猪抑胃肽/葡萄糖依赖性促胰岛素释放肽(GIP)基因的克隆与组织表达分析

Cloning and Tissue Expression of gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) in Pigs

摘要:

抑胃肽/葡萄糖依赖性促胰岛素释放肽(GIP)是调节胰岛素释放的一种肠道分泌多肽。本研究采用RT-PCR方法，克隆了长白猪(Landrace) GIP基因全长cDNA序列, 并对其在家猪组织中的表达情况进行了分析。 结果显示: 长白猪GIP (pGIP) cDNA全长435 bp，编码144个氨基酸，即GIP前体蛋白；GIP前体蛋白含有信号肽序列，经蛋白酶水解后产生GIP成熟肽；与人、小鼠GIP表达图谱类似，GIP mRNA也在长白猪小肠及肾脏中有较高水平表达。猪GIP基因克隆与组织表达分析，将为探究GIP在家猪中的生理作用奠定基础。

Abstract:

The gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) shows the inhibitory effect on gastric acid secretion and incretin action. The aim of this study is to analyze the cDNA sequence of the pig GIP (pGIP) gene, and further examine the tissue expression of pGIP based on RT-PCR method. Result showed that pGIP cDNA is 532 bp in length and encodes a 144-amino acid GIP precursor which contains a signal peptide. RT-PCR assay showed that pGIP mRNA is highly expressed in kidney and gastrointestinal tract. Characterization of the pGIP gene will help to elucidate its physiological role.