Abstract:In this paper we investigate the problem of existence of positive solutions for the nonlinear singular fourth-order three-point eigenvalue problem u^{(4)}(t)=\lambda a(t)f(t,u(t)),t\in [0,1], u(0)=u'(\eta)=u''(1)=u'''(0)=0, where \lambda is a positive parameter and \eta\in[\frac{1}{2},1) is a constant. By using the fixed point theorem of cone expansion-compression type duo to Krasnosel'skii, we establish various results on the existence of single and multiple positive solutions to the eigenvalue problem. Under various assumptions on functions f and a, we give explicitly the intervals for parameter \lambda in which the existence of positive solutions is guaranteed. Especially, we allow the function a(t) of nonlinear term to have suittable singularities.

Abstract:The commonly used linear / constant element cannot be applied in solving elasticity problem because it dissatisfy the LBB condition. In this paper, we derive a stabilization scheme for elasticity problem based on Bochev-Dohrmann-Gunzburger method. We add a consistent term and projection-type stabilization term, which can effectively bypass the inf-sup condition. The advantages of our method are: they do not depend on the space dimension, do not require calculation of higher order derivatives or edge-based data structures.

Abstract:In recent years, the application of mixed integer nonlinear programming problem has been extremely active and very extensive. In this paper, some necessary conditions for a given global minimizer applied to mixed integer nonlinear programming problem with bounded constraints which arises in continuous as well as discrete optimization have been developed. Also, some sufficient global optimality conditions for mixed integer nonlinear programming problem with bounded constraints are established. The global optimality conditions readily apply to problems whose objective functions are generally twice continuously differentiable. If the objective functions are quadratic, then the global optimality conditions become verifiable. Some simple numerical examples can illustrate the significance of the optimality conditions.

Abstract:In this paper, the existence of solutions for symmetric operator equilibrium problems is investigated in topological vector spaces. By virtue of the conditions of natural quasi-C-convex and C-upper semicontinuity , using the KKM Theorem, the existence theorems of solutions for symmetric operator equilibrium problems are obtained. Our results improve and extent the corresponding results that have studied.

Abstract:~In this paper,~ we study the existence of positive solutions for a class of nonlinear second-order Dirichlet problem ~ $$ \left\{\begin{array}{ll} u''-\ a(t)u+f(t,u)= 0,~~\ \ \ 0< t< 1\\[2ex] \ u(0)=\ u(1)=0 \end{array} \right.\eqno $$\~where~$f:[0,1]\times R^{+}\rightarrow R^{+}$~is continuous,~$a:[0,1]\rightarrow R^{+}$~is continuous.~The proof of the main results is based on the fixed-point theorem of cone expansion-compression.

Abstract:Let $x:M\rightarrow R^{n+1}$ be a locally strongly convex hypersurface, given by the graph of a locally strongly convex function $x_{n+1}=f(x_{1},...,x_{n})$ defined in a convex domain $D \subset R^{n}$. Defining the $F$- metric $\tilde{G}=F(\rho)\sum\frac{\partial^{2}f}{\partial x_{i}\partial x_{j}}dx_{i}dx_{j}$ on $M$, we derive the PDEs of the $F$- complete parabolic affine hyperspheres and obtain some Bernstein properties.

Abstract:In this paper, we are concerned with the existence of positive solutions of a coupled system of second-order and fourth-order ordinary differential equations \[ \begin{cases} &~u''''(t)=\lambda f(t,v(t)), \ \ \ \ \ \ t\in (0,1),\&-v''(t)=\lambda g(t,u(t)), \ \ \ \ \ t\in (0,1),\&~u(0)=u(1)=u''(0)=u''(1)=0,\&~v(0)=v(1)=0,\\end{cases} \] where $\lambda$ is a positive parameter, $f,~g\in C([0,1]\times[0,\infty),~\mathbb{R})$. We prove the existence of a large positive solution for $\lambda$ large under suitable assumptions on $f$ and $g$. The proof of our main result is based upon the Schauder's fixed point theorem.

Abstract:Based on the decoupling transformation and the Lie point symmetry group method, the (2+1)-D KD equation is reduced to the (1+1)-D nonlinear PDE. By extended homoclinic test approach, new perturbed non-traveling wave double solitary solutions of the (2+1)-D KD equation are obtained. Also, the dynamic critical point and the non-traveling wave rational function singular solutions in the limitation of parameters are derived. Applying the Hamilton function in 2-D planar dynamical system, we discuss the existence of the periodic solutions for the symmetrical and reduced equation with the wave transformation. Moreover, some periodic solutions are derived by the Tan-function test method, and the perturbed non-traveling wave periodic solutions for the (2+1)-D KD equation are shown.

Abstract:This paper is concerned in constructing a new deterministic model for the stochastic affine variational inequality problems with nonlinear perturbation based on the least absolute deviation method and weighted expected residual minimization method. By means of Quasi-Monte Carlo method, we obtain a discrete approximation of the problem. Some properties of the problem are studied under suitable conditions. The convergence of optimal solutions and stationary points of the approximation problem are also analyzed.

Abstract:Research has found that human’s deceptive behaviors would affect their keystroke patterns. Detecting deceptive behaviors through keystroke patterns is a critical step toward building a cyber information security system in the field of social networking. However, the existing models detecting deceptive behaviors still suffered from the problems of high invasion and low real-time performance. To solve the problems, we first designed an experiment to collect a wide range of stroke features (i.e., single-key features, content features and double-key features) from users’ typing process of short text and then developed a predictive model to detect the deceptive behaviors by using Genetic Algorithms (GAs) and Support Vector Machines (SVMs) as feature selection and model building methods, respectively. The results showed that the developed model could effectively detect the deceptive behaviors with accuracy of 82.86%; all the three categories of keystroke features had contributions to detecting deceptive behaviors. In addition, the effects of cognitive workload and pressure on keystroke pattern of deceivers had also been explored.

Abstract:In order to cut down the localization accuracy problem of wireless sensor network (WSN), a novel node localization method is proposed with back propagation neural network (BPNN). At first, the calculation of node localization is presented by ranging interval and signal strength, and the parameters are rapid solving base on BPNN. Finally, a simulation experiment is conducted to study the influence key factor with NS2 and MATLAB. The results show that, compared other localization algorithm, this method has good suitability, and it could effectively reduce the localization error.

Abstract:In order to promote the network data transmission rate and the energy efficiency of the network, a queue management method is proposed by hybrid Gravitional search algorithm. At first, the indexes of energy efficiency are defined, which are measured the effective information capacity and transmission rate. Then, the queue management method is optimized with hybrid Gravitional search algorithm. Finally, the mathematical simulation is conducted to analyze the influence of queue length and packet size on network energy efficiency. The results show that it has better adaptability.

Abstract:【Abstract】: In hierarchical M2M（Machine-to-Machine）networks with high-density of nodes, the interlayer cluster heads and terminal nodes generally adopt the random mechanism to compete channel, so cluster head or terminal nodes contend for the channel with similar opportunity, especially the cluster head has large amount of data to transmit, which may cause high delay or packet loss. In order to reduce delay and packet loss rate of cluster heads，then presents BT-MAC((Based on Traffic Medium Access Control) algorithm, when terminal nodes and cluster head compete channel, based on the amount of data to be sent algorithm with inverse proportion to set backoff time of the node. So the greater data cluster head transfer, the smaller average backoff time is, equivalent to set cluster head a higher priority, thereby collisions reduce, and the probability of cluster head increase. Simulation results show that BT-MAC algorithm can improve the channel access probability of cluster head thereby to achieve a smaller average delay and can reduce the average energy consumption of nodes, compared to the common DMAC protocol and SMAC protocol.

Abstract:Aiming at the statistical characteristic function and conventional spherical harmonic functions could not effectively describe 3D rock models, rock spherical harmonic functions were proposed. This algorithm was an improvement on the conventional spherical harmonic functions used in 3D model retrieval. Firstly, the 3D rock model was decomposed into a series of concentric spheres. On each sphere, a spherical function was obtained. By restricting the spheres to different radii, a collection of spherical functions could be obtained. Then, spherical harmonic transform was used on these functions to get morphological characteristics descriptor. Finally, a similarity comparison function was used to measure the similarity between each 3D rock models .Experiments on multi-group sandstone core samples show that the proposed algorithm is efficient in describing 3D rock models, and that the depicting ability of the new algorithm is exacter than the statistical characteristic function and the conventional spherical harmonic functions.

Abstract:Tinnitus is a sensation of sound without external stimulation and it is common on clinical .Tinnitus retraining therapy (TRT) is a recognized treatment of relieving tinnitus. Scientific researches show that some similar and unrepeatable music can be used as the sound of TRT .This paper proposed a new method of generating fractal tones based on IFS and broken chord .Applying this new method in the synthesizing could make all the notes within the scale and appeared randomly .Furthermore, the adjustability of neighboring notes avoided the tone mutation. By using this method, the tinnitus rehabilitation sound achieved a balance of predictability and randomness .Experimental results showed that it had obvious fractal characteristics and remained the fractal image features. By adding broken chords, the music turned into more coherent and mildly pleasant songs .So it had reference significance to the treatment of tinnitus.

Abstract:In this paper ,an efficient parameter extraction method of the small signal equivalent circuit of 40nm MOS transistors on the weak-inversion region are presented by using two-port network analysis method in millimeter wave frequency bands. The equivalent circuit is based on a quasi-static approximation, which includes the complete intrinsic quasi-static MOS model, the series gate resistance, source resistance, drain resistance and a substrate coupling network. Device parameters extraction which divided into parasitic parameter extraction and intrinsic part extraction is performed by Y-parameter analysis on simplifying the equivalent circuit for the way of OPEN and SHORT structures. The extracted results are physically meaningful and can be used to de-embed the extrinsic effects such as the substrate coupling .

Abstract:Laser radar has the advantages of high scanning precision,so it is used to obtain the information about the environment around the robot.In order to solve the problems about trough width calculation and pure steering in the VFH algorithm,a method to calculate the trough width and check the traversability of the robot in this trough is proposed.Based on the acquiring goal orientation, local target points are added as the result of the VFH algorithm.In addition,a control strategy of smooth turning is designed,which makes the robot to approach the target position with smooth way in a complex environment.Finally,effectiveness of the method is verified by experiments.

Abstract:The Noether theorems for dynamical systems with non-standard Lagrangians based on El-Nabulsi models are studied. The Hamilton principles with exponential Lagrangians and power law Lagrangians are established respectively, and the Euler-Lagrange equations of the systems are obtained. The formulation of the conditions of Noether (quasi-) symmetrical transformations are given in terms of invariance of Hamilton action under infinitesimal transformation, the Noether theorems of dynamical systems with non-standard Lagrangians are established. At the end of the article, two examples are given to illustrate the application of the results.

Abstract:In order to study the generation of the vector beams by using the polarization modulation of the spatial variant wave-plate, the Jones vectors and Jones matrix are used to further discuss the process, in which an arbitrary Laguerre-Gaussian beam can be transformed into vector beam. Based on the mathematical mode, the far-field intensity distributions of the generated vector beams have been simulated by numerical method. The simulated results show that, the line-polarized and circularly polarized beams with basic mode can be converted to vector beams by using spatial variant half-wavelength plate and quarter wavelength plate respectively, and the dark core of the output beam increases with an increase of the order of the spatial variant wave-plate. The effects of the spatial variant wave-plate on the helical beams can be equivalent to the superposition of two mutually orthogonal circularly polarized helical beams. The polarization test of the orbital angular momentum confirms the practicability of this method.

Abstract:The mechanical properties and the electronic structures of transition metal compounds ZrB3 and NbB3 (m-AlB2, OsB3 and MoB3 structures) under high-pressure are studied by using the first-principles within density functional theory. It was found that m-AlB2 is the most stable structure under normal pressure. The pressure-induced phase transition occurs at the pressure of 40 Gpa, OsB3 structure becomes the most stable one under high pressure. m-AlB2-NbB3 has a maximum shear modulus of 204 GPa, the highest Debye temperature of 921 K, and the highest hardness of 27.3 GPa. The harnesses are estimated to be 24.9 GPa, 22.6 GPa and 19.8 GPa for MoB3-NbB3, OsB3-NbB3 and m-AlB2-ZrB3, respectively. The calculated density of states (DOS) illustrated that the electronic structures of considered compounds are governed by the strong hybridization between M-4d and B-2p states. All compounds are metallic.

Abstract:The current of Schottky barrier metal-oxide-semiconductor field-effect transistor (MOSFET) is popularly calculated through the integration of Fermi-Dirac distribution for carrier over energy or self consistent iterative numerical calculation. In order to reduce the calculation complexity, this paper presents a new analytical drain current model for surrounding-gate Schottky barrier MOSFET through adopting several fitting parameters which takes into account for the impact on the Schottky barrier height of image force barrier lowering effect, dipole barrier lowering effect and quantum effect at smaller size. The proposed drain current model is in good agreement with the reported experimental data in the literature, which verifies the correctness of the model and can provide some reference for the design of surrounding-gate Schottky barrier MOSFET device and circuit.

Abstract:The eigen-wavefunction of the Fibonacci quantum well structure is presented based on an exact solution of the Schrodinger equation by using the transfer matrix approach and the boundary conditions. We consider the case where one of the potential well containing impurities for the Fibonacci quantum wells structure, and we think that the impurities merely changed the well width. For a selected range of parameters of semiconductor materials, the behavior of eigenenergy has been studied for the doped Fibonacci quantum well structure by changing the well width in the one of the potential well containing impurities.

Abstract:Based on the tight-binding model of π electrons which are relevant to sp2 hybridization of carbon atomic orbitals, and restricting k by periodic boundary condition, the model and electronic structure of toroidal carbon nanoscrolls are constructed and analyzed. The results show that the electronic structure of toroidal carbon nanoscrolls is intimately related to its geometric structure, so carbon nanoscrolls can be modified as metallic or semi-conducting materials by controlling its chirality、structural parameters and toroid perimeter.

Abstract:In this paper, we investigate the structure，electronic and optical properties of cubic phase CrN using the plane wave pseudopotential density functional theory method. The results coincide well with the experimental and theoretical values. The band structure, Density of state, dielectric function, refractive index, reflectivity, the absorption, and the electron energy-loss spectrum are obtained successfully and these features are discussed.

Abstract:The defect geometry structure, band structure, density of states and optical properties are calculated in β-FeSi2 system and β-FeSi2 containing Si vacancies by density functional theory of potential plane wave method. The optimized results showed that Si vacancies result in the local lattice distortion, the narrower band gap and a new intermediate band between valence band and conduction band. The slight upward shift of Fermi surface due to Si vacancies leads β-FeSi2 to become a p-type semiconductor. The calculations of optical properties indicate that the electronic structure of adjacent atom is changed, the static dielectric constant ε1(0) increases, the highest peak of ε2 moves to a low-energy region and the tiny red shift of absorption coefficient takes place due to the Si vacancy .

Abstract:By setting different electric fields and adopting density functional B3P86/cc-PV5Z method, the geometric structure of SiH molecule is optimized, and the bond lengths, dipole moments, vibration frequencies and other physical properties parameters are obtained. On the basis of adopting the energy of high precision coupled cluster CCSD (T) method and the same basis set to scan single point energies, the potential energy curves of different external fields are given. The results show that the physical properties parameters and potential energy values will change with the changes of external electric fields, especially, of the reverse direction electric field. In order to get the critical dissociation electric parameter, the dipole approximation is adopted to construct potential model, then the model is put to fit the corresponding potential energy curve of external electric field. It is found that the fitted critical dissociation electric parameter is reasonably consistent with the numerical calculation; i.e., the relative errors are also less than 4%. So the model constructed is reliable and accurate. These will provide some theoretical and experimental reference for the studies of molecular spectroscopy, dynamics and molecular cooling with Stark effect.

Abstract:Carbapenems, used in clinical treatment of multi-drug resistance bacterial infection, can be hydrolyzed by Klebsiella pneumoniae carbapenemases, which weaken the treatment effect of gram negative bacterial infection. It’s an important means of improving the clinical efficiency of these antibiotics to develop novel, potent and specific Klebsiella pneumoniae carbapenemase inhibitors. And β-lactamase inhibitor protein can competitively inhibits Klebsiella pneumoniae carbapenemases. The movement patterns of complex involved in Klebsiella pneumoniae carbapenemase and β-lactamase inhibitor protein are analyzed by coarse-grained models. The results indicate the movement patterns of Klebsiella pneumoniae carbapenemase change a lot after it is inhibited by β-lactamase inhibitor protein. Finally, the binding modes between the series of cyclic boronic acid β-lactamase inhibitors and Klebsiella pneumoniae carbapenemase are released by molecular docking and molecular dynamics simulation, and explained the relationship between the recognition mechanism and conformation-inhibition activity of these inhibitors from the angle of hydrogen bonds and energy. This research provides a theoretical basis of the subsequent design of the inhibitor based on the Klebsiella pneumoniae carbapenemase structure.

Abstract:Abstract:Using Response Surface Methodology(RSM), selecting Quadratic model, on the basis of model Box-Behnken experiment design optimization in NH3-(NH4)2SO4-H2O system leaching of zinc oxide ore experiment process parameters, and the regression equation is established.Regression analysis model of "Prob>F" value of 0.0023, less than 0.05, the model of high precision, all between independent variable and dependent variable of the regression equation is significant, regression equation to be trusted. Model analysis found that the liquid-solid ratio, leaching temperature, leaching time, the three factors that affect the leaching rate of zinc is significant, the analysis of the response surface and contour plot, liquid-solid ratio and the leaching time of interaction is significant.RSM analysis ,the optimum leaching conditions were obtained as follows: liquid-solid ratio 12.81, leaching temperature 30 ℃, leaching time of 4.15 h, total ammonia concentration of 7.5 mol•L-1, predicting leaching rate was 86.2%.Considering the feasibility of the operation, in the NH3-(NH4)2SO4-H2O system leaching process of zinc oxide ore, selected liquid-solid ratio 13, leaching time 4h, leaching temperature 30 ℃, total ammonia concentration of 7.5 mol•L-1, measured the leaching rate was 86.0%, compared with the theoretical prediction error is 0.23%.

Abstract:Many living beings can distinguish direction and position by using geomagnetic field, while biomagnetites can be found in these living beings, so scholars think that there are magnetoreceptions existing in these living beings which are formed by the biomagnetites and outer organic tissues (including the nerve). The magnetoreceptions interact with outer magnetic field and can transform the magnetic information into neural information, so these living beings can have response to it. The theories of biomagnetite magnetoreceptions include the model of single-domain magnetite magnetoreception and the model of superparamagnetic magnetite magnetoreception. In magnetotactic bacteria, there are many typical single-domain magnetites, so in this paper, the magnetic model and working mechanism of the single-domain magnetite magnetoreception are discussed qualitatively from the point of view of theory of magnetism taking the example of magnetotactic bacteria. In the outer magnetic field, each single-domain magnetite which is surround by biomembrane can be forced, and the single-domain magnetite transmits the force to the outer biomembrane and can be accepted by mechanical receptor in the biomembrane, then the force can be transformed into a stimulus signal which can cause the behavior of the bacteria; Another case may also exist, the single-domain magnetites combine with the biomembrane tightly, the force can open or close the ion passageway thereby influencing the inflow of ions and influencing the transmembrane potential, so stimulus signal can be produced; The signals can be magnified because many single-domain magnetites and biomembrane exist.

Abstract:g-C3N4 and P25 organic-inorganic composite with varying the content of g-C3N4 has been synthesized through facile mixing and heating method. The composite was characterized by X-ray diffraction, high-resolution transmission electron microscopy (HRTEM) and UV-vis diffuse reflection spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and BET surface area measurements. The activity of composite photocatalyst g-C3N4-P25 with 60% or 80% g-C3N4 for photoreduction of CO2 is higher than that of either single-phase g-C3N4 or P25 under visible light irradiation. The as-prepared organic-inorganic composite exhibits an improved photocatalytic activity due to enhancement of electron–hole separations both at the interface and in the semiconductors.

Abstract:Li4Ti5O12 was synthesized by the sol-gel process under different conditions, and the as-prepared products were characterized by XRD in order to find out the ideal condition of preparing Li4Ti5O12. The precursor sol was loaded on porous ceramics synthesized from kaolin, talcum powder, alumina and carbon powder, and then Li4Ti5O12 based on porous ceramics was prepared after drying and calcination. Subsequently, porous ceramic-based lithium ion adsorbent was obtained by modification with hydrochloric acid, and then adsorptive capacity of the obtained product was measured. The results indicate that increasing calcination temperature and holding time is beneficial to the formation of Li4Ti5O12. The products with good crystallinity and purified crystal phase are obtained after a calcination at 750 ~ 800 ℃ for 5 ~ 8 h. The extraction rate of Li+ from Li4Ti5O12 and the adsorptive capacity are increased with the increment of calcination temperature and holding time. The Li+ drawn out ratio of the products calcined at 800 ℃ for 5 h is 51.7 %, and the adsorptive capacity reaches 12.2 mg Li+/g Li4Ti5O12.

Abstract:AtTR1, AtCPK28, and AtCPK32 are functioning in the abiotic stress response in Arabidopsis thialiana, and closely associated with the calcium signaling pathway. In order to analyze relationships between AtTR1, AtCPK28 and AtCPK32, we expressed AtTR1, AtCPK28, and AtCPK32 in Escherichia coli, purified expression proteins, and studied the ubiquitination of AtCPK28 and AtCPK32 by AtTR1 in vitro. The results showed that the AtCPK28 and AtCPK32 were multi-ubiquitylated by AtTR1 in vitro. It indicated that the AtCPK28 and AtCPK32 are potential downstream targets of AtTR1 in the regulation of stress resistance signaling pathways. These findings provided experimental evidence to further study the interactions between AtTR1, AtCPK28, and AtCPK32, and also provided some insights of their molecular mechanisms in the plant under abiotic stresses.

Abstract:Protein ubiquitination is one classic type of post-translational modification. The ubiquitin ligase E3 could interact directly with the substrate and mediate in large part the ubiquitination specificity. Arabidopsis thaliana BAH1 encodes a ubiquitin E3 ligase with a C3HC4-RING finger domain, which could bind two zinc ions to form a cross brace zinc finger. Here, we found the zinc finger structure of BAH1 and DnaJ was similar. Further self-ubiquitination assay in vitro demonstrated BAH1 possessed E3 ligase activity. We also found BAH1 can confer heat tolerance of E. coli as DnaJ by temperature-sensitive assays. Hence, these results indicated that BAH1 may improve heat tolerance of E. coli through interacting with DnaK/DnaJ chaperone system.

Abstract:Abstract：Objective The blood products packaging materials of rubber closures in the process of sample store, there might be 2,6-di-tert-butylphenol dissolution, so it is need to establish the 2,6-di-tert-butylphenol testing method of blood products. Method According to the properties of intravenous human immunoglobulin (pH4) and human serum albumin, perchloric acid was selected as precipitant reagent and tributyl phosphate as internal standard to detect the 2,6-di-tert-butylphenol content .Then the method is verified by the specificity, accuracy, repeatability, linearity, limit of quantification, range and intermediate precision. Result The results demonstrated this method was feasible without protein interference, 2,6-di-tert-butylphenol and the internal standard of tributyl phosphate were well separated in this methold. Moreover, each verification result was consistent with the expectation, and the limit of quantitation was 0.3 ng/mL. Therefore, the testing method was proved to be very effective. Conclusion In this work, a simple and sensitive method was first developed and successfully applied to detect the content of 2,6-di-tert-butylphenol from blood products.

Abstract:Abstract: The phytohormone abscisic acid is a key endogenous messenger in plants’ response to abiotic stress by regulating some crucial gene expression. Founded in previous research in this lab, a RING structure of E3 ligase At5g66070, through bioinformatics analysis found could be induced by ABA.So we analyse its related physiological function under the condition of ABA treatment .Gene At5g66070 was studied before in this laboratory. The results showed that the gene expression was significantly increased after 10μM ABA treatment. The cellular localization of At5g66070 was predominantly found in the nuclear,indicating At5g66070 is a nuclear protein. No full-length At5g66070 transcript was detected in the mutant by DNA and RT-PCR analysis, suggesting that At5g66070 is a loss-of-function mutant.Germinated evenly-grown seedlings were then transferred to MS plates supplemented with or without ABA.At5g66070 mutant showed shorter primary roots compared with the overexpression lines under ABA treatment.It means the mutant is more sensitive than wide type and overpression lines. The 10μM ABA treatment induced stomata closure in mutant, but had no effect on wild type and overexpression lines. Using RT-PCR to analyze the related genes in Arabidopsis ABA singnaling pathway.Our results indicate that At5g66070 is a negative regulator of ABA signaling.

Abstract:The dry roots of Angelica decursiva and Peucedanum praeruptorum are used as Chinese traditional medicine for a long time. This study applied MaxEnt (Maximun Entropy) ecological niche modeling to predict the potential range of Qianhu and Zihuaqianhu in China on the basis of occurrence points. The results indicated that: environmental factors such as Mean temperature of the coldest quarter, Monthly mean diurnal temperature range, Min temperature of the coldest month, Precipitation of the driest month, and Precipitation of the driest quarter are greatly influenced the size of Qianhu's and Zihuaqianhu's potential range. Potential distribution areas of two plants are more widely than the realistic distribution areas. Some mountain areas of Qinling Mountains, Nanling and Zhejiang province are the most suitable area (adaptive index >0.71). The difference of distribution in Taiwan, Gansu, Xizang province can provide the basis for the discrimination. With the wild resource reducing and demands increasing, cultivation of plant in the most suitable area is feasible way to increase output of production and protection of wild resources diversity of Chinese medicinal materials.

Abstract:Abstract: In order to study the drought resistance and water saving morphology effects of pacolbutrazol on A. fruiticosa, the pot experiment was carried out of six levels paclobutrazol applied on A. Fruiticosa, the morphology indexes were measured.The results showed that paclobutrazol inhibited effectively the height,leaf area,coverage,crown width,base diameter, increased the numbers.The proper concentration paclobutrazol could increase the coverage, crown width,base diameter,lift water and soil conservation effect and lodging resistance.Total consideration,D4 not only inhibited the height and leaf area of A. fruiticosa, decreased nutrient and water needs and water evaporation,increased drought resistance,increased coverage,crown width,base diameter,leaf colour to improve water and soil conservation and landscape effect, so D4 was the optimum concentration.

Abstract:With the increasing recognization of sncRNA, especially miRNA and piRNA, it has been proved that small noncoding RNA (sncRNA，<200 nt) plays a significant role at many levels including gene expression regulation, alternative splicing, cell growth, differentiation, proliferation, apoptosis and disease, etc. The sncRNA cDNA library construction is the main way to acquire and research sncRNA. Now, there are many sncRNA cDNA library kits on the market but it’s too expensive. Therefore, an improved method of sncRNA cDNA library construction was established: (1)5S and 5.8S rRNA were removed efficiently by RNA Antisense Purification (RAP); (2) More 5′-monophosphorylated RNAs were acquired after using Tobacco Acid Pyrophosphatase (TAP) and RNA 5' sncRNA Polyphosphatase, which resulted in the increasement of the coverage of the cDNA library. (3) To avoid using the adenylated adapter, a poly(A) structure was added to sncRNA 3′ end and then reverse transcription was carried out with oligo(dT)16VN-adapter primers. Finally, the complete sncRNA cDNA sequences were obtained by employing this improved and cost-effective method, which laid the foundation for the further study of sncRNA.

Abstract:Protein biological function is determined by its three-dimensional structure. Any residue substitution may lead to dramatic changes in structure and result in functional alternations. Thus it is of great importance to study the relationship between substitution and structure. Usually, proteins three-dimensional structure prediction is based on the similarity of sequence and structure. Whether this method is valid to the protein with residue substitution is still an open question. To answer this question, single residue substitution crystal structures were extracted from Protein Data Bank (PDB) to construct a non-redundant test set, which was used to assess two homology modeling structure prediction tools, SWISS-MODEL and MODELLER. The results indicate that the two methods perform well in overall structure prediction (RMSD<0.5Å), however, they fail to predict the mutants that own significant structural changes (A_RMSD>1.5Å) upon residue substitutions. The two methods perform better in mutations of embedded residues and mutations between polar residues. Although it is a small fraction of residue substitutions (<5%) that lead to significant conformation changes, they may play important roles in loss or gain protein functions. Therefore traditional homologous modeling methods are not appropriate for predicting such protein structures. There is urgent need to developing more powerful prediction methods.

Abstract:Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L. we can get I, II, III sections. The activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase in insects were determined in vivo. The results showed that I section from Stellera chamaejasme L. had the strongest poison effect on Locusta migratoria manilensis (Meyen). Under the concentration of 10 mg/mL, the corrected mortality was 92.5% in 72 hour. And the poison effect there was persistence. The I section from Stellera chamaejasme L. also had certain inhibition on activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase. The inhibiton rate of Na+-K+-ATPase was the highest among the three kinds of enzymes. There was a well correlation between the toxic action of the active ingredients from Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen) and the inhibition on activity of Na+-K+-ATPase in Locusta migratoria manilensis (Meyen). Na+-K+-ATPase is likely the target of the active ingredients from Stellera chamaejasme L. against Locusta migratoria manilensis (Meyen).

Abstract:The ORF2 gene sequence of NoVs VA387, at a length of 1 623 bp, was synthesized and inserted into vector pFastBac1. Transformed DH10Bac competent cells by pFastBac1-NoV-ORF which was identified by sequencing to obtain Bacmid-NoV-ORF2. The constructed recombinant plasmid Bacmid-NoV-ORF2 was transfected to sf9 cells by co-precipitation with calcium phosphate. Harvested the supernatant of cells and frozen storage recombinant baculovirus at -20 ℃. Tn5 cells were infected by Ac-NoV-ORF2 virus and the cells was harvested 5 d ~6 d after infection. Break the cells, and the supernatant was harvested and purified by MS purification. Various components were collected, observed by electron microscopy，and determined for protein content by 10% SDS-PAGE. The Tn5 cells transfected with Ac-NoV-ORF2 virus showed specific protein by 10%SDS-PAGE profile. The capsid protein expression was significantly and gradually increase since 3 d after infected, and the production reached stable in 4 d. The 10% SDS-PAGE showed that the capsid protein was mainly located in two bands with relative molecular masses of 59 KD and 56 KD. Electron microscopy showed that the expressed capid protein was assembled to VLPs at sizes of about 40-50 nm. So the capsid protein of NoVs was successfully expressed in Tn5 cells and assembled into VLPs.