Investigation and co-transfer of resistance genes and virulence genes among Escherichia coli producing CMY-2 isolated from feces and flies among large-scale poultry farms
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    Abstract:

    The prevalence and co-transfer of resistance genes and virulence genes among Escherichia coli producing CMY-2 from feces and flies among twenty large-scale poultry farms in China were investigated. Polymerase chain reaction (PCR) was used to examine for the presence of E.coli producing CMY-2. The genetic relationship between isolates carrying blaCMY-2 gene was determined by pulsed-field gel electrophoresis (PFGE). Antimicrobial susceptibilities to 13 antimicrobials agents of isolates producing CMY-2 were tested by diffusion methods. Characterization of isolates producing CMY-2, including other resistance genes, and virulence genes was detected by PCR. The transmission of resistance and virulence genes was demonstrated by conjugation experiment, PCR-based replicon typing (PBRT). The results showed that 33 isolates were confirmed to carry blaCMY-2 gene with a positive rate of 6.0%, which showed resistance to multiple antimicrobial agents. Fifteen resistance genes conferring resistance to other antimicrobial agents were identified, including blaTEM-1, blaCTX-M-55, blaOXA-1, qnrBDS, aac(6’)-Ib-cr, oqxA, tetA, tetC, sul1, sul2, sul3, rmtB, and flor. Furthermore, four virulence genes including iutA, traT, VagC, and fyuA gene were also detected. Twenty-one transconjuncgants were detected in 33 E.coli Producing CMY-2. The co-transfer of between resistance genes and virulence genes was observed on transferred plasmids of lncA/C or lncI1. This study confirmed the prevalence and co-transfer of resistance genes and virulence genes in isolates of E.coli producing CMY-2, providing a reference for disease prevention and transmission in poultry farms.

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Cite this article as: LI Qing-Zhou, MA Su-Zhen, KONG Ling-Han, JIANG Wei, WANG Hong-Ning. Investigation and co-transfer of resistance genes and virulence genes among Escherichia coli producing CMY-2 isolated from feces and flies among large-scale poultry farms [J]. J Sichuan Univ: Nat Sci Ed, 2017, 54: 417.

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History
  • Received:March 25,2016
  • Revised:April 12,2016
  • Adopted:April 29,2016
  • Online: April 08,2017
  • Published: