Antifugal activity and GC/MS analysis of extracts from Curcuma phaeocaulis stems and leaves against plant pathogenic fungi

Abstract:

To not only compare the in vitro antifungal activities against phytopathogens between ethanol and n-Hexane extracts of the stems and leaves from C. phaeocaulis, but also the differences of their active chemical components. The ethanol extractum of the stems and leaves from C. phaeocaulis had been prepared, then it was not only extracted by n-Hexane, ethylacetate, and methanol, but also the ethanol and n-Hexane extracts from stems and leaves were chemically analyzed by GC/MS. Results indicated that both the ethanol and n-Hexane extract showed well antifungal activity against pathogenic fungi including Fusarium graminearum, Phoma wasabiae, Sclerotinia sclerotiorum and Botrytis cinerea. Relative antifungal activities of three extracts, were compared and the yield of n-Hexane was 64.68%, higher than those of others while its antifungal activity was also the highest. The GC/MS results showed that there were 11 components increased but 23 components decreased in concentrations, and the highest components were curcumenol (24.46%) and hexadecanoic acid, methyl ester (13.84%) in n-Hexane extract. There were 28 components in ethanol extractum and 23 ones in n-Hexane extractum identified by GC/MS analysis.