Screening of Phospholipase D producing Strains and its transphosphatidylation activity
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Q939.99

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    Abstract:

    Using soybean Phospholipids as substrates,one strain which expressed Phospholipase was isolated from soil near oil mill ,the Phospholipase produce by SNUPLD-6 was identified which contain Phospholipase D . According to its mor -phological characteristics and ITS sequence , The strain SNUPLD-6 was identified as Geotrichum candidum. study on the Conditions for the growth of the bacteria and the PLD reaction conditions which expressed by SNUPLD-6. The results showed that its optimum growthtemperature is 30℃ .its optimum growth pH value is 6.0,its optimum carbon source is glucose,its optimum nitrogen source for the beef extract and peptone 50% each. the optimal fermentation time of phospholipase D producing is 3d, its optimum transphosphatidylation-temperature is 32℃. its optimum transphosphatidylation pH value is 6.0,its optimum reaction buffer for pH=5.5 0.02M HAc -NaAC buffer, its Optimal metalion activator as Ca2 +, Zn2 +.

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Cite this article as: SHI Chuang, WANG Yi-Ding, MA Qin-Qin, YUAN Xiang-Hua. Screening of Phospholipase D producing Strains and its transphosphatidylation activity [J]. J Sichuan Univ: Nat Sci Ed, 2016, 53: 215.

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History
  • Received:November 21,2014
  • Revised:May 28,2015
  • Adopted:June 21,2015
  • Online: May 30,2016
  • Published: