藏红花花瓣粗多糖的体外抗氧化活性研究

The antioxidant activity in vitro of crude polysaccharide from Crocus sativus petals

摘要:

采用水提醇沉法提取藏红花花瓣中的多糖，得率为3.75%。联合蒽酮-硫酸法和DNS法测得多糖纯度为68.34%。对粗多糖的体外抗氧化活性进行测定，结果表明，粗多糖具有一定的抗氧化活性，抗氧化能力指数（ORAC）值为0.572 μmol TE/mg；其对ABTS+、DPPH和羟自由基具有一定的清除能力，半数清除浓度EC50分别为76.26 μg/mL、165.5 μg/mL和880.4 μg/mL。

Abstract:

The yield of polysaccharide from Crocus sativus petals was 3.75% by water extraction and alcohol precipitation. The purity of crude polysaccharide was 68.34%, measured by anthrone-sulfuric acid method and DNS method. The antioxidant activity in vitro of crude polysaccharide was also determined. The results showed that crude polysaccharide exhibited certain antioxidant activity with the ORAC value of 0.572 μmol TE/mg, and had effect on scavenging ABTS+, DPPH and hydroxyl radical with the half elimination concentration (EC50) of 76.26 μg/mL, 165.5 μg/mL and 880.4 μg/mL respectively.