唐古特大黄内生放线菌分离鉴定及生防作用研究

Isolation of Endophytic Actinomycetes from Rheum tanguticum and Its Application in Biogical Control of Magnaporthe grisea

摘要:

以唐古特大黄为材料，采用匀浆涂布法进行内生放线菌的分离，并运用平板对峙法和生长速率法筛选内生放线菌的抑菌活性，最终获得一株具有抑制稻瘟病菌活性的内生放线菌4-21。通过形态学观察、生理生化特性检测和16S rDNA序列分析鉴定菌株4-21为Streptomyces albidoflavus 微白黄链霉菌；通过盆栽试验验证菌株4-21对稻瘟病菌的防治效果。结果表明，内生放线菌4-21在水稻稻瘟病的生物防治中具有潜在的应用价值。

Abstract:

Endophytic actinomycetes were isolated from Rheum tanguticum by spread plate method. Inhibitory activities of endophytic actinomycetes were determined by dual-culture inhibition on agar plate and growth rate method. As a result, Strain 4-21with high inhibitory effect on Magnaporthe grisea was found. The strain 4-21 was indentified as Streptomyces albidoflavus according to the morphology biochemical characterization and 16S rDNA gene sequence analysis. And its potted control effect was also investigated. Strain 4-21 deserved to be developed a kind of biocontrol agent.