Study on the expression patterns of two ribosome inactivated protein genes Curcin and Curcin C of Jatropha curcas during the process of seed germination

Abstract:

In order to research and develop the abundant resources of Jatropha curcas, the seeds of Jatropha curcas from the dry hot valley of Jinsha River in Xichang City, Sichuan Province were used as materials. The tissues for experiment were collected after soaking seeds in water to germinate. The qRTPCR results showed that the expression of Curcin and Curcin C genes in endosperm increased first and then decreased, as did the expression of Curcin C gene in cotyledons. Western Blot results showed that Curcin protein was not expressed in roots, hypocotyls, true leaves and cotyledons, Curcin C protein was also not expressed in roots and hypocotyls, but expressed in true leaves and cotyledons. Both bioinformatics software prediction and subcellular localization results showed that Curcin:GFP and CurcinC:GFP fusion proteins were localized in the cell wall. In this study, the expression patterns of two ribosome-inactivated proteins Curcin and Curcin C in Jatropha curcas were preliminarily elucidated and subcellular localization was performed.