedded in the Ta/Ta2O5 film curve surface. Moreover, surface film of the interface structure stress model shows that the defect concentration produced in the surface electrochemical growth process is higher than that of planar system. The mechanism of the electric field stress distortion shielding related to the formation process of the tantalum electrolytic capacitor film is discussed.

Abstract:The total proteins extracted from a radiation-resistant bacterium Micrococcus luteus SC1204 were separated by immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE), and ImageMaster 2D Platinum 5.0 was applied to analyse 2-DE images after silver staining. The 2-DE was optimized by comparative tests on the important factors including extraction methods, lysis buffer components, pH range of IPG strips, sample volume, and isoelectric focusing time. The results showed that the resolution of 2-DE profiles was significantly improved by liquid nitrogen grinding-phenol∕ultracentrifugation in lysis buffer Ⅱ for sample preparation, pH 4-7 (24 cm) IPG gel strips, the sample loading at 250 μg, and prolonged isoelectric focusing time (56000 vhr). This work provided a technical basis for the further study on differential proteomics in M. luteus SC1204.

Abstract:Retinoblastoma tumor-suppressor proteins (Rb) plays a vital role in regulation of cell proliferation and tumor development. Recent research reveals that peptidyl-prolyl isomerase (Pin1) can regulate the function of Rb. In this study, we examined the cellular localization of Pin1 in tumor tissues, and investigated the effects of Pin1 localization on Rb phosphorylation. Our data demonstrated that knockdown of Pin1 result in a decrease of Rb phosphorylation and cellular viability. Pin1 can localize to cytoplasm in cultured tumor cells and tumor tissues, while cytoplasmic Pin1 can increase the phosphorylation of Rb. This is a novel mechanism for Pin1 in regulating Rb function.

Abstract:To construct miR-122 overexpression Adenovirus vector，gene including eight miR-122 precursor fragments was inserted into recombinant construct pDC312-cmv. Abilities of the recombinant vector p-8miR-122 to express miR-122 level and anti-HBV activities were analysed by RT-PCR、ELISA、Sourthern blot. Through adenovirus packaging、amplification、purification and titer detection，the pDC312-cmv and p-8miR-122 adenovirus had a high purity，high miR-122 expression levels and significantly anti-HBV activities.

Abstract:The samples suspected infection of FMDV were identified by RT-PCR method based on VP1 gene. One of the positive samples was sequenced and applied to homologous analysis with other reference sequences, to construct phylogenetic tree of VP1 gene. The results showed that the isolated VP1 belonged to serotype A, Asia topological type, and Sea-97 lineage. It has a high sequence similarity (99.1%) to A/GDMM/CHA/2013, a Chinese A type FMDV isolated in 2013, whereas has a low sequence similarity to the vaccine strain AF/72 (79.6%). The low sequence simi-larity between vaccine strain AF/72 and the A-type FMDV viruses reported recently indicated that AF/72 vaccine strain might only provide limited protection and the vaccine strain should be made up to date as soon as possible.

Abstract:Simian virus 40 (SV40) is a member of the Polyomaviridae family .Express the structural protein VP1 of SV40 in Bac-to-Bac baculovirus expression system. The expressed protein will be self-assembled to SV40 virus-like particles (SV40 VLPs). Then it will be extracted and purified by ultracentrifugation and molecular sieve. VP1 specified band could be observed at 46KDa in polyacrylamide gelelectrophoresis (SDS-PAGE). Indirect immunofluorescence assay (IFA)proved that VP1 VLPs combined with fluorescein isothiocyanate(FITC)-labeled goat anti-mouse antibody to present green fluorescence. This indicated VP1 protein had good antigenicity. The purified VLPs could be observed under electron microscope with 45nm diameter, which indicated SV40 VLPs was successfully assembled. The Western-blot showed SV40 VLPs could react with human-derived anti-SV40 plasma, which also indicated VLPs had good antigenicity.

Abstract:A SVM based approach is proposed to rapidly identify Gram-negative bacterial secretion system proteins. With the optimization feature set consisted of amino acid composition (AAC) and position specific scoring matrix (PSSM), this method adequately takes sequence and evolution information of proteins into account. Experiments show that this method has a good performance on prediction of Gram-negative bacterial secretion system proteins, which served as a useful complement to the study of bacterial secretion system.

Abstract:The yield of polysaccharide from Crocus sativus petals was 3.75% by water extraction and alcohol precipitation. The purity of crude polysaccharide was 68.34%, measured by anthrone-sulfuric acid method and DNS method. The antioxidant activity in vitro of crude polysaccharide was also determined. The results showed that crude polysaccharide exhibited certain antioxidant activity with the ORAC value of 0.572 μmol TE/mg, and had effect on scavenging ABTS+, DPPH and hydroxyl radical with the half elimination concentration (EC50) of 76.26 μg/mL, 165.5 μg/mL and 880.4 μg/mL respectively.

Abstract:Abstract: Owing to the global climate change, frequent drought and salt stress have happened to most plants. As a result, it has become a hot issue in agricultural field to research in tolerance mechanisms of plants and to cultivate new plant materials that can tolerate salt and drought. Derived from rape, BnTR1 is an E3 ligase located in the membrane, and its expression can be induced by heat stress,and then gives the heat resistance to plants. BnTR1 was transformed into Nicotiana benthamiana to study the growth of transgenic Nicotiana benthamiana under drought and salt stress.What’s more, compared with the control, this transgenic plant shows a better resistance to mannitol and PEG, especially in growth status and accumulation of biomass under PEG stress. After 20 days under drought treatment, plants were rewatered and then cultivated for 2 weeks, and all transgenic Nicotiana benthamiana were recovered from wilting, and all wild type Nicotiana benthamiana died. These results indicated that Nicotiana benthamiana can be resistant to drought with BnTR1. When leaves were treated with hydrosaline solution, transgenic Nicotiana benthamiana could maintain green even as salt concentration was above 600mM, while control plants became albino. This result suggested that chloroplast and chlorophyll in transgenic Nicotiana benthamiana can be more stable under salt stress, and transgenic Nicotiana benthamiana can be more resistant to salt stress. After heat shock, the expression of HSP90 and HSP70 in transgenic Nicotiana benthamiana and controls were all up-regulated, and there were no significant difference in increment between transgenic plants and controls. The expression of HSF30 and sHSP17.6 were also induced which can’t be detected in normal condition, HSF30 increasing 50% and sHSP17.6 increasing 30%. These researches illustrated that Nicotiana benthamiana can survive in many stressful conditions when transformed with BnTR1. This experiment laid a good foundation for the study of BnTR1, and also provided an experimental proof for its application.

Abstract:A lactate-utilizing bacteria BEY8 was isolated from the pit mud and produced butyrate with lactate. Based on phylogenetic analysis of 16S rRNA gene sequences, strain BEY8 should belong to the genus Clostridium. The most closely related type strain is Clostridium tyrobutyricum DSM 2637, with 99.8％gene similarity. The optimal values of pH for bacterial growth and lactate metabolism were found to be 5.5-6.0. The lag time of bacterial growth and lactate metabolism were observed at pH 4.8 and pH6.5, but finally lactate could be converting to butyrate as main metabolic product. In addition, when the concentration of lactate was more than 156 mM, the lag time of bacterial growth and lactate metabolism were also observed, and the arrearage time increase as increasing lactate concentration, but finally lactate could mainly be convert to butyrate as metabolic product. It was suggested that the isolate has a potential to adapt environment change, and these environmental factors do not affect the change of bacterial metabolic pathway. Furthermore, at pH4.8, the addition of acetate can significantly reduce the lag time of BEY8 growth and improve the metabolic rate of lactate. But acetate could not serve as the sole energy source for bacterial growth, and just as electron acceptor in metabolism of lactate and butyrate production.

Abstract:In order to screen the sex-related ISSR molecular marker in Idesia polycarpa Maxim. var. vestita Diels，the inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) system was established and optimized．And we found the best ISSR-PCR reaction system with total volume of 25μL was 55 ng DNA template， 1U Taq polymerase，2μL dNTPs tendency（2.5 mmol/L)，2μL primers (10μmol/L)，2.5μL 10 x Taq Buffer (including magnesium ion)and then adding ultrapure water to make up total 25μL．With this system we discovered only one ISSR primer——UBC841，which could amplify one stable female-special fragment，with size of 250-300bp．By sequencing the female-special fragment，we found the fragment was made up of many fragments with similar sizes，which indicated the female-special fragment showed by PAGE  electrophoresis was a mixed bands．Even so，the primer UBC841 still can be used for sex-related markers to identify the gender of Idesia polycarpa Maxim. var. vestita Diels．