On the interaction of 1,4,7-Triazacyclononan -Co(Ⅱ)with Bovine Serum Albumin

Abstract:

The interaction of 1,4,7-Triazacyclononan -Co(Ⅱ) (Cyclen-Co(Ⅱ))with bovine serum albumin (BSA) has been investigated by fluorescence method. The results show that the Cyclen-Co(Ⅱ) strongly quenched fluorescence of BSA. It was proved that the fluorescence quenching mechanism belonged to the static quenching by Stern-Volmer equation. According to Lineweaver-Burk equation and thermodynamics equation, the binding constants,binding sates and thermodynamics parameters were obtained at different temperatures in the aqueous solution and in the solutions of Brij35、CTAB、SDS. The hydrophobic interaction forces played a main role in the binding of Cyclen-Co(Ⅱ) with BSA.