The characteristic of transgene insertion and exogenous protein expression in Chlamydomonas reinhardtii
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Q943.2

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    Transformation of the cell wall deficient C.reinhardtii CC-400 with the plasmid pSP108 was carried out. Adapter PCR analysis on flanking sequences of ble in positive transformants showed that: the foreign gene ble inserted randomly, plasmid sequences rearranged with nuclear genome sequences and fracture phenomenon occurred. ELISA was used to analyze the expression of foreign gene, combined with the gene insertion which indicated that: In the positive transformants of which promote is partially truncated, the promoter in the unknown gene can initiate the expression, but the level is lower than the RBCS2 promoter initiation; In the other transfomants of which the promoter is complete, the protein expression level is low, may be due to the lacking of partial C.reinhardtii genome.

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Cite this article as: LIU Rui-Jun, DING Tao, PENG Si-Lun, BAI Lin-Han. The characteristic of transgene insertion and exogenous protein expression in Chlamydomonas reinhardtii [J]. J Sichuan Univ: Nat Sci Ed, 2018, 55: 655.

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History
  • Received:April 29,2017
  • Revised:May 09,2017
  • Adopted:May 10,2017
  • Online: June 06,2018
  • Published: