水解胶原蛋白对枯草芽胞杆菌生长的影响

Effect of collagen hydrolysate on the growth of Bacillus subtilis

摘要:

研究水解胶原蛋白（Collagen hydrolysate, CH）对枯草芽胞杆菌（Bacillus subtilis）生长的影响，为CH在发酵领域的资源化应用提供理论基础。测定不同氮源（CH、蛋白胨、明胶、NaNO3、NH4Cl）对枯草芽胞杆菌生长的影响；考察CH和玉米浆的不同配比对枯草芽胞杆菌生长的影响；研究以CH为氮源时，不同碳源对枯草芽胞杆菌生长的影响；考查CH对其它细菌发酵的影响。研究发现，在CH为氮源条件下，枯草芽胞杆菌的发酵过程可顺利进行，且微生物的生物量可保持在较高水平；同玉米浆相比，CH能使枯草芽胞杆菌达到较高的生物量；以CH为氮源时，淀粉比葡萄糖、蔗糖更有利于枯草芽胞杆菌的生长；除了枯草芽胞杆菌以外，大肠杆菌（Escherichia coli ATCC 15489）和地衣芽胞杆菌（Bacillus licheniformis CICC 20031）也能有效利用CH。这表明，在实验条件下水解胶原蛋白（CH）可以作为多种微生物发酵的氮源。

Abstract:

In the present study, the effect of collagen hydrolysate (CH) on the growth of Bacillus subtilis was investigated for its valuable utilization in the fermentation industry. The influences of CH and other nitrogen sources, the proportion of CH and corn steep liquor, and the different carbon source at the present of CH on the growth of B. subtilis were significantly explored. Further, the effects of CH as nitrogen source on the fermentation for several bacteria was also investigated. With a positive effect on the biomass of Bacillus subtilis, CH was observed to be a suitable nitrogen source for the microbial fermentation. Comparing with corn steep liquor, CH exhibited a strong ability to improve the growth of Bacillus subtilis and keep its biomass in a high level. However, starch was a better carbon source than glucose and sucrose for the growth of Bacillus subtilis with the present of CH. Moreover, CH showed the significant value to be used as the nitrogen resource for the growth of Escherichia coli (ATCC 15489) and Bacillus licheniformis (CICC 20031). This suggests that CH possesses the potential to be utilized as a nitrogen resource for the growth of multiple microorganisms in the fermentation industry.