The expression of long non-coding RNA LINC00941 in colorectal cancer and effect on cell proliferation

Abstract:

To explore the function of a cell differentiation-associated long non-coding RNA (lncRNA) LINC00941 in cancer progression, we detected the expression of LINC00941 in 6 types of human cancer cell lines and HEK-293 cell line by real-time quantitative PCR. LINC00941 is highly expressed in colorectal carcinoma cell lines HCT116 and HCT116 p53-/-, lung carcinoma cell lines A549 and NCI-H1299, and melanoma cell line Stilling, especially in colorectal carcinoma cell lines. By detecting the RNA levels of LINC00941 in the tumor and normal tissues of colorectal cancer patients, we found that the expression levels were significantly increased in tumor compared with paired normal tissues. By shRNA(short hairpin RNA) interfering, we found LINC00941-knockdown HCT116 cells had lower proliferation rate compared with negative control cells, suggesting the correlation of LINC00941 and colorectal cancer progression.