Macroporous adsorption resin for the purification of polysaccharides from Gastrodia elata Bl

Abstract:

The Gastrodia elata polysaccharides were purified using a macroporous adsorption resins method. By comparing the Gastrodia elata polysaccharides separation effect of eight different types （AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7）of macroporous resin, D101 was selected as the ideal adsorbent as it had a strong adsorption ability and a high desorption rate. The optimal purification technology was as follows: loading rate1 BV/h, loading concentration 4 mg/mL, 60% ethanol as suitable elution solvent from the resin, the elution solvent volume 3 BV and the elution flow rate 2 BV/h. The purity of Gastrodia elata polysaccharide was increased from 15.6% to 65.7%, which indicated that macroporous resin D101 had better purification effect on Gastrodia elata polysaccharide.