cDNA cloning of heat shock protein 60 (SpHsp60) in Stegobium paniceum and its expression under temperature stress

Abstract:

The full-length cDNA sequence of a heat shock protein 60 (Hsp60) gene from Stegobium paniceum (a major insect pest in stored Chinese medicine material) was cloned by using RT-PCR technique, and termed as SpHsp60 (GenBank accession no. KX778623). Sequence analysis showed that SpHsp60 contained an open reading frame of 1719 bp encoding 572 amino acids, with a predicated molecular weight of 60.9 kDa and isoelectric point of 5.32. SpHsp60 had the highly conserved functional motifs of the Hsp60 gene family, and SpHsp60 shares 88% amino acid sequence identities with the Hsp60 proteins from Tribolium castaneum and Leptinotarsa decemlineata. The quantitative real-time PCR results indicated that the expression of SpHsp60 was significantly increased 2 hours after exposed to -5°C and 0°C, compared to the control, and this gene was also induced after exposed to 42°C. The results suggest that the SpHsp60 gene may correlate with extreme temperature-stress tolerance in S. paniceum.