拟南芥RING finger结构域AtHHR1基因的功能初步研究

Primary Analysis of the Function of Arabidopsis thaliana RING finger AtHHR1

摘要:

拟南芥Highly Homologous RING domain 1基因（AtHHR1）编码一个含有RING finger结构域的E3连接酶。根据基因芯片表达谱推测该基因可能在参与热胁迫响应等方面起作用。为了研究该基因在响应热胁迫上的功能，鉴定了athhr1/ATHHR1互补转基因株系。对突变体、互补株系及野生型进行热胁迫处理，发现突变体的萌发率、叶绿素及脯氨酸含量高于野生型，而互补株系均低于野生型。经Real-time PCR发现，热诱导后拟南芥热信号通路中相关热激蛋白基因在突变体中表达比野生型中高。以上结果证明AtHHR1基因在拟南芥的热胁迫响应中起负调控作用。

Abstract:

Highly Homologous RING domain 1（AtHHR1）encodes a RING finger E3 ligase. It may play a role in participating heat stress response, according to publically available microarray data. To study the function of AtHHR1 under heat stress, 3 homozygous complementary transgenic lines athhr1/ATHHR1 were identified. The mutants, complementary transgenic lines and wild-type of Arabidopsis were used for heat stress experiment. Under the treatment of heat stress, the germination rate and relative chlorophyll and proline contents in mutant plants were higher than that in wild-type, while those in the complementary transgenic plants were all lower than that in wild-type. After heat stress, the expression levels of heat-related genes were higher in the mutant plants than in the wild-type. These results suggested that AtHHR1 gene played a negative regulatory role in Arabidopsis thaliana thermotolerance．