Synthesis and evaluation of near infrared fluorescent dye BPAD-2 for β-amyloid plaques
DOI:
Author:
Affiliation:

Clc Number:

Q

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
    Abstract:

    OBJECTIVE We synthesized a benzopyrane derivative BPAD-2 with push-pull electronic structure, and assessed its fluorescence properties, labelling ability for beta-amyloid plaques, and blood-brain barrier penetrating ability. METHODS BPAD-2 was synthesized by condensation reaction with 1-(2-hydroxy-phenyl)-ethanone as the primary reagent, and confirmed by 1H-NMR and MS. Fluorescence spectra were observed; fluorescence staining was performed to evaluate labelling ability for beta-amyloid plaques; fluorescent imaging was performed to evaluate blood-brain barrier penetrating ability. RESULTS The emission maximum of BPAD-2 in PBS was at 658 nm; BPAD-2 showed bright red labelling of beta-amyloid plaques; BPAD-2 penetrated the blood-brain barrier and peaked at 10 min postinjection and showed reasonable washout from the brain. CONCLSION BPAD-2 showed specific labelling of beta-amyloid plaques and had good blood-brain barrier penetrating ability.

    Reference
    Related
    Cited by
Get Citation

Cite this article as: CHENG Yan, OUYANG Xiang-Shuo, LIANG Tian, ZHANG Zhi-Rong. Synthesis and evaluation of near infrared fluorescent dye BPAD-2 for β-amyloid plaques [J]. J Sichuan Univ: Nat Sci Ed, 2016, 53: 193.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:June 20,2014
  • Revised:October 08,2015
  • Adopted:October 09,2015
  • Online: May 30,2016
  • Published: