Organizer:Ministry of Education
Governing Body:Sichuan University
Editor in chief:WANG Yu-Zhong
The standing deputy editor:ZOU Fang-Dong
ISSN:51-1595/N
Edit and Publish Editorial Department of
Journal of Sichuan University
(Natural Science Edition)
2019, 56(4):581-587.
Abstract:The chaotic characteristics of two dimensional random coupled logistic map are studied in this paper. The coupling coefficients of the system are considered to obey two points distribution,and the phase diagram and Lyapunov exponents are exploited to judge the system state. Numerical results show that, the system can lead to chaos based on periodic bifurcation and Hopf bifurcation when the coupling coefficients jump between the chaotic and non-chaotic regions according to certain probability. In particular, the probability density functions of the system orbits reveal the evolution of the system.
2019, 56(4):588-594.
Abstract:Based on the theory of Lie algebra cohomology and the definition of the Atiyah class of a Lie bialgebra, Atiyah classes of all three-dimensional Lie bialgebras over Z_3 are calculated.
2019, 56(4):595-599.
Abstract:In this paper, we study the intertwining relation of a class of integral-type operators by composition operators on logarithmic Bloch-type spaces, and give an equivalent condition.
2019, 56(4):600-606.
Abstract:By using the fixed point theorem for mixed monotone operator, a new criterion for the existence and uniqueness of positive solutions of a class of fractional impulsive differential equations with boundary conditions is established.
2019, 56(4):607-613.
Abstract:In this paper, we use global bifurcation theorem to consider the existence of positive periodic solutions of a class of first-order functional differential equations We construct the global structure of the set of positive periodic solutions of the first-order functional differential equations, and accordingly, establish some existence results of the positive periodic solutions of the first-order functional differential equations.
2019, 56(4):614-620.
Abstract:In this paper, by using the method of Schauder fixed point theorem and Krasnoselskii's fixed point theorem, the existence of positive solutions for the boundary value problem of a class of nonlinear fractional differential equations is obtianed.
2019, 56(4):621-626.
Abstract:In this paper, we study the global structure of positive solutions for a class of nonlinear second-order difference equation with three-point boundary value problems. Applying the fixed point index theory on cone and connectivity properties of the solution set, the global structure of positive solutions is obtained under the conditions that the nonlinear term satisfies the superlinear growth and the sublinear growth respectively.
Zhou Yuanling , HU XiaoBing , HUO YunLiang , ZHANG HanMing
2019, 56(4):627-632.
Abstract:Aiming at the problems of premature convergence and poor local search ability of traditional Genetic Algorithm(GA) in batch production process optimization, an Improved Genetic Algorithm(IGA) is designed by means of pretreatment technology. Random number method(RNM), rule generation method(RGM), and block gene interpolation method(BGIM) are used to initialize the population according to the appropriate ratio, which not only ensures the initial population diversity but also improves the individual quality. Elite retention strategies(ERS) and tournament selection strategies(TSS) are used to select individuals with good performance. A method is used to select crossover sites and take crossover operation based on the combination of location and priority obtained by expert marking, which preserves the superior genes and avoids “premature convergence”. The mutation operation is carried out via the neighborhood reorganization strategy(NRS) to ensure the generation of highquality solution populations and the inheritance of quality solutions. IGA regards maximizing the minimum delivery lead time as the objective function and realizes the scheduling algorithm research. Finally, the doublebase ball flat propellant production line is taken as an example to realize the scheduling process based on IGA, which greatly improved the order estimation efficiency of the company and the organization efficiency of the production line. The superiority of IGA is demonstrated by comparing both single and mixed initial population method.
2019, 56(4):633-638. DOI: 10.3969/j.issn.04906756.2019.04.009
Abstract:In streaming data, dimension reduction is an important factor in processing multidimensional sequence data. In this paper, a double transform algorithm (DTA) is proposed. For the online sequence data, unitary transformation and hyperbolic rotation transformation is carried out respectively in DTA,and the parameters of the hypothesis function are obtained. The error values are predicted by the Newton iterative algorithm, and the optimal prediction value is obtained until the error is less than the predefined threshold. The simulation results show that, compared with the two algorithms of OGD and RON, the DTA algorithm effectively reduces the computation time under the premise of ensuring the algorithm stability.
wangzhujun , zhuyingqi , sunjieping
2019, 56(4):639-644.
Abstract:It is of great significance to accurately predict the material demand of substation and distribution network for saving project cost and improving capital utilization. Researchers have carried out a series of studies on power material demand forecasting, and proposed many prediction models and algorithms such as neural networkbased algorithms. However, these algorithms have several disadvantages. Specifically, these algorithms can only process simple and ideal input, predict the demand of limited materials, and suffer from the problem of low accuracy. As a result, in current production systems, the demand of electric power materials is predicted by experienced experts according to the preliminary design scheme of the project manually. In order to solve the existing shortcomings of the current demand forecasting methods, this paper proposes a forecasting method based on matrix decomposition. The method takes the historical data of the power grid construction project material requirements and part of the project plan as input, and use matrix decomposition algorithm to predict the demand for other materials in the project. The matrix decomposition algorithm can be implemented with the material data of some projects instead of a large amount of historical usage data. In addition, the developed model does not need to be trained in advance.
zhoukun , QI Shu-nan , LI Jing , JIN Liu-Qian , CAI Ji-rong , HUANG Tian-nin
2019, 56(4):645-653.
Abstract:This paper investigates the stability analysis and stabilization for T-S fuzzy systems with time-varying delays. Firstly, an augmented Lyapunov-Krasovskii functional is constructed, delay-dependent stability criteria in terms of linear matrix inequalities (LMIs) are obtained by combining with the free-matrix-based integral inequality. Based on the state space decomposition method, some piecewise membership functions are employed to approximate the membership functions, which can be locally represented in terms of the convex combinations of the supremum and infimum of some local basis functions, the boundary information of the membership functions is taken into consideration adequately, meanwhile, some slack matrices are employed, and then some relaxed membership-function-dependent stability results are obtained. Secondly, state feedback controllers for the fuzzy systems with time-varying delays are presented under the imperfect premise matching technique, whose membership functions and the number of fuzzy rules are allowed to be designed freely, consequently, the flexibility of controller design is improved. Finally, two numerical examples are given to demonstrate the effectiveness of the presented approaches.
liukai , fangyong , zhanglei , zuozheng , liuliang
2019, 56(4):654-660.
Abstract:Many new types of malwares are often modified by attackers based on the existing malwares. Therefore, family homology analysis of malwares can help to study of evolutionary trend and traceability of malwares. In this paper, starting from API call graphs of malwares and combined with Graph Convolutional Networks (GCN), we proposed a similarity calculation and family clustering model for malwares. Firstly, we extract API call graphs of malwares with disassembly tools and the attribution of the API functions in the graphs are labeled. Then, we select key API functions by its contribution to the malware families and the API call graphs of malwares are constructed. We use GCN and Convolutional Neural Networks (CNN) as the model of the malware similarity calculation which the inputs are the API call graphs. Finally, we use DBSCAN algorithm to cluster malwares. The experimental results show that the proposed method can achieve 87.3% accuracy and can effectively cluster malware families.
2019, 56(4):661-670.
Abstract:Fully homomorphic encryption (FHE), which allows arbitrary computation of ciphertexts without knowing the secret information, has become a hot research direction in the context of big data and cloud security and has made great progress in recent years. However, in practical applications, fully homomorphic encryption still faces many problems, among which the serious expansion of ciphertext brings great pressure to the transmission of ciphertext. This problem can be effectively solved by combining the full homomorphic encryption scheme with symmetric cipher. The GSW-type full homomorphic encryption scheme is more efficient, and does not need the re-linearization technique in homomorphic evaluations. This paper selects the MGSW15 scheme which supports parallel operations, and whose ciphertext can be transformed into the ciphertext of any FHE scheme based on LWE. We present the basic framework of achieving ciphertext compression based on MGSW15 scheme in the background of cloud computing, and implement homomorphic evaluations of the block cipher AES-128, PRINCE and SIMON-64/128 circuits respectively. According to the structural characteristics of each block cipher, we use various kinds of plaintext-slicing ways to improve the efficiency of homomorphic evaluation. Finally, we analyze efficiency and security. Combined with the security and generality of AES and the efficiency of lightweight block cipher algorithms PRINCE and SIMON, the work of this paper is more efficient, and more widely used in practical applications, in which the communication complexity of ciphertexts is approximately equal to the scale of plaintexts and only one homomorphic multiplications are needed every plaintext bit.
SHI XiaoDi , LI YONGFENG , GUO QingGong
2019, 56(4):671-676.
Abstract:This paper studied the miniaturization design of an asymmetric three-coil magnetic resonant coupling wireless power transmission system for the special application of implantable medical devices, wireless sensors and portable electronic devices such as mobile phones. Based on the equivalent circuit model, the expression of the transmission efficiency of the three-coil system is deduced and the transmission characteristics of the three-coil system and the condition of the maximum efficiency transmission are analyzed. The outer diameter of the receiving coil is only 38.0 mm and the thickness is 0.6 mm. the measured transmission efficiency is 79.4% at 10mm distance, and the transmission efficiency is above 50% within 100mm distance. The effects of the axial misalignment and deflection angle on transmission efficiency are studied by simulation and experiment.
Gong Hao Ling , Zhou Xin Zhi , Ning Qian
2019, 56(4):677-683.
Abstract:Experimenting an improved A* algorithm is used to plan the mission path of uav. The uav’s step length,the restriction of turning angle and flight height are introduced to effectively reduce the uav’s search space,and constraints such as terrain and mission are introduced to make the route planning closer to the real environment. Considering the uncertainty of the enemy’s ability in the planning process and unpredictability of survival probability of the uav in the route, a markov model was used, which uses eight states markov chain to describe the state of uav. A* algorithm is combined the model in threat area after completed the task,due to the accumulation of time, the outer point’s cost of survival is higger than the inner point, it results too many iterations, but this problem can be avoided by virtue of the lower survival value of concentric circle model, therefore, introducing the united model which combined by the concentric circle model and markov model. Simulation experiment results show that the method can effectively detect route in the probability of various states of the uav and evaluate the whole route, and effectively reduce many iterations only by using the Markov model when planning new route after completing the task.
HE Han-Tao , DU Qiang , LIN Xing-De , LIU Shu-Kui , TANG Chang-Jian , XING Hao-Yang , YUE Qian , ZHU Jing-Jun
2019, 56(4):684-688.
Abstract:In the dark matter direct detection experiment, neutrons are important environmental background. In the China Jinping Underground Laboratory (CJPL), the world’s deepest underground laboratory, a gadolinium doped liquid scintillator (Gd-LS) detector was used to measure the fluxes and spectra of fast neutron background in the Hall and in a room with one meter thick polyethylene (PE) walls by fast-slow time coincident method. Liquid scintillators are incapable to discriminate neutrons and alpha-particles by their pulse shapes. Thus, the alpha-particle background from the long-lived U/Th nuclides inside the liquid scintillator has to be taken into account. Assuming secular equilibrium, the total rate of the alpha-decays in the detector was measured to be (0.548 ± 0.002) s-1. Then in the Hall, the number of random coincident alpha-events was determined to be 6 out of 2682 neutron candidates based on 356 days of data. And for the PE room, this value is 2 out of 44 neutron candidates with 173 days of data. The influence of the random coincident alpha-particles are negligible for the result of fast neutron flux in the Hall, but have around 4% for the measurement in the PE room.
YI Li-Li , MAO Zhong-Fa , ZHANG Zheng-Wen
2019, 56(4):689-694.
Abstract:In view of the micro-mechanical property of copper alloy fabricated by selective laser melting (SLM), the effects of different processing parameters (laser power, scanning speed and scanning space) on the nanoindentation hardness were researched according to the orthogonal experimental design test method. Based on the analysis of variance, the impact degree of diverse processing parameters on the nanohardness was obtained, which could provide a theoretical direction for improving the micro-mechanical property of parts during SLM.
ZHANG Qing-Biao , ZHANG Zhi-Xiong , TANG Li , LIANG Ke-Shan , ZI Fei-Yue
2019, 56(4):695-702.
Abstract:Lightweight sandwich structures with high strength and high energy absorption ability are of paramount importance in the mechanical field. An elastic damping unit structure based on SLM (laser selective melting) technology is proposed, which can generate large elastic deformation when loaded, so that a large amount of energy is absorbed in the elastic deformation stage. According to the characteristics of SLM process, the structure and layout of the elastic damping unit are designed, and the manufacturability constraints are studied. Finally, three sets of elastic damping units with different structural parameters and the Kagome structure with the same height were processed by SLM technology, and the compression test was carried out. According to the test results, the influence of unit structure parameters on its bearing capacity and energy absorption capacity was analyzed, and the difference of elastic damping unit and Kagome structure performance was compared. The test results show that the strength and energy absorption capacity of elastic damping unit are better than Kagome, and when the parameters are a=2.2 mm, m=3.1 mm, n=4.6 mm, at the same weight, the strength of the elastic damping unit is about 36.11% higher than that of the Kagome structure, the energy absorbed at the time of failure is about 26.83% higher than that of the Kagome structure, and the energy absorbed during elastic deformation is about 39.1% higher than that of the Kagome structure.
GAO Xin-Xin , HUA Wei , ZHANG Hong , CHANG Ying
2019, 56(4):703-708.
Abstract:A 3-D model of microwave argon plasma (Ar-MIP) under atmospheric pressure is established with the finite element method to describe the temporal and spatial distribution of plasma temperature, pressure, and velocity. The interaction of these parameters is studied to explore the mechanism of microwave plasma excitation. The results show that the plasma temperature increases upon increasing the time, and the high temperature distribution exhibits spatial inhomogeneity, which causes the local changes in gas pressure and affects the flow velocity leading the thermal flow around the heat area. This is the reason that the fluid in high temperature region slows down.
YUAN Guang-Hui , WANG Bing , TAO Yang
2019, 56(4):709-712.
Abstract:Low-density foams have important applications in high-energy density physics, radiation transport and adiabatic molding. However, it is very difficult to make these materials into tiny parts for their porous and fragile. Therefore, two special diamond tools were designed and produced, which were used for turning of cylindrical surface and cutting. Kinds of low-density CH foam micro-column used for ICF were machined by precision turning micromachining technology. The effects of cutting conditions, geometry parameters of diamond tool and machining parameters on the forming of CH foam micro-column were discussed in detail.
2019, 56(4):713-716.
Abstract:The generations of the even-order harmonics from vibrating H2+ have been theoretically investigated and identified. The results showed that due to asymmetric harmonic emission (asymmetric half-wave profile) on the rising and the falling parts of the laser field, the even-order harmonics can be generated. In detail, for the case of the lower initial vibrational state, the even-order harmonics mainly come from the falling part of the laser field; while as the initial vibrational state increases, the identified even-order harmonics on the falling part of the laser field decreases; while some other even-order harmonics coming from the rising part of the laser field are gradually enhanced.
LI Xu-Sheng , HAN Xu , YAN Pei , ZHU Chao-Jie
2019, 56(4):717-720.
Abstract:In this paper, based on the first-principles method the physical parameters such as lattice constants and bulk moduli of water molecules clathrate and methane hydrate with s-III type are studied. Both the two cage structures can be stable in the pressure range of 0-2.6GPa, and the volume decreases approximately linearly with the increasing pressure. The volume of methane hydrate with the s-III type expands due to the interactions between CH4 molecules, but the increase of volume is smaller and uniform with an average increase magnitude of about 3.97%. Finally, it is found that the methane hydrate with s-III type is an insulator and not conductive according to the calculated band structure and electronic density of states of methane hydrate with s-III type.
SUN Jie , FANG Hong-Jie , ZHAO Jun-Feng , DAI Han , LÜ Zheng-Feng , WANG Hong-Bo
2019, 56(4):721-726.
Abstract:The effect of catechol as chelating agent is used to study the structures and photoluminescence of ZnO one dimensional rod like materials by hydrothermal methods. We find that the morphologies and length to diameter ratio are depended on the concentration of catechol. The XRD test show that the crystallization degree of ZnO one dimensional rod like materials increases remarkably with the addition of catechol. By increasing the amount of catechol, the ZnO crystal exhibits preferential growth along (100) direction. When the amount of catechol reaches 1g/L, the crystalline degree of ZnO crystal shows the highest degree of crystallization. In addition, the photoluminescence test shows that ZnO one dimensional rod like materials exhibits strong photoluminescence enhancement around UV-vis spectroscopy. In combination with morphology and phase analysis, the influences of the crystallization and surface defects of ZnO materials on the photoluminescence enhancement are revealed. This work reveals the chelating effect of catechol on the growth of ZnO materials and improves the photoluminescence properties of ZnO by modulating the quantity of catechol.
XIN Jin-Peng , WU Yu-Ting , LI Song , LI Jie , DAI Wei
2019, 56(4):727-734.
Abstract:The method of grand canonical Monte Carlo has been applied to study the adsorption behavior of hydrogen in two kinds of Metal Organic Frameworks (MOFs) , RE-shp-MOF-1 and RE–alb-MOF-1.Their pore size distributions are analyzed by the model of Non Local Density Functional Theory(NLDFT).The results revealthatRE-shp-MOF-1 and RE-alb-MOF-1 both behave good hydrogen storage properties in the condition of temperature at 77 K and pressure at 1000 kPa. The hydrogen storage capacities can meet the requirements of the U.S. Department of Energy (DOE).There are two plateaus on adsorption isotherm due to the evident difference between their pore sizes. Eventually, RE-shp-MOF-1 behaves better hydrogen storage properties and consequently suit to be used as ideal hydrogen storage materials due to a number of advantages over RE-alb-MOF-1,such as more narrower pore with size of 10~20 Å, larger specific surface area and pore volume.
LI Zong-Bao , WANG Xia , SHI Wei
2019, 56(4):735-742.
Abstract:It becomes an effective way to solve environmental pollution by using photocatalysis of semiconductors. For the reasons of chemical stabilization and unique electronic structure, polymeric graphitic carbon nitride (g-C3N4) has been used extensively in the field of photocatalysis. Using the first principle calculations, optimized crystal structures and electronic structures are calculated for the doped cases. From comparisons of formation energies, the most stable crystal structures are obtained for monatomic substitutional doping cases and polyatomic surface transfer doping ones. The results reveal that C/g-C3N4 can be easier synthetized than N/g-C3N4 because of its lower formation energy. The results show that the best doping ratio of C is 1/12 for the reason of high visible light response. The calculated results are helpful to provide theoretical basis and instruction for subsequent synthesis in addition to the microscopic mechanism of doping.
LI Xiao-Jie , MIAO Hui , WANG Di , ZHAN Hao-Miao , WANG Lin-Lin , WU Chuan-Fang
2019, 56(4):743-748.
Abstract:To explore the role of RNA secondary structure in the interaction between RNA and SAFB1 protein, structure analysis and RNA footprinting analysis were adopted. It revealed that SAFB1 binds to a specific region of LINC-PINT-1 and the SAFB1 binding region has a specific secondary structure. In addition, subcloning of LINC-PINT-1 confirmed that subclones contained the 30~55 nt region could maintain this secondary structure. Electrophoretic mobility shift assay(EMSA) found that this secondary structure was involved in the interaction between RNA and SAFB1. In this paper, software prediction and necessary experimental verification have indicated that the interaction between LINC-PINT-1 and SAFB1 depends on the specific RNA secondary structure, which provides a powerful theoretical experimental support for the study of the molecular mechanism of RNAprotein interaction.
XU Zhen-Peng , QIU Long , YANG Ting , LIN Hong-Hui , XI De-Hui
2019, 56(4):749-757.
Abstract:To explore the interaction between temperature as environmental factor and defense mechanism in the plant, we designed the experiment to research the action of Nicotiana benthamiana (Nb)infected Turnip mosaic virus (TuMV)under different temperature conditions, to analyze the effect of temperature to viral defense mechanism in the plant through a series of techniques of molecular biology. The analysis of Western blotting indicated that the infection of TuMV was reduced at a high temperature (30°C) compared with those under normal temperature (23°C) conditions. Moreover, salicylate hydroxylase (NahG)transgenic N. benthamiana plants exhibited higher resistance to TuMV at high temperatures than the wild type plants. Further experiments of enzymelinked immunosorbent assay showed higher activities for some antioxidant enzymes and higher reduced glutathione contents in the NahG plants at high temperature. The regulated jasmonic acid (JA) gene exhibited high expression under high temperature conditions at the late stage of infection. In addition, key components of the RNA silencing pathway were upregulated in the NahG plants. Taken together, these results indicated that high temperature had significant effects on plant resistance and improved the tolerance of N. benthamiana plants against TuMV infection via RNA silencing and related phytohormone pathways.
BAI Yan , FENG Wen-Long , ZHANG Zong-Jin , DENG Li-Yu , Gu yong , LI Bin , CHEN Qiang , ZHANG Xiao-Ping , GU Yun-Fu
2019, 56(4):758-764.
Abstract:To study the diversity of phyllospheric bacteria cultured in flue-cured tobacco leaves, and explore the resource of phyllospheric bacteria, this research collected flue-cured tobacco leaves in their fast growing stage from different traditional cultivation areas of Sichuan province, China. Phyllospheric bacteria strains were isolated by pure culture method, and their genetic diversity and functional characteristics were studied via determination of strains of indole 3-acetic acid (IAA) production ability, phosphorus and potassium solubilizing activities, cellulose degradation activity and antibacterial ability, as well as by transcription factor amplification technology (BOXA1R-PCR), 16S rRNA gene sequencing and phylogeny analysis. The results showed that among the 86 isolates, 13.95% (12/86) of the strains had the ability to produce IAA, 4of them had a higher yield (>57μg/mL); 10.47% (9/86) had phosphate solubilizing activities; 9.30% (8/86) showed solubilizing potassium activities. Based on the BOXA1R-PCR patterns, 12 representative strains were selected for 16S rRNA gene sequencing. Phylogenetic analysis based on the 16S rRNA gene sequencing showed that the isolates belonged to Arthrobacter, Lysinibacillus, Stenotrophomonas, Achromobacter, and Pseudomona, in which Pseudomona was the dominant genus. All results indicated that abundant culturable phyllospheric bacteria existed in flue-cured tobacco leaves in traditional tobacco areas in Sichuan province.
SONG Ji-Ling , LU Na , WANG Wei-Ke , YUAN Wei-Dong , LI Hai-Bo , CHENG Jun-Wen , KANG Xue-Ping , YAN Jing
2019, 56(4):765-770.
Abstract:For accurate identification of three relative Sanghuangporus species including S. sanghuang, S.vaninii and S. baumii, the phylogenetic analysis and molecular recognition of relative Sanghuangporus species were performed based on the internal transcribed spacer (ITS) sequence of the ribosome rDNA. NJ phylogenetic tree indicated that 23 S.sanghuang strains, 11 S. vaninii strains and 6 S. baumii strains formed three independent clades with a high bootstrap support rate, and the obvious differences among the three relative species were presented. Based on the difference in rDNA ITS sequence alignment of S.sanghuang and S.vaninii, two pairs of primers, Sv_U1/Sv_L and Sv_U2/Sv_L, were designed, which can specifically amplify two ITS fragments of 478 bp and 651 bp only from S.vaninii. but not from S. sanghuang, indicating that this method can be effectively used for the molecular recognition of the two relative Sanghuangporus species. This study provides the evidence for the phylogenetic relationship of Sanghuangporus genus, and an effective molecular assisted tool for the precise recognition of relative Sanghuangporus species.
WANG Qiao , ZHANG Yu-Jun , JIANG Xian-Jie
2019, 56(4):771-778.
Abstract:Our experimental data indicate that overexpression of p80 in p80 null T cell leukemia cells significantly down regulates c-Myc oncoprotein and blocks cell cycle progression from G1 to S phase. The qPCR examination and protein stability test found that p80 regulates expression of c-Myc at the transcriptional level. The analysis of c-Myc promoter activity at the presence of p80 by Dual Luciferase Reporter Assay indicated that the transcriptional repression of p80 on c-Myc promoter is located at -1042bp~-630bp above the transcription start site of cMyc gene. The transcription factor binding sites search of this region by software TFSEARCH revealed multiple binding sequences of c-Myb, another oncoprotein and transcription factor which plays an important role in the proliferation and differentiation of blood cells during hematopoiesis. Knockdown c-Myb in MOLT-4 cells stably expressing p80 indicate that p80 suppresses the transcription of c-Myc gene through c-Myb. Co-immunoprecipitation experiment demonstrated that p80 interacts with c-Myb inside cells. The further experiment has shown that transcriptional repression of c-Myc by p80 is dependent on the enzymatic activity of histone deacetylase. Taken together, our results suggest that p80 recruits histone deacetylase to the c-Myc promoter through interaction with transcription factor c-Myb, and p80 suppresses c-Myc expression by histone deacetylation.
PENG Tian-Xiang , DAI Jiao , CHEN Fang , XU Ying
2019, 56(4):779-784.
Abstract:In order to overcome the shortcomings of the existing propagation technology of Jatropha curcas, adventitious buds were induced from the adventitious root explants of cotyledon and radicle explants respectively. The optimal way to get adventitious root from cotyledons of Jatropha curcas L. was that it would be precultured on the MS medium containing 5 mg/L IBA for 4 days, then transferred to MS0 medium for rooting. The rooting inducing rate and adventitious root number reached 93.33% and 6.54/explant, respectively. The optimal medium for adventitious buds inducing was MS+3.0 mg/L 6-BA+3 mg/L IAA. The induction rate could reach 83.33%. The optimal adventitious bud induction medium from the radicle explants of Jatropha curcas L. was MS+0.2 mg/L 6-BA+1.0 mg/L IBA. The differentiation rate was 67.80%, and the average number of sprouts was 3.25 per explant. Paraffin sections were observed at each stage of bud regeneration and it seems that adventitious buds originate from parenchyma of roots. Compared with the prior methodology, the method presented here has the characteristics of a shorter cycle and higher regeneration efficiency.
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