Abstract:In this paper the global qualitative properties of a system which models a genetic toggle switch with cooperativties $1$ are given. Firstly it is proved that the system has a unique equilibrium, which is a stable node. Then it is shown that there are no periodic orbits by the Poincare-Bendixson Theorem, and the system has exactly two equilibria at infinity, which are both saddle-nodes. Consequently, the global phase portrait indicates the system is globally monostable.

Abstract:It is very important and interesting to construct the exact solutions of the (3+1)-dimensional space-time fractional mKdV-ZK equation.Firstly, by using fractional complex transformation, mKdV-ZK equation was transformed into a nonlinear ordinary deferential equation, and then introducing extends (G'/G)-expansion method to construct the exact solution. A series of new exact solution for mKdV-ZK equations have been obtained.

Abstract:In this paper,~we study the existence of positive solutions for second-order three-point boundary value problem \[ \begin{cases} u''-k^{2}u+a(t)f(u)=0,~~t\in(0,1),\u(0)=0,~~u(1)=\alpha u(\eta), \end{cases} \] where~$a\in C([0,1],[0,\infty)),~\eta\in(0,1),~\alpha\in\Big(0,\frac{\sinh(k)}{\sinh(k\eta)}\Big),~f\in C([0,\infty),[0,\infty))$.~The proof of the main result is based on fixed point theorem.

Abstract:In this paper, a conservative difference scheme for an initial-boundary value problem of the general improved KdV equation is proposed. This scheme simulates the two conservation properties of the problem well. Existence and uniqueness of numerical solutions are derived. By method of discrete energy, second order convergence and unconditional stability are discussed.Numerical examples show the availability of scheme.

Abstract:In this paper, we study the following nonlinear equation with mean curvature-like operator$$\frac{\partial q(x,t)}{\partial t}+\frac{\partial}{\partial x}(\frac{\frac{\partial q(x,t)}{\partial x}}{\sqrt{1+(\frac{\partial q(x,t)}{\partial x})^{2}}})-g( q(x,t))=0.$$ By using the theorem of the monotone dynamical system, the existence conditions of traveling wavefronts is established.

Abstract:In this paper we consider the dynamics of a class of one-dimensional discontinuous nonlinear maps. We obtain the existence and stability conditions of fixed points in the parameter space. Then we study the existence and stability of period-m orbit of type $A^{m-1}B$. Analytical conditions for the existence and stability of such kind of periodic orbits are found. The period doubling and saddle-node bifurcations of those orbits are also studied.

Abstract:In this paper, the numerical solution of initial-boundary value problem for generalized Rosenau-KdV-RLW equation with non-homogeneous boundary is considered. A nonlinear two-level Grank-Nicolson difference scheme is designed. The difference schemes simulate two conservative quantities of the problem well. The existence and uniqueness of the difference solutions are also proved. It is proved by the discrete energy method that the difference scheme is second-order convergence and unconditionally stable. Numerical experiments verify the theoretical results.

Abstract:In this paper, we apply combined hybrid finite element methods to the eigenvalue problems, and construct a new finite element method for solving the smallest eigenvalue. First, we derive the optimal error estimation, and then we use numerical examples to verify our theoretical results. Both theoretical analyses and numerical results show that for all the combined coefficient , our methods can obtain second order accuracy for solving the smallest eigenvalue when the lowest order finite element spaces are used. From the numerical performance, we can also observe that the numerical solution can approach to the exact eigenvalue from both directions for different , so we can choose the optimal such that better approximations can be obtained on the coarse grid.

Abstract:For the issue of less effective usability comprehensive evaluation on users' comments with uncertain information and complex evaluation indexes, a users' satisfaction comprehensive evaluation model is applied on usability research of production, which integrates gray relational analysis and AHP (Analytic Hierarchy Process). In order to enhance the objective of gray relational analysis, AHP method is used to determine the relative weights of evaluation index so as to establish comprehensive evaluation model of users' satisfaction model. With contrast experiment on the dataset of smart mobiles in different price, the satisfaction rank from our model is very similar to the one of actual sales. In addition, experiment result shows gray relational analysis can distinguish the satisfaction difference of productions more clearly. So above experiment results illuminate our model can comprehensively effectively evaluate users' satisfaction of production with incomplete assessment information

Abstract:In order to improve the samples diversity and convergence properties of social spiders optimization algorithm (SSO), an adaptation social spider optimization algorithm based on dynamic multi-swarm strategy (DMASSO) is proposed. According to the algorithm samples diversity and evolutionary level, the spider population is dynamically divided into different sizes leading groups and supporting groups, and the adaptive learning factor and Gaussian disturbance factor are introduced to improve the algorithm update ways, which helps to improve the algorithm global optimization ability and maintain the diversity of the sample population. For the test results of typical characteristics functions show that compared to SSO algorithm, SFLA algorithm and other optimization algorithms, the new algorithm has better convergence speed and convergence accuracy.

Abstract:Spectrum-based fault localization (SBFL) techniques aim at identifying the executing programs codes that correlate with failure. A dichotomy matrix for SBFL records the bivariate frequency distribution of the test case results and the program element hit numbers. Given the matrix, many SBFL association measures are proposed to compute suspiciousness scores of the program elements. Research shows that any association measure can't be statistically better than other measures when localizing buggy program. Therefore, a technique based the classification algorithm is proposed which can automatically learn the specific measure for a program set. A sample in training dataset is constructed by employing a pair of faulty statement and non-faulty statement ones, and its features are the probability features difference of two statements. It is evaluated with three benchmark datasets: Siemens suite, space and gzip. Experimental result indicates that the learned measures with LibLinear, Logistic, SGD and SMO of Weka outperformed existing SBFL association measures.

Abstract:In order to improve the intrusion detection rate of industrial control system, the principle of traditional industrial intrusion detection technology is discussed, and the comparative study is done from the viewpoint of information theory. The dynamic and static fingerprints of industrial control attacks in the protocol stack, statistical characteristics, and communication behavior are summarized based on the modeling of the specificity of the industrial control system and the attack methods. Based on a new abstract method of heterogeneous information, a heuristic industrial control system anomaly detection model based on combinatorial neural network is implemented. The test results show that the proposed model is more efficient, and the results are more accurate than the conventional intelligent methods.

Abstract:A ferrite reflector-based optimization design of magnetic resonant wireless power transfer (WPT) system to improve transmission efficiency is proposed. The WPT system composed of four resonators is considered as a two-port network to deduce the transfer efficiency. Then a magnetic resonant WPT system whose receiving and transmitting coils have a diameter of 30cm is designed and manufactured. The measured transmission efficiency is above 90% at the distance of 30cm while decreases to about 40% when the distance increases to 60cm. Measured results are in good agreements with simulated ones. Based on this WPT system, two different ferrite reflectors are introduced to improve the transmission efficiency. Results show that the different size of magnetic reflectors may have positive or negative effects on transmission efficiency. By optimizing the ferrite reflector, the maximum 15% improvement of transmission efficiency is obtained at the distance range of 30cm to 90cm.

Abstract:The cancellable fingerprint bio-cryptosystems based on the fingerprint binary string is study under the premise of protecting the security of fingerprint privacy. During the registration, first detect the fingerprint singular point as the base point, and extract the binary string from the fingerprint as the fingerprint feature, then generate the fingerprint key and auxiliary information by combining the error-correct code and fuzzy scheme. Finally, during the authentication, the fingerprint key could be recovered by auxiliary information and homology fingerprint. Experiments demonstrate that the proposed biometric cryptosystem has good recognition performance. And security analysis indicates that the security strength of the proposed biometric cryptosystem can satisfy the need of current security circumstances.

Abstract:The SOI strained silicon schottky source/drain MOSFET with high-k gate dielectric is a potential device realizing small size MOSFET, which combines the advantages of strained silicon engineering, high-k gate dielectric, SOI structure and schottky source/drain. A model for the structure is proposed by solving two dimensional Possion’s equation, which takes into account for the impact on the source/drain schottky barrier height for electron of image force barrier and size quantization effect, then the drain induced barrier lower model is investigated based on the threshold voltage model. The experiment data of drain induced barrier lower extracted from literature is compared with the model, which verifies its correctness, and discuss the variety relationship between drain induced barrier lower and several parameters. The result shows that drain induced barrier lower increases with strained silicon thickness, channel doping and germanium fraction increasing, decreases with channel length, gate dielectric constant, intrinsic schottky barrier height for electron and drain source voltage increasing. The device can suppress drain induced barrier lower effect greatly through adjusting the model parameters properly, which can provide some reference for the design of SOI strained silicon schottky source/drain MOSFET with high-k gate dielectric device and circuit.

Abstract:The fast and accurate identification of three-dimensional objects is a hot research topic. According to the characteristics of local feature transform, one new method called the invariant angle contour line was proposed. Local segmentation of the objects was realized by point cloud vector feature, and then using Euclidean distance, measuring the distance and angle of the three variables, the invariant point outline feature description was established, after further extracting invariant moments feature, the feature vector database would be constructed. Through the cosine matching between feature description of the object and database, the object recognition can be accomplished by comparing the threshold value. The recognition experiment results show that the algorithm has a high recognition rate and recognition efficiency, and can be used for complex point cloud objects recognition.

Abstract:Using two different methods, Frenkel-Kontorova (FK) model and equivalent coupled-oscillator model based on two-mass-two-spring model, we solve the dynamics of energy dissipation for several types of coupling between the tip and the substrate. We find the unstable energy dissipation phenomenon on the nanoscale and present a microscopic analysis of properties of energy dissipation via average power dissipated and displacement of the particles relative to the center of mass for sinusoidal potential as well as multiple-well periodic substrate potentials. The results are compared with the observations in recent experiments based on friction force microscope (FFM).

Abstract:We employ the first-principles plane wave method in the frame of density functional theory (DFT) to investigate the equilibrium lattice structure, the mechanic, thermodynamic and electronic properties of CuAlSe2 compound. The lattice constants, the bulk modulus B0 and its pressure derivative B0’ are calculated. Based on the quasi-harmonic Debye model, we also obtain thermal expansion coefficient. It is noteworthy that the variation tendencies of these parameters are investigated detailed with pressure. In addition, we also calculate the elastic constants of CuAlSe2 compound. Results show that the elastic constants C11、C33、C12 and C13 increase with increasing pressure, whereas the influences of pressure on elastic constant C44 and C66 are not obvious. The calculated elastic constants satisfy the hecriterion of mechanical until the pressure grows to more than 15GPa, which suggests the structure phase transition of CuAlSe2 may be happened. We also investigate the shear sound velocity VS, longitudinal sound velocity VL, and Debye temperature ΘE from our elastic constants, as well as the thermodynamic properties from quasi-harmonic Debye model. We obtain the dependence between the heat capacity Cv and pressure, and the same as thermal expansion coefficient α. At last, the pressure dependences of band structures and density of states are also investigated, which shows that the value of TDOS decreases with the increase of the pressure.

Abstract:The structural properties of XeF2 in the pressure range from 0 to 80 GPa are studied by plane-wave pseudopotential density functional theory method. The calculated values are in agreement with the experimental data. Based on the calculated elastic constants of XeF2 under different pressures, the I4/mmm structure of XeF2 is confirmed to be stable in the pressure range from 0 to 80 GPa. The band gap of XeF2 at different pressure is calculated and the band gap is found to decrease with the increase of pressure. When the pressure is greater than 10 GPa the band gap of XeF2 increases linearly with the increase of pressure, which indicates that XeF2 transforms from the insulator to semiconductor and has more and more strong metallic with the increase of pressure.

Abstract:We realize the quantum information splitting with respect to phase parameter through the quantum channel with multi-partite entangled states in the condition of the environmental noise. The protocol of quanutm information splitting is that the information encoded in quantum parameters from a sender can be recovered at any one of many receivers by means of measurements. It is found that a general partial measurement can help to enhance the quantum Fisher information under the influence of amplitude-damping noise. In the Markovian case, the values of quantum Fisher information are decreased monotically.In the non-Markovian case, there exists the oscillation of quantum Fisher information.

Abstract:The structural phase transition, elastic and thermodynamic properties of NaH were investigated by the plane-wave pseudopotential method within the density functional theory. The equilibrium lattice parameters a0 , elastic constants Cij, bulk modulus B0 and it’s derivative B0′ were calculated. The results are consistent with the experimental and theoretical data. From the usual condition of equal enthalpy, the phase transition of NaH from B1 to B2 phase occurs at 30.5 GPa with a volume collapse of about 4.5%, consistent with the experimental (29.3±0.9GPa) and calculated (37.0 GPa) results. The static finite strain technique was used to calculate elastic constants Cij at the different pressures. Furthermore, the elastic wave velocities, Debye temperature Θ and elastic anisotropic factors of NaH were also investigated in detail. Through the quasi-harmonic Debye model, in which the phononic effects were considered, the thermodynamic properties such as the specific heat capacity and thermal expansion coefficient of NaH were obtained in the whole pressure range from 0 to 50 GPa and temperature range from 0 to 1500 K.

Abstract:By using the home-made experimental setup with aqueous jets and single pulse laser-induced breakdown spectroscopy(LIBS) technique, we detect the Al trace element in AlCl3 aqueous and systematically study the impact of experimental parameters of laser energy，ICCD probe delay and gate width. The optimum experiment conditions for application of LIBS to the trace analysis in AlCl3 aqueous are obtained.

Abstract:The relative permittivity of dimethyl sulfoxide (DMSO) solutions, ethanol (C2H5OH) solutions and their mixtures have been measured at 20 ℃, respectively. Surprisingly, at certain concentration, it appears an exceptional phenomenon that the imaginary part of mixtures relative permittivity is larger than that of their pure components. To explain the exceptional phenomenon on the mixtures relative permittivity, a systematical investigation about the molecular clusters ((DMSO)m(C2H5OH)n) as well as their cluster radii have been given using DFT calculations. Comparing the cluster radii of the binary clusters ((DMSO)m(C5H5N)n) with that of the pure clusters ((DMSO)m and (C2H5OH)n), the results have given an reasonable explanation of the exceptional phenomenon on the imaginary part of the mixtures relative permittivity.

Abstract:The adsorption of rare earth metal cerium ions by low fat pectin was determined. The effects of the adsorption time, pH of the cerium ion solution, adsorption temperature, dosage of pectin and concentration of cerium ions on the adsorption capacity of pectin were investigated. The adsorption process was analyzed by the Langmuir and Freundlich models. Using the adsorption isotherm, the related thermodynamic and kinetic parameters were calculated, and infrared spectrum analysis was performed. The results indicate that the variables studied significantly affect the adsorption of cerium ions by pectin and that the infrared spectra before and after adsorption are apparently different, implying that a chemical reaction may have occurred in the adsorption process. The Freundlich equation is better suited to describe the adsorption process compared to the Langmuir equation. The adsorption process is best described by a pseudo-first order kinetics equation. Enthalpy and entropy differences are the driving forces of the adsorption process.

Abstract:In this paper, experimental study on static absorption was carried out for Alashan clay rock on Inner Mongolia. To explore the factors which may influence absorption characteristic of U(Ⅵ) on clay rock, including contact time, U(Ⅵ) initial concentration, solid-liquid ratio, pH value, ionic type and ionic concentration. The following results are obtained. The absorption rate of U(Ⅵ) on Alashan clay rock in Inner Mongolia is quite fast with adsorption equilibrium achieved in 24 hours. The optimum adsorption ratio is 1:200, and the optimum adsorption concentration is 160 μg•mL-1.The absorption capacity of clay rock was enlarged with the increasing of pH value. When pH value is at 8, the ability of absorption reaches the best. The absorption capacity of Uranium is largely hindered by Ca2+、CO32- and HCO3-, which have a great effect on the absorption ability of U(Ⅵ). The distribution coefficients of K+、Na+、Cl-、NO3-、SO42- can be changed with the change of ionic concentration, The surface adsorption of U(Ⅵ) on clay rock is dominated by inner-sphere complexation. The studies indicate the adsorption behavior of U(Ⅵ) on clay rock has a great significance on settling high level radioactive waste in China.

Abstract:Chlornitrofen is a diphenyl ether herbicide, which is a typical persistent organic pollutant and has been widely used in 70~90 years in twentieth Century. Although it has been banned for 20 years, chlornitrofen still exists in water and soil systems, which has the potential threats to the agro-ecology systems. In the present study, humic acid ester ether (HAEE) was synthesized via regulating the dosage of glycerol triglycidyl ether while humic acid ester (HAE) was uniting as raw material. HAEE was analyzed by the analysis methods of Fourier transform infrared spectroscopy and contact angles. The results indicated that hydrophobic HAEE has the structure of humic acid and the characteristic functional groups of HAE. The adsorption characteristics and mechanisms of HAEE to chlornitrofen were investigated via a series of batch equilibrium experiments. As the results showed, the hydrophobic angle is increased from 30 to 96 degrees before modification. The degree of hydrophobic of HAEE is positively correlated with the adsorption capacity of chlornitrofen, and equilibrium reached after 2.0 h. The kinetics of adsorption is described well by the pseudo-second-order model, and Freundlich models are more suitable for the sorption isotherm. The adsorption capacity reduces gradually after 5 cycles. The removal rate is still above 78%. The adsorption mechanisms are π-π interaction and hydrophobic distributional effects. Adsorbent prepared by this method may provide a novel approach for remediation of chlornitrofen in aqueous-soil environment.

Abstract:WS2/TiO2 composite was prepared by using the ultrasonic dispersion method, and the effect of degradation of Rhodamine B was studied under irradiation conditions. The influence factors on the degradation efficiency, such as calcination temperature and time of WS2/TiO2 , the amount of catalyst, illumination time, irradiation intensity, and initial concentration of Rhodamine B were discussed, respectively. The experimental results revealed that the best degradation efficiency of Rhodamine B based on the amount of catalyst was 1.00 g/L, the initial concentration was 10.00 mg/L, the m(WS2):m(TiO2) was 1:11, and the calcination temperature and time were 350 °C and 90 min, respectively.

Abstract:Tantalum boride has been sintered under high temperature and high pressure (HTHP) conditions by using a DS6×8 MN cubic press device, and the Vickers hardness of TaB has been measured by using a diamond indentation method. Under the applied load of 29.4 N, our test gave the Vickers hardness of TaB are 7.7±0.4 and 10.5±0.7 GPa, at 4 GPa and the temperatures of 900 and 1100 ℃, respectively. Our result shows that the density becomes better with the increasing temperature under the same pressure. By using the Archimedes method, we calculated the relative density of 13.5 g/cm3 for TaB.

Abstract:ABscisic acid responsive RING-v protein 1(ABRv1) in Arabidopsis thaliana encodes a E3 ligase with a RING finger structure domain. In an effort to analyze the function of ABRv1 in the plant, we performed abscisic acid (ABA) stress experiments on the wild-type, the ARBv1-overexpressing lines and the T-DNA insertion mutants abrv1. The overexpressing lines were acquired through transfection by Agrobacterium with ABRv1 embedded plasmids (pBI121- ABRv1). The mutants obtained were identified by DNA and RNA analysis. With ABA treatment, the mutants presented a lower germination rate than the wild-type while the overexpressing lines showed a higher rate. Little stomatal closure was observed in the the mutants whereas obvious closure was discovered in the overexpresssing lines. The wild-type showed a closure status in between. Therefore, our phenotypic analysis revealed that ABRv1 might participate in the plant response to ABA as a negative regulator.

Abstract:Carboxylic amides are one of the most important classes of agricultural fungicides. To discover new carboxylic amide analogues with high activity against plant pathogenic fungi, twenty novel fenfuram analogues containing a diarylamine moiety were designed, synthesized and characterized by 1H NMR. Their antifungal activities in vitro were evaluated against four phytopathogenic fungi（Rhizoctonia solani, Sclerotinia sclerotiorum，Pythium aphanidermatum and Fusarium oxysporum）by mycelium growth inhibition method. Most compounds showed significant antifungal effect on R. solani and S. sclerotiorum, however, slightly weak antifungal effect on B. cinerea and F. oxysporum. Compound 1i exhibited the most potent antifungal effect on S. sclerotiorum with EC50 value of 1.9229 mg/L, superior to commercial fungicides boscalid (EC50 = 2.6736 mg/L) and lead fungicide fenfuram (EC50 = 9.9667 mg/L). The present work demonstrated that fenfuram analogues containing a diarylamine moiety could be used as a lead structure for further developing novel fungicides.

Abstract:About 230bp of DHAR partial DNA segments was obtained from Subtractive hybridization library, which is highly similar with the nucleotide sequence of DNA encoding DHAR gene, the Subtractive hybridization library was constructed by dwarf mutant and wild-type parent. A full-length cDNA sequence of the gene named BnDHAR was cloned by homologous cloning from Brassica napus. BnDHAR was fully consistent with nucleotide sequence of the gene in the published Brassica napus genome. There were no difference in the DHAR full-length cDNA sequence between dwarf mutant and its wild-type parent. We used qRT-PCR technology analysised the BnDHAR gene expression in different organizations at various periods of Brassica napus , the results showed that: BnDHAR gene expression in the wild-type Brassica napus leaves is the highest approximately 5 times in the dwarf mutant at the seeding stage, and extremely low expression in roots and stems, dwarf mutant and tall wild-type didn’t have any significant difference. Under the high temperature stress, the expression of the BnDHAR gene increased; under the salt stress, BnDHAR reached the highest expression after dealing with 9h, then showed a downward trend; under the drought stress, the expression reached the highest after 12 hours, also showed a downward trend after the first rise. In summary, the BnDHAR gene exhibited a tissue specific expression in Brassica napus and also related with growth and abiotic stress response.

Abstract:The volatile flavor of kiwifruit is a very important taste quality. The changes of flavor components of Hongyang kiwifruit at different brix levels, and the relationship between the expression of Lipoxygenase (LOX， EC1.13.11.12）and flavor changes were investigated. With the measurement of a sugar concentration detector, the samples of Hongyang kiwifruit in different ripening stages were expressed as different brix levels, including 6.5，10，13 and 16. By using the technique of HS-SPME/GC-MS, the flavor components of Hongyang kiwifruit with different brix values were determined. The results showed that the content of esters increased whereas the contents of alcohol and aldehyde decreased, when the brix values of kiwifruit increased. According to the analysis of Quantitative real-time PCR, the expression level of LOX family members in Hongyang kiwifruit were observed, and the results indicated that the expression of AcLox1 and AcLox5 increased but AcLox2, AcLox3, AcLox4, and AcLox6 decreased with the increasing of brix values. Overall, the results suggested that the molecular biology technology may be used as the potential approach to control the flavor of Hongyang kiwifruit and the results can provide a theoretical basis for the molecular mark assisted breeding of kiwifruit.

Abstract:In this study, response surface methodology (RSM) was performed the optimization of the fermentation of Idesia polycarpa residues, and Candida utilis was selected for solid-state fermentation. Five influences of the fermentation were studied by one-factor tests, and the Plackett-Burman experiment subsequent confirmed three main influences for Box-Behnken RSM design. Optimized conditions of the fermentation as follow: residues with ratio of solid to liquid 1:1.2, NH4NO3 additive amount 8.8%, and Candida utilis inoculation 10.5% fermented in 30℃ for 4 days. Fermentation results showed that, the crude protein content of fermented feed increased from 8.49% to 19.13%, and the nutrition ratio of products became more reasonable. This study suggests that Idesia polycarpa residues have a real potential on the modern farming by the solid-state fermentation.

Abstract:We configured a solution (TASLN) of 10 mg/mL total alkaloids of Liparis nervosa (TALN), which was extracted by Ultrasonic-assisted method. Normal saline (NS) was used as blank control and Yunan Baiyao as control in experience. For coagulation in vivo, the object was mice and we measured the bleeding time and the amount of bleeding. For coagulation in vitro, the object was rabbits. We recorded the hemostasis time of wound. the rabbits' anticoagulant blood was used to measure the coagulation time, prothrombin time (PT), Recalcification time (RT), respectively. The results showed that the time and the amount of mice bleeding was 212.95 s and 42.5 square. The coagulation time was 192.23s by blood congeals method and 245.48 s by tube method, PT and RT of rabbits' blood of TALN was 12.22 s and 180.78 s, all the times were shorter than the blank control. The average hemostasis time of total alkaloids solution of Liparis nervosa (81.50 s) was obviously shorter than that of NS (125.20 s), but showed no significant difference with that of Yunnan Baiyao (75.20 s). The TALN shows obvious coagulation activity.

Abstract:Phytoene desaturase(PDS) is the target of the bleaching herbicide,norflurazon.The the 2D and 3D structures of wild PDS and two mutations were achived by online prediction,docking of PDS and norflurazon was carried out by DOCK6.5.Binding stablity was analysed by molecular dynamics simulation via the software Gromacs.The energy changes and distribution were revealed by mm_pbsa.pl tools in Amber9.At last,important amino acids were confirmed by the analysis of hydrogen bond with LIGPLOT+. The results suggested that different mutations didn’t change the 2D and 3D structures of PDS,but mutated PDS showed higher RMSD,which indicated lower binding stablity,and higher bingding energy,which indicated the difficulity to bind to noflurazon .Our research might explain the mechanisms of the resistance to norflurazon.

Abstract:MicroRNA is a kind of small non-coding RNA, involved in plant growth and development, stress response and other physiological processes. In our previous study, we found a series of microRNA that changed expression in the stress response on the basis of gene microarray. Among them, miR 446 is particularly significant. To further explore the change and function of miRNA446 in the stress response,, we study the expression pattern of its target gene APP1 under drought and cold stress by overexpression and inhibition of miR446 in rice. The results showed that in the overexpression rice, the expression of miR446 rose after the first drop while wild-type miR446 continued to rise, and APP1 gene continue to reduce due to the splicing action of high levels miR446. In the knockout rice, the expression of miR446 was completely inhibited in the whole process, remained close to zero, and APP1 rose after the first drop in a weak trend in the stress.

Abstract:The inhibitor of succinic semialdehyde reductase AKR7A5 would be the potential drug for succinic semialdehyde dehydrogenase deficiency. The steady state kinetic mechanism of AKR7A5 and the mechanism of inhibitor interacting with the target enzyme are critical in the development of drug candidates. In spite of two crystal structures of succinic semialdehyde reductases, the details of the kinetic mechanism of AKR7A5 are unclear. Here we report the study of steady state kinetic mechanism of AKR7A5 and found the enzyme reacts in the ordered ternary complex mechanism. Based on the mechanism, we concluded that the substrate inhibition of succinic semialdehyde is caused by the wrong binding to the binary complex AKR7A5 and NADP+. The substrate analogue succinate is uncompetitive inhibitor by binding to the binary complex of AKR7A5 and NADP+. This information is necessary to obtain the ternary complex structure of AKR7A5 and succinate or other uncompetitive inhibitors, as the structural basis for inhibitor optimization.