• Volume 54,Issue 1,2017 Table of Contents
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    • >Mathematics
    • Conservative Weighted Finite Difference Scheme for the Rosenau-RLW Equation

      2017, 54(1):1-6.

      Abstract (1108) HTML (0) PDF 650.00 K (625) Comment (0) Favorites

      Abstract:In this paper a numerical method for an initial-boundary problem of the Rosenau-RLW equation is considered. A three-layer weighted conservative difference scheme is proposed. The scheme simulates the conservation property of the equations. The existence of discrete solution is discussed. The priori and error estimates of the discrete solution are derived, and the second order convergence and unconditional stability of the discrete solution are analyzed by the discrete energy method. Numerical examples verify the reliability of the scheme, and that the accuracy of the calculation can be improved by adjusting weighted coefficient properly.

    • Optimality of approximate solutions in set-valued optimization involving subarcwise connected cone-convexity

      2017, 54(1):7-11.

      Abstract (940) HTML (0) PDF 407.57 K (386) Comment (0) Favorites

      Abstract:This note deals with the optimality conditions of approximate solutions in set-valued optimization problems involving generalized arcwise connected convexity in terms of the contingent epiderivative. Firstly, the concept of subarcwise connected cone-convex set-valued mapping is introduced. Then, the two roperties of subarcwise connected cone-convex set-valued mapping are derived. Finally, the sufficient optimality conditions are established for weak approximate efficient and strong approximate efficient elements respectively under the assumption of subarcwise connected cone-convexity.

    • Three-level average linear difference scheme for the general improved KdV equation

      2017, 54(1):12-18.

      Abstract (940) HTML (0) PDF 610.92 K (399) Comment (0) Favorites

      Abstract:In this paper, a numerical method for an initial-boundary value problem of the general Improved KdV equation is considered. A three-level average linear-implicit finite difference scheme is proposed and the existence and uniqueness are discussed. It is shown that the finite difference scheme is of second-order convergence and unconditionally stable. Numerical experiments verify the theoretical results.

    • \sigma-regularity of the vertex superalgebra L_{c_m}

      2017, 54(1):19-28.

      Abstract (698) HTML (0) PDF 639.47 K (404) Comment (0) Favorites

      Abstract:Let $L_{c_m}$ be the irreducible vertex superalgebra constructed by the N=2 superconformal algebra with $c_m=\frac{3m}{m+2}$. Drazen Adamovic gave the proof of the regularity of L_{c_m} in 2001.We consider the simple vertex superalgebra L_{c_{m}} and the automorphism \sigma, which satisfy \sigma|_{(L_{c_m})_{\bar0}}=id and \sigma|_{(L_{c_m})_{\bar1}}=-id. We give the proof of the \sigma-regularity of L_{c_{m}}.

    • Existence of solutions to a class of semilinear fractional differential equations

      2017, 54(1):29-34.

      Abstract (975) HTML (0) PDF 243.75 K (413) Comment (0) Favorites

      Abstract:In this paper, we consider the existence of the solutions to a class of two-term fractional initial boundary value problem: (_^c)D_t^α u(t)+ (_^c)D_t^β u(t)=f(t,u(t) ),0β>0.Our discussion is based on the Schauder fixed point theorem and our result improve some recent results.

    • Multiple Positve Solutions of a Discrete Third-order Three-point BVP with Sign-changing Green's Function

      2017, 54(1):35-42.

      Abstract (836) HTML (0) PDF 293.65 K (442) Comment (0) Favorites

      Abstract:In this paper, we consider the existence of positive solutions for a discrete non-liner third-order boundary value problem, which has the sign-changing Green's function. The approach we use is the fixed point theorem in cone.

    • The lower and upper solution method of fractional-order multiple-point differential equation boundary value problem

      2017, 54(1):43-46.

      Abstract (783) HTML (0) PDF 299.80 K (431) Comment (0) Favorites

      Abstract:In this paper,by applying upper and lower solution method,we study the existence of solution of fractional-order multiple-point boundary value problem x^(δ)(t),=f(t,x(t)),t∈[a,b],a>0, x(a)+∑{k=1}^{m}a_{k}x(t_{k})=c where f:[a,b]×R→R is L^(1)-Caratheodory function,δ∈(0,1],c∈R,t_{k}(k=1,2,...,m) are constants and satisfying a0.

    • Traveling wave solutions of a moderate amplitude shallow water wave model

      2017, 54(1):47-54.

      Abstract (1035) HTML (0) PDF 1.91 M (394) Comment (0) Favorites

      Abstract:The author studies the traveling wave solutions of a shallow water wave model with moderate amplitude by qualitative analysis methods of planar dynamical systems. Using the dynamical properties of the integrable systems, we discuss the bifurcation of its traveling wave system, from which we obtain the existence conditions of the bounded traveling solutions, including solitary wave solutions, periodic wave solutions, kink-like wave solutions and antikink-like wave solutions, and the exact expressions of these traveling wave solutions. Furthermore, we simulate these solutions by the Maple 18 software to verify our results.

    • >Computer Science
    • Multi-feature description of adaptive kernels Object tracking

      2017, 54(1):55-60.

      Abstract (1067) HTML (0) PDF 1.63 M (385) Comment (0) Favorites

      Abstract:Analyzed the traditional Mean Shift tracking algorithm in appearance model is sensitive to illumination changes and the disadvantages such as easily accumulated error on appearance model updating, combines the traditional Mean Shift tracking algorithm calculation speed is fast and easy to combination, the advantages of the design of the two different appearance modeling Mean Shift tracking algorithm. The first kind of Mean Shift tracking algorithm using traditional RGB color model to extract the appearance model, the second is not sensitive to illumination change of color and gradient information extraction model appearance. Combining these two tracking algorithm, through the two track of target tracking algorithm weighted target location, and the principle of based on the update of the cooperation of the two kinds of the appearance of the tracker template updates. This not only makes the tracking accuracy has been improved, and the ability to adapt to change the appearance is greatly improved.

    • Construction of Resilient Functions with High Nonlinearity and Optimal Algebraic Degree

      2017, 54(1):61-64.

      Abstract (796) HTML (0) PDF 367.71 K (414) Comment (0) Favorites

      Abstract:Resilient Boolean functions with good nonlinearity and optimal algebraic degree play an important role in the design and analysis of stream cipher and block ciphers. In this paper, based on different lower resilient functions, a new construction method to obtain high nonlinearity resilient Boolean function is given via modifying Maiorana-McFarland (M-M) class bent functions. It is shown that the constructed functions have the strictly almost optimal nonlinearity and the optimal algebraic degree.

    • Parallel processing researches of Medical image based on Spark

      2017, 54(1):65-70.

      Abstract (1019) HTML (0) PDF 1.62 M (473) Comment (0) Favorites

      Abstract:With the growing number of medical images, in order to effectively and quickly process medical images and make all kinds of medical images processing method get quickly transplant and apply. A medical image parallel processing method based on Spark is proposed, which integrate traditional medical image processing method with the Spark. Firstly, preprocess images base on binary conversion method, store images in the HDFS distributed file system. Secondly, implements a transfer function way, which realized image parallel processing quickly, avoid convert the image processing algorithms to the MapReduce algorithms. Thirdly, according to the experiment, medical image parallel processing method based on Spark has preferable adaptability and efficiency, which can adapt to the large-scale image processing.

    • Research on DDoS detection based on BP neural network

      2017, 54(1):71-75.

      Abstract (1355) HTML (0) PDF 759.10 K (566) Comment (0) Favorites

      Abstract:Distributed denial of service attack (DDoS) is a common threat in today’s networks. While such an attack is not difficult to launch, defending a network resource against it is disproportionately difficult. This paper analysis some famous theories and methods on detection of DDoS network attacks systematically based on the fast neural network algorithm. Meanwhile, the attack traffic feature model which is constructed based on the packet length, packet transmission time interval and packet length change rate etc is proposed. Second, a method to optimize the parameters of the neural network error is also proposed by a large number of attempts. Finally, the UCLA dataset is used to carry out the contrast experiment of the parameters before and after the improvement. Experiments show that the proposed method can effectively detect DDoS attacks and has a better generalization ability.

    • Robot Omnidirectional Mobile Obstacle Avoidance Real-time Trajectory Control

      2017, 54(1):76-80.

      Abstract (690) HTML (0) PDF 852.75 K (392) Comment (0) Favorites

      Abstract:This paper presents a Dick Stella algorithm and the Bessel curve of the mobile robot based on real-time obstacle avoidance control algorithm, the algorithm can effectively solve the problem of robot path planning and tracking, to meet the dynamic constraint conditions, has strong robustness. The simulation results show that the improved algorithm makes the robot move time is shortened, the robot obstacle avoidance motion planning improvement.

    • >Electronics and Information Science
    • A method for road extraction from high resolution color remote sensing image

      2017, 54(1):81-88.

      Abstract (671) HTML (0) PDF 3.00 M (857) Comment (0) Favorites

      Abstract:In this paper, a road extracton method is proposed by combining kernel-based Fisher linear discriminant (FLD) classification and shape feature. This method has four main steps: First, the color information of labeled samples is extracted. Then kernel-based Fisher linear discriminant is used to implement feature classification to segment the images into two categories: road and non-road, according to the information extracted before. After that, the road shape optimization features are used to remove erroneous extraction. Finally, morphological processing are used to optimize the extraction results.Experiment results show that the proposed method in this paper can realize the extraction of road from remote sensing image with color information.

    • Analyze Radar New Track Processing Methods in Clutter Environment

      2017, 54(1):89-94.

      Abstract (858) HTML (0) PDF 1.66 M (515) Comment (0) Favorites

      Abstract:Prone to issuing false track problem in clutter environment,this article discusses sector division and new track rules methods when track association, in which perspective of the data processing.Using statistical analysis of multiple cycles to find a balance between accuracy and real time in creating new track. By appropriately extended judgment cycle (sacrifice real-time) to reduce the occurrence of false track (improve accuracy).

    • Modeling and Algorithm Design of the Nonlinear Time-varying Airborne Radar Microburst Target

      2017, 54(1):95-100.

      Abstract (715) HTML (0) PDF 2.92 M (341) Comment (0) Favorites

      Abstract:In order to analyze generating mechanism of microburst wind shear and wind speed estimation and echo spectrum under asymmetric wind field.The mathematical model of wind shear target echo is established by using mesh generation, combined with Doppler effect, the airborne radar wind shear signal processing algorithm is presented. The wind speed estimation and the rain echo power spectrum are simulated under following wind、partial wind、cross wind and against the wind. The simulation results showed that wind-speed alterations is suitable for the actual wind characteristic under following wind、partial wind、cross wind and against the wind.in different range gates. Asymmetric three-dimensional wind shear off the wind rain echo power spectrum distribution with asymmetric wind field in the radial velocity distribution The three-dimensional rain echo power spectrum distribution of wind shear is consistent with the radial velocity distribution under asymmetric field wind.

    • >Physics
    • Controlled driven oscillations of graphite-based NEMS

      2017, 54(1):101-107.

      Abstract (725) HTML (0) PDF 1.36 M (415) Comment (0) Favorites

      Abstract:The conditions controlling the stable oscillation of graphite-based nanoelectromechanical systems (NEMS) are investigated under periodical harmonic driving force with numerical computational methods. The parameters of the system and control force which allow obtaining the sustained stable oscillation at a constant frequency are determined. The operating characteristics of the nano-oscillator are calculated and analyzed for the qual-length and unequal-length graphite sheets. Calculational results show that sustained stable oscillation of the graphite sheets can be realized by properly choosing the amplitude and the initial phase of the periodical harmonic driving force. The methods reported here are believed to have important implications in NEMS design.

    • First principle study on photoelectric properties of CdS with doping rare earth(Sc, Y, La)

      2017, 54(1):108-114.

      Abstract (842) HTML (0) PDF 2.32 M (406) Comment (0) Favorites

      Abstract:By using the first principle pseudo-potential plane-wave method, the photoelectric properties of CdS doped with rare earth (Sc, Y, La) are calculated and analyzed. The calculation results show that: after Sc, Y, La doped, the lattice constants increase, the cell volume also expands. When Sc, Y or La substitutes for Cd, the Fermi energy moves to conduction bands, the band gap widens, the conductivity type is changed into n-type. These indicate that Sc, Y, La is a n-type dopant. The atoms of Sc, Y, La generate a large number of additional carriers near the Fermi energy level in order to improve the electronic structure. Rare earth atoms and the neighboring S atoms form a stronger interreaction and hence a higher covalent banding after incorporation of rare earth. After rare earth doped, the static dielectric constant, absorption coefficient and reflectivity decrease significantly, indicating that the conductivity and transmission increase. These results indicate that rare earth can effectively modulate the photoelectric properties of CdS.

    • Realization of the Unified Quantum Cloning Machine Based on cavity QED

      2017, 54(1):115-118.

      Abstract (703) HTML (0) PDF 556.24 K (344) Comment (0) Favorites

      Abstract:Based on cavity QED, we first propose the unified scheme to implement the 1→2 optimal universal quantum cloning machine for arbitrary input state, the asymmetric 1→2 phase-covariant cloning and the 1→3 optimal asymmetric economical phase-covariant quantum cloning machine for the equatorial input state. During the cloning process, due to atoms interacting with a highly detuned cavity mode, thus the scheme is insensitive to the cavity decay and thermal field.

    • An electron-spin filter based on a Hybrid Magnetic-Electric-Barrier Nanostructure

      2017, 54(1):119-123.

      Abstract (872) HTML (0) PDF 1.14 M (406) Comment (0) Favorites

      Abstract:Based on transfer-matrix method, we investigate the effect of bias on electron-spin polarization in the spin filter, which can be experimentally realized by depositing two ferromagnetic (FM)strips and a Schottky metal (SM) stripe in parallel configuration on top of the heterostructure. We find that both magnitude and sign of the electron-spin polarization are sensitive to the applied bias in this spin filter, which can result in a bias-tunable spin filter.

    • >Chemistry
    • Nonlinear dielectric behavior study of chemical reactions in a strong electric field

      2017, 54(1):131-134.

      Abstract (1150) HTML (0) PDF 565.55 K (518) Comment (0) Favorites

      Abstract:In the presence of a strong electric field, novel phenomena always happen in chemical reactions, including the change of chemical reaction rate and the shifting of chemical equilibria. However, the mechanism is still unknown. This paper studies the interactions between chemical reaction systems and strong steady electric fields. Based on the theory of molecule reorientation, the modified Smoluchowski equation is expanded by the Legendre polynomials. The general expression of polarization is derived by considering the second-order nonlinear response. The nonlinear variation is also discussed through a simple isomerization reaction. The result shows that the ratio between the second-order nonlinear term and the polarization is proportional to the electric field. Meanwhile, the condition for which the result applies is also discussed.

    • The first principles calculation Visible light on photocatalytic properties of Main Group C-Si-codoped TiO2

      2017, 54(1):135-140.

      Abstract (962) HTML (0) PDF 2.00 M (426) Comment (0) Favorites

      Abstract:In this work, the electronic structures, charge density differenceand optical properties of Si and C codoped anatase TiO2 are studied by using the plane-wave ultrasoft pseudopotential method of density functional theory (DFT). The calculated results reveal that C-Si-codoped anatase TiO2 results in band gap narrowing that increases the probability of electronic transition from the impurity energy levels to the conduction band (about 1.7eV), then improves the solar energy utilization, the existence of impurity energy levels in the forbidden gap reduces the carrier transition energy. The C-Si-codoped TiO2 has been helpful for enhancing the absorption coefficient under visible-light region, especially, among the three models, the third model exhibits the largest value of absorption coefficient under visible-light region.

    • >Material Science
    • Synthesis of photocatalytic Er3+:Y3Al5O12/ZnO and Research its Photocatalytic activity

      2017, 54(1):141-146.

      Abstract (863) HTML (0) PDF 2.70 M (364) Comment (0) Favorites

      Abstract:An upconversion luminescence agent Er3+:Y3Al5O12 mixed with the ZnO that utilizing visible light was synthesized by using sol-gel method and the hydrothermal method,and it was charactered by XRD and SEM. Based on the methyl orange, the photocatalytic activity of the Er3+:Y3Al5O12 mixed with the ZnO was researched under the visible light irradiation. In addition, the influence of the calcine temperature、calcine time、the visible light irradiation time and intensity、the dye initial concentration and the amount of catalyst on the degradation rate of the methyl orange were investigated. The results revealed that the degradation rate increased significantly by adding upconversion luminescence agent Er3+:Y3Al5O12 , and the degradation rate was the best when the 10% of the amount of catalyst .

    • Microstructure evolution on tensile of tungsten nanowires with different crystal orientations of [110], [112] and [111]

      2017, 54(1):147-155.

      Abstract (868) HTML (0) PDF 4.49 M (556) Comment (0) Favorites

      Abstract:Mechanical properties and micro-structure fracture mechanism of tungsten nanowires with crystal orientation of [110],[112] and [111] have been investigated with molecular dynamics and embedded atom methods. Simultaneously common neighbor analysis, center symmetry parameters and coordination number has been employed to analysis the evolution of structure. It is concluded that different crystal orientation nanowires have different characters. The elastic modulus, yield strain, yield strength and the fracture stress is decreasing in the sequence of[111], [110]and [112] crystal orientations. It also reveal that all of the stress-strain curves are classified into four stages: elastic stage, damage stage, yielding stage and failure stage. It concluded that crystal orientation has slightly impact on the elastic modulus but has great effect on tensile strength, yield strength and ductility. Interestingly, hardening stage has been found in tungsten nanowire with crystal orientation of [112] at yielding stage. That is to say the strain increase with the stess increase in the hardening stage, the nanowire with crystal orientation of [112] has the trend that recover its capability in strength, but consequently result in the least ductility. In the end the tensile failure mechanisms of the three nanowires have been summarized.

    • Study on the Supercapacitance Performance of Graphene Oxide-pristine SWNT composites

      2017, 54(1):156-160.

      Abstract (851) HTML (0) PDF 1.91 M (454) Comment (0) Favorites

      Abstract:Graphene Oxide-Pristine SWNT composites were fabricated using graphene oxide(GO) as a surfactant to directly disperse pristine SWNT, followed by a freeze-drying method of the sample. The morphology, structure, component and electrochemical property of the compounds were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and electrochemical measurements. The results showed that the electrochemical properties of the compounds were significantly improved due to the insertion of SWNTs into a GO matrix. Through changing the ratio between SWNTs and graphene oxide, the sample corresponding to a SWNT ratio of 10% showed the best supercapacitor property. At such a optimized mixing ratio, the specific capacitance of the nanocomposite was 155 F·g-1 at a current of 0.5A·g-1 in the KOH aqueous electrolyte of 6 mol·L-1 , which was 1.9-fold higher than that of pure graphene oxide (81.5F·g-1). These results clearly demonstrate that GO-SWNT composites prepared by our present simple method is very promising for next generation high-performance energy storage devices.

    • >Biology
    • Cloning and Tissue Expression of gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) in Pigs

      2017, 54(1):161-166.

      Abstract (996) HTML (0) PDF 1.11 M (577) Comment (0) Favorites

      Abstract:The gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) shows the inhibitory effect on gastric acid secretion and incretin action. The aim of this study is to analyze the cDNA sequence of the pig GIP (pGIP) gene, and further examine the tissue expression of pGIP based on RT-PCR method. Result showed that pGIP cDNA is 532 bp in length and encodes a 144-amino acid GIP precursor which contains a signal peptide. RT-PCR assay showed that pGIP mRNA is highly expressed in kidney and gastrointestinal tract. Characterization of the pGIP gene will help to elucidate its physiological role.

    • Inflammatory Cytokine Mediated Expression of Tissue Transglutaminase in Tissue Fibrosis

      2017, 54(1):167-172.

      Abstract (839) HTML (0) PDF 1.09 M (410) Comment (0) Favorites

      Abstract:Tissue transglutaminase (tTG) is an enzyme (EC2.3.2.13) being able to crosslink polypeptides into iso-peptide bonds. Fibrosis is mediated by myofibroblasts, which produce extracellular matrix (ECM) and generate wound contraction. Here we assessed the expression of tGT in human fibrotic tissues, and addressed how tGT is induced in fibrotic tissues. Through immunofluorescent staining we found that exclusive expression of tTG in fibrotic septa of human cirrhotic liver, co-localized with the alpha-smooth muscle (alpha-SMA) positive cells, implying hepatic stellate cells for tTG expression. Absence from normal skin, tTG was highly expressed in the human hypertrophic scar, which was also intimately associated with the alpha-SMA positive cells. In vitro experiment showed that interleukin-1 could induce tTG expression by rat primary hepatic stellate cells (HSCs). Likewise, TNF-α could induce tTG expression by skin epithelial cells. Thus, our work indicates that inflammatory cytokines may play a critical role in induction of tTG in fibrosis formation.

    • The research of gene knockout via homologous recombination in Phaeodactylum tricornutum

      2017, 54(1):173-177.

      Abstract (804) HTML (0) PDF 833.32 K (482) Comment (0) Favorites

      Abstract:Phaeodactylum tricornutum is model organism for marine diatom. It is also one of the most popular energy microalgae. The genome sequence of P. tricornutum had been completed. But, the gene function analysis and gene regulation research of P. tricornutum need further exploration. In this paper, we constructed the glycerol kinase gene knockout vector for P. tricornutum, and successfully converted the vector to wild type P. tricornutum by particle bombardment. After screened, 34 transformations were obtained. The analysis of cell growth showed that extracellular glycerin had no effect on the cell growth of the transformats. Detected by western blot, the results presented that the expression of glycerol kinase were repressed or reduced in transformants. In short, we explored a knockout system for further gene function research in P. tricornutum.

    • Design, synthesis and antifungal activity of novel 2-methyl-3-furancarboxamide derivatives

      2017, 54(1):178-184.

      Abstract (711) HTML (0) PDF 1.02 M (344) Comment (0) Favorites

      Abstract:Abstract: Based on the structure of the fungicide fenfuram, twenty novel 2-methyl-3-furancarboxamide derivatives were designed, synthesized characterized by 1H NMR. Their in vitro antifungal activities were evaluated against five normal phytopathogenic fungi Rhizoctonia solani, Sclerotinia sclerotiorum, Phytophthora infestans, Pythium aphanidermatum, Physalospora piricola by mycelial growth inhibition method. Compounds 1e, 1f, 2a, 2b and 2f exhibited stronger antifungal effect on R. solani with inhibition of 88.6 %, 74.5 %, 78.5 %, 78.7 % and 73.1 %, respectively, superior to commercial fungicides fenfuram (70.5 %). The EC50 values of the five tested compounds against R. solani showed that compound 1e exhibited the most potent inhibition with an EC50 value of 2.824 mg/L which lower than fenfuram (EC50 = 7.691 mg/L). As a result, compound 1e could be the lead compound for the following research.

    • Multilocus genotype (MLG) analysis of Giardia from captive wildlife in Chendu zoo

      2017, 54(1):185-190.

      Abstract (911) HTML (0) PDF 1.18 M (417) Comment (0) Favorites

      Abstract:In order to investigate the infection and genotypes of Giardia from different wildlife in Cheng Du zoo, a total of 146 fresh fecal samples were collected and their genome DNA extracted. The β-giardin, tpi and gdh genes were amplified by nested-PCR and the product were sequenced followed by phylogenetic analysis. As a result, two persian fallows (CDZOO1 and CDZOO4), a deer (CDZOO2), a tortoise (CDZOO3), a raccoon (CDZOO5), a meerkat (CDZOO6) were infected with Giardia. Multilocus genotypes (MLGs) identified assemblages AI-1 in CDZOO1 and CDZOO3; CDZ002 was infected with assemblage A in both β-giardin and tpi loci; CDZOO4 was confirmed as assemblage A at the tpi locus; CDZOO5 and CDZOO6 were identified as assemblage D at the β-giardin locus while assemblage A based on the tpi locus. In conclusion, this is the first report of molecular epidemiology of Giardia in Chengdu zoo. Meanwhile, Giardia from a tortoise and persian fallow are analyzed by an MLG technique targeting three genes (β-giardin, tpi, and gdh). Furthermore, assemblage A maintained in wildlife and transmitted between them have a zoonotic potential.

    • Cell death and respiration inhibition of tobacco suspension cells under NaCl stress may be mediated by extracellular ATP

      2017, 54(1):191-196.

      Abstract (780) HTML (0) PDF 1.19 M (344) Comment (0) Favorites

      Abstract:In the present work, by using BY-2 tobacco suspension cells, the effect of the extracellular ATP on the cell death and respiration inhibition induced by NaCl stress was studied. The results showed that with the increase of the concentrations of NaCl (from 50 to 400 mmol/L),the level of cell death was increased, while the levels of extracellular ATP and respiratory O2 uptake were decreased. The suspension cells subjected to 200 mmol/L NaCl stress were usded to study the effects of the extracellular ATP on the cell death induced by NaCl stress. Compared with the cells under NaCl stress (200 mmol/L), the NaCl-stressed cells with addition of exogenous ATP (20 μmol/L) had lower cell death and higher contents of both extracellular ATP and the respiratory O2 uptake. These results suggest that the cell death and respiration inhibition induced by NaCl stress are related to the change of extracellular ATP, and extracellular ATP can function in mediating the NaCl-induced cell death and respiration inhibition.

    • A Resources Investigation on the Content of Curcin in Jatropha Seed Endosperm Collected from Six Location in Sichuan, Yunnan and Hainan Province

      2017, 54(1):197-202.

      Abstract (803) HTML (0) PDF 1005.73 K (418) Comment (0) Favorites

      Abstract:The ribosome inactivating protein (curcin) expressed in Jatropha curcas L provides advantages, comparing to prokaryotic expression systems, on the study of protein folding and modification for better development and application of the protein. Therefore, the preservation and investigation of the plant resource for future research and application of the protein are necessary. In our study, a resources investigation was implemented, with the seeds of Jatropha curcas L collected from 6 individual locations (Xichang, Panzhihua, Haikou, Sanya, Renli and Dongfang) in Sichuan, Yunnan and Hainan province. The results showed that the average content of curcin in Jatropha curcas seed endosperm from all six locations is 1.8770-2.5602 mg/g, in descending order of Xichang, Sanya, Haikou, Renli, Panzhihua, Dongfang with relatively large differences between each locations. As illustrated in the data analysis, the seeds collected from Renli provided the highest coefficient of variation (14.6189) and the lowest (7.1876) was recorded in Haikou. The traits correlation of the seeds showed that the content of curcin in endosperm was not associated with hundred-seed-weight and kernel percentage and had weak relevance with the oil from whole seed, but a slight negative correlation was found in the analysis of the relevance of the content of curcin and soluble protein in endosperm (α=0.01). Based on these data, we concluded that the seeds in Xichang with a higher curcin expression could be used as a source of material in the research and application in curcin at this stage.

    • Discriminatory Analysis on the Morphology and Major Components of Kernels of the Edible and Oil-type Sunflowers

      2017, 54(1):203-208.

      Abstract (718) HTML (0) PDF 1.04 M (381) Comment (0) Favorites

      Abstract:Edible and oil types sunflower seeds each 20 kinds of was used as materials, a comparative analysis of the difference in seed and kernel size, kernel protein content, fat content, fatty acid composition and relative content, and carbohydrate content. The results showed that: edible sunflower seeds and kernel are large (length of path seeds: 1.75: 0.79cm; seed kernel: 1.18: 0.54cm), testa mostly duplex white pattern; oil seeds and kernel are Small (length diameter seeds: 1.04: 0.52cm; seed kernel: 0.88: 0.45cm), full enrichment seed, the seed coat is black; The average edible content of sunflower seed kernel protein about 19%,, the average oil content of sunflower seed kernel protein about 15%, edible sunflower seed kernel protein content higher than most oil-use; edible sunflower seed kernel oil content ranged from 30.03 percent to 42.37 percent, oil sunflower seed kernel oil content between 30% percent with 47.24% percent; the main component of sunflower seed oil, palmitic acid, stearic acid, oleic acid and linoleic acid; oleic and linoleic acid relative higher levels, a total of about 90%, 70% edible sunflower seeds rich in linoleic acid content of more than 60%, 58% higher oleic sunflower oil content of more than 80%; edible sunflower seed kernel starch content of about 14%, oil sunflower seed kernel starch content of about 13%. Edible and oil types sunflower seeds, although there are some differences on the main ingredient, but there is no strict boundaries, divided mainly on the basis of its seed coat shape, grain size and nibbling food and convenient features performed.

    • Antifugal activity and GC/MS analysis of extracts from Curcuma phaeocaulis stems and leaves against plant pathogenic fungi

      2017, 54(1):209-214.

      Abstract (839) HTML (0) PDF 992.50 K (402) Comment (0) Favorites

      Abstract:To not only compare the in vitro antifungal activities against phytopathogens between ethanol and n-Hexane extracts of the stems and leaves from C. phaeocaulis, but also the differences of their active chemical components. The ethanol extractum of the stems and leaves from C. phaeocaulis had been prepared, then it was not only extracted by n-Hexane, ethylacetate, and methanol, but also the ethanol and n-Hexane extracts from stems and leaves were chemically analyzed by GC/MS. Results indicated that both the ethanol and n-Hexane extract showed well antifungal activity against pathogenic fungi including Fusarium graminearum, Phoma wasabiae, Sclerotinia sclerotiorum and Botrytis cinerea. Relative antifungal activities of three extracts, were compared and the yield of n-Hexane was 64.68%, higher than those of others while its antifungal activity was also the highest. The GC/MS results showed that there were 11 components increased but 23 components decreased in concentrations, and the highest components were curcumenol (24.46%) and hexadecanoic acid, methyl ester (13.84%) in n-Hexane extract. There were 28 components in ethanol extractum and 23 ones in n-Hexane extractum identified by GC/MS analysis.

    • Analysis of comprehensive tolerance to abiotic stresses in Brassica nupus over-expressing BnTR1

      2017, 54(1):215-220.

      Abstract (907) HTML (0) PDF 2.22 M (364) Comment (0) Favorites

      Abstract:BnTR1 is an E3 ligase localized in the plasma membrane, and the previous studies indicated that it played a key role in heat stress response in Brassica nupus, rice and E. coli. In order to investigate the comprehensive resistance characteristic of B.napus over-expressing BnTR1 lines, this research designed a variety of stress conditions to observe and analysis their phenotype. Results showed that compared with the control, the transgenic plant showed a better resistance to hydrogen peroxide, salt, and mannitol. In addition, the activity of superoxide dismutase(SOD),peroxidas (POD) and catalase(CAT) in transgenic plant was higher than the control under salt stress. These results confirmed that the over expression of BnTR1 enhanced the tolerance of Brassica nupus against a variety of stress, which laid a foundation for further research of the function of BnTR1, also provided original materials for breeding new varieties.