磷脂酶D产生菌株的筛选及其转磷脂化反应特性

Screening of Phospholipase D producing Strains and its transphosphatidylation activity

摘要:

采用磷脂平板筛选法，从榨油坊附近的土样中筛选到1株能够降解磷脂的菌株SNUPLD-6，实验表明SNUPLD-6产的磷脂酶含有磷脂酶D（PLD）.经细菌形态特及ITS序列分析，鉴定其为地霉属的白地霉（Geotrichum candidum）.对该菌的生长条件及该菌产的PLD的反应条件进行研究，实验结果表明：该菌株的最适生长温度为30℃，最适PH为6，最适碳源为葡萄糖，最适氮源为牛肉膏和蛋白胨各50%.该菌产PLD最适发酵时间为3d，为胞外酶，该PLD的转磷脂化反应最适反应温度为32℃，最适pH为5.5，最适的反应缓冲液为pH为5.5的0.02M的醋酸-醋酸钠缓冲液，最适的金属离子激活剂为Ca2+，Zn2+.

Abstract:

Using soybean Phospholipids as substrates，one strain which expressed Phospholipase was isolated from soil near oil mill ，the Phospholipase produce by SNUPLD-6 was identified which contain Phospholipase D . According to its mor -phological characteristics and ITS sequence , The strain SNUPLD-6 was identified as Geotrichum candidum. study on the Conditions for the growth of the bacteria and the PLD reaction conditions which expressed by SNUPLD-6. The results showed that its optimum growthtemperature is 30℃ .its optimum growth pH value is 6.0，its optimum carbon source is glucose，its optimum nitrogen source for the beef extract and peptone 50% each. the optimal fermentation time of phospholipase D producing is 3d, its optimum transphosphatidylation-temperature is 32℃. its optimum transphosphatidylation pH value is 6.0,its optimum reaction buffer for pH=5.5 0.02M HAc -NaAC buffer, its Optimal metalion activator as Ca2 +, Zn2 +.