Abstract:BnTR1 is an E3 ligase localized in the plasma membrane, and the previous studies indicated that it played a key role in heat stress response in Brassica nupus, rice and E. coli. In order to investigate the comprehensive resistance characteristic of B.napus over-expressing BnTR1 lines, this research designed a variety of stress conditions to observe and analysis their phenotype. Results showed that compared with the control, the transgenic plant showed a better resistance to hydrogen peroxide, salt, and mannitol. In addition, the activity of superoxide dismutase(SOD),peroxidas (POD) and catalase(CAT) in transgenic plant was higher than the control under salt stress. These results confirmed that the over expression of BnTR1 enhanced the tolerance of Brassica nupus against a variety of stress, which laid a foundation for further research of the function of BnTR1, also provided original materials for breeding new varieties.
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