Abstract:In order to provide new ideas for the transformation of Bacillus pumilus and increase the yield of alkaline protease, in our previous studies, transcriptome of Bacillus pumilus was analysed based on RNA-seq and sRNAs were predicted. In this study, a new sRNA Bpsr112 was identified by the bioinformatics analysis and northern blot.Then the knockout and overexpression strains of Bpsr112 were constructed, and the growth curve, salt stress and protease activity were compared among them. The results showed that, compared with the control strains at 60h and 72h, the protease activities of knockout strains were significantly decreased (P<0.01), while the protease activities of overexpression strains were significantly increased (P<0.01). At the same time, SDS-PAGE results showed that the content of AprE protein decreased in knockout strains and increased in overexpression strains. These results indicated that Bpsr112 may has positive regulation on the protease activity of Bacillus pumilus.In this study, sRNA in Bacillus pumilus was identified, and Bpsr112 was found to promote protease activity.
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