ISSR analysis of the genetic diversity of Artocarpus nanchuanensis， a critically endangered species, in Nanchuan District, Chongqing

Abstract:

Genetic diversity of 46 strainswild Artocarpus nanchuanensis from seven areas of Chongqing was preliminary analyzed using inter-simple sequence repeat (ISSR) PCR molecular marker technology. The selected 12 pairs of UBC primers were used in this research, and a total of 117 DNA fragments were obtained, of which 113 were polymorphic fragments, and the percentage of polymorphic bands was 96.58%. The analysis by NTSYS pc2.1 software showed that the genetic similarity among Artocarpus nanchuanensis from the seven areas was ranged from 0.062~0.989, and the dendrogram was constructed through UPGMA method. The results revealed that these Artocarpus heterophyllus Lam. have a similar genetic background with obvious differences. These Artocarpus heterophyllus Lam. were obviously divided into four groups, and the cluster is in line with the similar geographical origin of materials, showing a certain regional distribution.