Cordyceps cicadae extracts has a protect effect against H2O2-induced cellular senescence by promoting oxidative stress response in HeLa cells

Abstract:

This study aims to investigate the protective role of Cordyceps cicadae extracts(CCE)against H2O2induced oxidative stress in HeLa cells.HeLa cells were treated with CCE for 48h and 72 h to check cell viability.The SAβGaL activity and intracellular ROS was detected under the microscope after H2O2 treatment.The mRNA levels of CAT、SOD1、SOD2 and GPX1 were measured by real time PCR in HeLa cells after CCE treatment.Results show that CCE inhibited the growth of HeLa cells in a dosedependent manner.Pretreated HeLa cells with CCE (0.100mg/mL) showed decrease cellular senescence and level of ROS against H2O2induced oxidative stress.CCE can increase CAT and SOD1.Our results indicate that the CCE has protective effect against H2O2induced cellular senescence in HeLa cells.