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Cite this article as CIREN Qu-Zong,LUO Yu,QU Xiao-Yu,DENG Xiao-Kuan,WANG Yong-Li,GAO Ping. Study on chemical constituents and determination of cannabidiol (CBD) in hemp leaves of Heilongjiang province[J]. J Sichuan Univ: Nat Sci Ed, 2019, 56: 957~962.
Study on chemical constituents and determination of cannabidiol (CBD) in hemp leaves of Heilongjiang province
Hits 531  Download times 160  Received:January 16, 2019  Revised:May 12, 2019
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Key Words   Hemp leaves  Isolation and purification  Structure identification  TLC  HPLC
Author NameAffiliationE-mail
CIREN Qu-Zong Key Labortatory Bio-resources and Eco-environment of Ministry Education, College of Life Sciences, Sichuan University 2426189637@qq.com 
LUO Yu Key Labortatory Bio-resources and Eco-environment of Ministry Education, College of Life Sciences, Sichuan University  
QU Xiao-Yu Key Labortatory Bio-resources and Eco-environment of Ministry Education, College of Life Sciences, Sichuan University  
DENG Xiao-Kuan Key Labortatory Bio-resources and Eco-environment of Ministry Education, College of Life Sciences, Sichuan University  
WANG Yong-Li Heilongjiang Kangyuan Biotechnology Do.Ltd  
GAO Ping Key Labortatory Bio-resources and Eco-environment of Ministry Education, College of Life Sciences, Sichuan University  
Abstract
    The purpose of this research is to study the chemical constituents in the leaves of Heilongjiang han hemp and establish a method for the determination of cannabidiol (CBD). The ethyl acetate extract of hemp leaf was separated and purified by silica gel column chromatography, C18 reverse phase medium voltage column and semi preparative chromatography to obtain 5 monomer compounds. And the structure of these compounds was determined by nuclear magnetic (NMR) analysis. They were cannabidiol (CBD), cannabigerol (CBG), cannabinol (CBN), 9-Tetrahydrocannabinolic acid (9-THCA) and cannabichromene (CBC). By using petroleum ether dichloromethane (11) as the developing solvent, iodine and 1% Vanillin ethanol sulfuric acid (101)were used to establish the TLC method for CBD respectively. High performance liquid chromatography (HPLC) detection method for CBD was also established by using acetonitrile 0.05% phosphoric acid solution (8020) as mobile phase and selecting the detection wavelength with high CBD absorption peak, which has a good linear relationship at 3.125~100g/ mL (R2=0.9997), the average recovery rate is 103.69% and RSD is 1.32% (n=5).

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