枸杞抗坏血酸氧化酶基因的克隆与表达分析

Molecular Cloning and Expression Patterns of LcAO from Lycium Chinens

摘要:

以枸杞为材料，利用同源克隆技术，克隆了枸杞抗坏血酸氧化酶（AO）的全长 cDNA序列，命名为 LcAO 基因（GenBank：KP712033），该基因开放阅读框（ORF）大小为1737 bp，编码 578 个氨基酸，与西红柿 AO 蛋白的同源性达到 90％。LcAO 编码的氨基酸序列包含 3 个多铜氧化酶结构域和跨膜信号序列。采用实时荧光定量 PCR 分析显示，LcAO 基因在枸杞的花和果实中表达量最高，成熟叶中表达量最少。

Abstract:

A ascorbate oxidase (AO) gene from Lycium Chinense was cloned with homologous method. The full-length cDNA of Lycium Chinense AO which was named LcAO (Genbank: KP712033) was 1737 bp，and contained a complete open reading frame (ORF) of 578 amino acid residues. Homology analysis indicated that the deduced LcAO protein was highly 90% to AO proteins from Solanum lycopersicum. The results from Real-Time PCR indicated that the expression of LcAO gene was the strongest in the flower and friut, and the least in mature-leaf of Lycium Chinense.