Association analysis among genetic diversity, agronomic characteristics and SSR markers in rice

Abstract:

In order to provide a basis for hybridization combination of parent materials and molecular assisted breeding, the genetic diversity of parent materials was analyzed by using SSR markers and their association with some agronomic traits. The genetic diversity analysis and population genetic structure analysis of 190 rice materials were analyzed using 60 pairs of SSR markers distributed in 12 chromosomes of rice. On this basis, the correlation analysis between the marker and agronomic traits was carried out using GLM and MLM of Tassel 3.0. The results showed that the genetic similarity coefficient (GS) variation of 190 rice materials ranged from 0.62 to 0.97 with an average of 0.86. According to the genetic structure of the opulation, the tested materials could be divided into three subgroups. With GLM analysis, 8 markers associated with spike length, a branch number, primary branch grain number, secondary branch number, secondary branch grain number, the total number of grains/ear and not full grain associated tag were found, and the tag on the interpretation of the phenotypic variation rate was 0.0648. In the MLM system analysis, the explanatory rates of phenotypic variation by 8 markers ranged from 0.0378 to 0.0648. The molecular markers lated to these eight agronomic traits obtained in this study will provide a theoretical basis for markerassisted breeding of high yielding rice varieties.